A New Methodology for Recognition of Milling Features from STEP File

In recent years, various researchers have come up with different ways and means to integrate CAD and CAM. Automatic feature recognition (AFR) from a CAD solid model for down stream applications like process planning and NC program, greatly contribute to the level of integration. When generating G&M codes from CAD DXF file, it leads to the loss of geometric information and the user is to edit and fills the details of the lost data. STEP is an international standard for geometric and non geometric data transfer between CAD, CAE and CAM and it replaces the IGES and DXF. For that reason this paper proposes an automatic feature recognition methodology to develop a feature recognition system using STEP file. The proposed methodology is developed for 3D prismatic parts that are modeled any CAD software having STEP output file format. A JAVA program is used to implement the geometric data extraction algorithm, which has been developed for extracting the geometric information from the STEP file. A feature recognition algorithm is used to recognize the different features of the part such as slot, pocket etc based on geometric reasoning approach by taking B-rep data base as input. The authors present an example to demonstrate the application of the proposed methodology

Response of cardamom (Elettaria cardamomum Maton) seedlings to vesicular arbuscular mycorrhizal fungi

The growth response of cardamom (Elettaria cardamomum) seedlings (cv, Malabar) to 13 different vesicular arbuscular mycorrhizal fungi was tested under mat house conditions at Bangalore, India, In general, seedlings inoulated with the fungi grew taller, had more number of leaves and tillers, increased seedling biomass and uptake of nutrients compared to control seedlings, Among the various mycorrhizal fungi tested, seedlings inoculated with Gigaspora margarita and Glomus monosporum exhibited significantly higher growth with increased uptake of nutrients, &nbsp

Response of cardamom (Elettaria cardamomum Maton) seedlings to vesicular arbuscular mycorrhizal fungi

The growth response of cardamom (Elettaria cardamomum) seedlings (cv, Malabar) to 13 different vesicular arbuscular mycorrhizal fungi was tested under mat house conditions at Bangalore, India, In general, seedlings inoulated with the fungi grew taller, had more number of leaves and tillers, increased seedling biomass and uptake of nutrients compared to control seedlings, Among the various mycorrhizal fungi tested, seedlings inoculated with Gigaspora margarita and Glomus monosporum exhibited significantly higher growth with increased uptake of nutrients, &nbsp

Response of cardamom (Elettaria cardamomum Maton) seedlings to vesicular arbuscular mycorrhizal fungi

The growth response of cardamom (Elettaria cardamomum) seedlings (cv, Malabar) to 13 different vesicular arbuscular mycorrhizal fungi was tested under mat house conditions at Bangalore, India, In general, seedlings inoulated with the fungi grew taller, had more number of leaves and tillers, increased seedling biomass and uptake of nutrients compared to control seedlings, Among the various mycorrhizal fungi tested, seedlings inoculated with Gigaspora margarita and Glomus monosporum exhibited significantly higher growth with increased uptake of nutrients, &nbsp

Systems analysis of bioenergetics and growth of the extreme halophile Halobacterium salinarum

Halobacterium salinarum is a bioenergetically flexible, halophilic microorganism that can generate energy by respiration, photosynthesis, and the fermentation of arginine. In a previous study, using a genome-scale metabolic model, we have shown that the archaeon unexpectedly degrades essential amino acids under aerobic conditions, a behavior that can lead to the termination of growth earlier than necessary. Here, we further integratively investigate energy generation, nutrient utilization, and biomass production using an extended methodology that accounts for dynamically changing transport patterns, including those that arise from interactions among the supplied metabolites. Moreover, we widen the scope of our analysis to include phototrophic conditions to explore the interplay between different bioenergetic modes. Surprisingly, we found that cells also degrade essential amino acids even during phototropy, when energy should already be abundant. We also found that under both conditions considerable amounts of nutrients that were taken up were neither incorporated into the biomass nor used as respiratory substrates, implying the considerable production and accumulation of several metabolites in the medium. Some of these are likely the products of forms of overflow metabolism. In addition, our results also show that arginine fermentation, contrary to what is typically assumed, occurs simultaneously with respiration and photosynthesis and can contribute energy in levels that are comparable to the primary bioenergetic modes, if not more. These findings portray a picture that the organism takes an approach toward growth that favors the here and now, even at the cost of longer-term concerns. We believe that the seemingly "greedy" behavior exhibited actually consists of adaptations by the organism to its natural environments, where nutrients are not only irregularly available but may altogether be absent for extended periods that may span several years. Such a setting probably predisposed the cells to grow as much as possible when the conditions become favorable

