On-chip high-speed sorting of micron-sized particles for high-throughput analysis

A new design of particle sorting chip is presented. The device employs a dielectrophoretic gate that deflects particles into one of two microfluidic channels at high speed. The device operates by focussing particles into the central streamline of the main flow channel using dielectrophoretic focussing. At the sorting junction (T- or Y-junction) two sets of electrodes produce a small dielectrophoretic force that pushes the particle into one or other of the outlet channels, where they are carried under the pressure-driven fluid flow to the outlet. For a 40mm wide and high channel, it is shown that 6micron diameter particles can be deflected at a rate of 300particles/s. The principle of a fully automated sorting device is demonstrated by separating fluorescent from non-fluorescent latex beads

Larval rearing, spat production and juvenile growth of the blood clam Anadara granosa

The blood clam A d r u gmtwsa spawned in the Shdl6lsh Hatchery Laboratory, Tuf icorin on two occasions. The fertilised eggs measured 50-60~in diameter, morula larvae developed in 3 4h m and the trochophorc stage was reached in 5 hrs. The straigb t hinge stage was attained in 20-26 hrs after fertilimtion and these larvae measured 8 3I~en ah and 65.5~h eight. Advanced umbo stage was reached on day 12 (size 155.3 x 140.5~) and on day 16, majority of the larvae were in pedivcliger stage with an average size of 182.7 x 162.9~. SettIement began on day 16 and majority of the larvae were set on day 18. The growth of the spat in the hatchery is described by the exponential equation L - 0.0002739 D - where L is length in mm and D. days. On day 59, tbe spat attained an average size of 2.42 x 1.70 mm. A total of 8090 spat were produced. During tha nursery rearing in the field, the seed clam attained 20 mm average length in the following 5 months. In India, A.grund~llsecd were grodwdfor the Arst time. The significance of this study forthe mass production of the blood clam seed in the bat~herya nd its relevma to undertake blood clam culture are highlighted

Experience paper on the operation of pilot project on oyster culture at Tuticorin

The technology of oyster culture was developed in India at the Central Marine Fisheries Research Institute. An experimental farm was established at Tuticorin bay in an area of 0.25 hectares. Different growout methods have been tried and it has been assessed that the shell string method could be practised with relatively low cost inputs with a production rate comparable to highly efficient systems such as rack and tray method or raft culture

Onset of magnetism in B2 transition metals aluminides

Ab initio calculation results for the electronic structure of disordered bcc Fe(x)Al(1-x) (0.4<x<0.75), Co(x)Al(1-x) and Ni(x)Al(1-x) (x=0.4; 0.5; 0.6) alloys near the 1:1 stoichiometry, as well as of the ordered B2 (FeAl, CoAl, NiAl) phases with point defects are presented. The calculations were performed using the coherent potential approximation within the Korringa-Kohn-Rostoker method (KKR-CPA) for the disordered case and the tight-binding linear muffin-tin orbital (TB-LMTO) method for the intermetallic compounds. We studied in particular the onset of magnetism in Fe-Al and Co-Al systems as a function of the defect structure. We found the appearance of large local magnetic moments associated with the transition metal (TM) antisite defect in FeAl and CoAl compounds, in agreement with the experimental findings. Moreover, we found that any vacancies on both sublattices enhance the magnetic moments via reducing the charge transfer to a TM atom. Disordered Fe-Al alloys are ferromagnetically ordered for the whole range of composition studied, whereas Co-Al becomes magnetic only for Co concentration >0.5.Comment: 11 pages with 9 embedded postscript figures, to be published in Phys.Rev.

