Integrated high-content quantification of intracellular ROS levels and mitochondrial morphofunction

Oxidative stress arises from an imbalance between the production of reactive oxygen species (ROS) and their removal by cellular antioxidant systems. Especially under pathological conditions, mitochondria constitute a relevant source of cellular ROS. These organelles harbor the electron transport chain, bringing electrons in close vicinity to molecular oxygen. Although a full understanding is still lacking, intracellular ROS generation and mitochondrial function are also linked to changes in mitochondrial morphology. To study the intricate relationships between the different factors that govern cellular redox balance in living cells, we have developed a high-contentmicroscopy-based strategy for simultaneous quantification of intracellular ROS levels and mitochondrial morphofunction. Here, we summarize the principles of intracellular ROS generation and removal, and we explain the major considerations for performing quantitative microscopy analyses of ROS and mitochondrial morphofunction in living cells. Next, we describe our workflow, and finally, we illustrate that a multiparametric readout enables the unambiguous classification of chemically perturbed cells as well as laminopathy patient cells

Soil survey in the Larisa area, Greece

This article is based on a soil survey carried out in the Larisa area, Greece, by staff and students of ITC's soil survey group, in cooperation with the Greek Institute of Soil Classification and Mapping. A 1:100 000 scale soil map is included. Drainage patterns in one of the major landtypes in the area, extending over heterogeneous Tertiary deposits, were also investigated. A procedure for combining the colour-coded soil map with Landsat MSS data is presented. -from Authors</p