Synthesis and Characterization of PLA/Luffa Cylindrica Composite Films

In recent days, natural fibers have been replaced with synthetic fibers as an alternative material for the reinforcement in the polymer composites due to their renewability and sustainability. The aim of the present work is to study the performance of using natural fiber powder in the preparation of composite films. The fiber powder used in the present study is extracted from the Luffa cylindrica plant. Fourier Transform Infrared (FTIR) spectrum is utilized for the identification of functional groups in the fabricated composite film. Scanning Electron Microscopy (SEM) analysis is carried out to observe the bonding between matrix and reinforcement in the composite films. Thermal degradation behaviour of the composite film is also studied by the Thermo-gravimetric Analysis (TGA). From the studies, it was observed that the natural fiber reinforced composite film were able to withstand higher temperatures. The fabricated composites films can be effectively used for different applications where there is a necessity of bio polymers

Response of cardamom (Elettaria cardamomum Maton) seedlings to vesicular arbuscular mycorrhizal fungi

The growth response of cardamom (Elettaria cardamomum) seedlings (cv, Malabar) to 13 different vesicular arbuscular mycorrhizal fungi was tested under mat house conditions at Bangalore, India, In general, seedlings inoulated with the fungi grew taller, had more number of leaves and tillers, increased seedling biomass and uptake of nutrients compared to control seedlings, Among the various mycorrhizal fungi tested, seedlings inoculated with Gigaspora margarita and Glomus monosporum exhibited significantly higher growth with increased uptake of nutrients, &nbsp

Response of cardamom (Elettaria cardamomum Maton) seedlings to vesicular arbuscular mycorrhizal fungi

The growth response of cardamom (Elettaria cardamomum) seedlings (cv, Malabar) to 13 different vesicular arbuscular mycorrhizal fungi was tested under mat house conditions at Bangalore, India, In general, seedlings inoulated with the fungi grew taller, had more number of leaves and tillers, increased seedling biomass and uptake of nutrients compared to control seedlings, Among the various mycorrhizal fungi tested, seedlings inoculated with Gigaspora margarita and Glomus monosporum exhibited significantly higher growth with increased uptake of nutrients, &nbsp

High quality factor 1-D Er 3+ -activated dielectric microcavity fabricated by RF-sputtering

Rare earth-activated 1-D photonic crystals were fabricated by RF-sputtering technique. The cavity is constituted by an Er3+-doped SiO2 active layer inserted between two Bragg reflectors consisting of ten pairs of SiO2/TiO2 layers. Scanning electron microscopy is employed to put in evidence the quality of the sample, the homogeneities of the layers thickness and the good adhesion among them. Near infrared transmittance and variable angle reflectance spectra confirm the presence of a stop band from 1500 nm to 2000 nm with a cavity resonance centered at 1749 nm at 0° and a quality factor of 890. The influence of the cavity on the 4I13/2 -> 4I15/2 emission band of Er3+ ion is also demonstrated

Tryptophan fluorescence quenching as a binding assay to monitor protein conformation changes in the membrane of intact mitochondria

Intrinsic protein fluorescence is due to aromatic amino acids, mainly tryptophan, which can be selectively measured by exciting at 295 nm. Changes in emission spectra of tryptophan are due to the protein conformational transitions, subunit association, ligand binding or denaturation, which affect the local environment surrounding the indole ring. In this study, tryptophan fluorescence was monitored in intact mitochondria at 333 nm following excitation at 295 nm in presence of insecticides using spectrofluorometer. Methyl-parathion, carbofuran, and endosulfan induced Trp fluorescence quenching and release of cytochrome c when incubated with the mitochondria, except fenvalarate. Mechanism of insecticide-induced mitochondrial toxicity for the tested insecticides has been discussed. Reduction in the intensity of tryptophan emission spectra of mitochondrial membrane proteins in presence of an increasing concentration of a ligand can be used to study the interaction of insecticides/drugs with the intact mitochondria. Furthermore, this assay can be readily adapted for studying protein–ligand interactions in intact mitochondria and in other cell organelles extending its implications for pesticide and pharma industry and in drug discovery