Exploited marine fishery resources off Tuticorin along the Gulf of Mannar coast

Tuticorin coast of Gulf of Mannar is endowed with rocky bottom, coral reefs and sea grass beds with characteristic flora and fauna. It also acts as home for several endangered marine mammals, sea cows and marine turtles. These resources were exploited by a variety of gears during 2000-2005

Outer-Sphere Contributions to the Electronic Structure of Type Zero Copper Proteins

Bioinorganic canon states that active-site thiolate coordination promotes rapid electron transfer (ET) to and from type 1 copper proteins. In recent work, we have found that copper ET sites in proteins also can be constructed without thiolate ligation (called “type zero” sites). Here we report multifrequency electron paramagnetic resonance (EPR), magnetic circular dichroism (MCD), and nuclear magnetic resonance (NMR) spectroscopic data together with density functional theory (DFT) and spectroscopy-oriented configuration interaction (SORCI) calculations for type zero Pseudomonas aeruginosa azurin variants. Wild-type (type 1) and type zero copper centers experience virtually identical ligand fields. Moreover, O-donor covalency is enhanced in type zero centers relative that in the C112D (type 2) protein. At the same time, N-donor covalency is reduced in a similar fashion to type 1 centers. QM/MM and SORCI calculations show that the electronic structures of type zero and type 2 are intimately linked to the orientation and coordination mode of the carboxylate ligand, which in turn is influenced by outer-sphere hydrogen bonding

Distribution and exploitation of oyster resources along the southeast and southwest coasts of India

The oysters are sedentary bivalve molluscs which are gregarious and found in aggregates attached by their right shell valve to hard substrata, forming oyster beds or oyster banks in varied environments, intertidal and subtidal zones in shallow coastal waters, bays, creeks, lagoons, backwaters and estuarine environment. In India, natural stocks of oysters are exploited on a small scale at a number of places as a subsistence fishery and oysters are not cultured commercially

Survey of Valinokkam Bay and adjoining area to assess its suitability for integrated sea farming — A report

The Valinokkam Bay and the adjoining area, east of the Bay surveyed, lie between Lat. 9°9' N and 9° 12' N and Long. 78°30'E and 78°42'E . The available information indicates that the bay and the adjoining grounds in the sea are highly productive and suitable for sea farming activities

Active Brownian Particles. From Individual to Collective Stochastic Dynamics

We review theoretical models of individual motility as well as collective dynamics and pattern formation of active particles. We focus on simple models of active dynamics with a particular emphasis on nonlinear and stochastic dynamics of such self-propelled entities in the framework of statistical mechanics. Examples of such active units in complex physico-chemical and biological systems are chemically powered nano-rods, localized patterns in reaction-diffusion system, motile cells or macroscopic animals. Based on the description of individual motion of point-like active particles by stochastic differential equations, we discuss different velocity-dependent friction functions, the impact of various types of fluctuations and calculate characteristic observables such as stationary velocity distributions or diffusion coefficients. Finally, we consider not only the free and confined individual active dynamics but also different types of interaction between active particles. The resulting collective dynamical behavior of large assemblies and aggregates of active units is discussed and an overview over some recent results on spatiotemporal pattern formation in such systems is given.Comment: 161 pages, Review, Eur Phys J Special-Topics, accepte

Gender-dependent differences in plasma matrix metalloproteinase-8 elevated in pulmonary tuberculosis.

Tuberculosis (TB) remains a global health pandemic and greater understanding of underlying pathogenesis is required to develop novel therapeutic and diagnostic approaches. Matrix metalloproteinases (MMPs) are emerging as key effectors of tissue destruction in TB but have not been comprehensively studied in plasma, nor have gender differences been investigated. We measured the plasma concentrations of MMPs in a carefully characterised, prospectively recruited clinical cohort of 380 individuals. The collagenases, MMP-1 and MMP-8, were elevated in plasma of patients with pulmonary TB relative to healthy controls, and MMP-7 (matrilysin) and MMP-9 (gelatinase B) were also increased. MMP-8 was TB-specific (p<0.001), not being elevated in symptomatic controls (symptoms suspicious of TB but active disease excluded). Plasma MMP-8 concentrations inversely correlated with body mass index. Plasma MMP-8 concentration was 1.51-fold higher in males than females with TB (p<0.05) and this difference was not due to greater disease severity in men. Gender-specific analysis of MMPs demonstrated consistent increase in MMP-1 and -8 in TB, but MMP-8 was a better discriminator for TB in men. Plasma collagenases are elevated in pulmonary TB and differ between men and women. Gender must be considered in investigation of TB immunopathology and development of novel diagnostic markers