Flexible Support Stanchion

Figure 1 shows the assembly drawing of the Central Calorimeter Cryostat Flexible Support Stanchion. Figures 2 and 3 show the Flexible Support STanchion in detail. These Stanchions support the cryostat safely, reduce the heat load to the cryostat from the ambient by a factor of more than ten, provide a spring like action that reduce the loads created by thermal contraction of the cryostat and position the cryostate accurately. Table 1 shows all of the details of the Flexible Support system for the C.C. Cryostat

Central Calorimeter Support Cradle Jack Failure Analysis

The Central Calorimeter and its support cradle are to be supported by either hydraulic or mechanical jacks. If hydraulics are used, each support will use two hydraulically coupled jacks with two out of the four supports hydraulically coupled giving the effect of a three point support system. If mechanical jacks are used, all four points are used for support. Figure 2 shows two examples of jack placement on a 3.5 inch support plate. These two support scenarios lead to five jack failure cases to be studied. This report deals with the way in which a 0.25 inch drop (failed jack) at one support affects the stresses in the cradle. The stresses from each failure case were analyzed in two ways. First, stress factors, defined as quotients of stress intensities of the failed case with respect to the static case, were generated and then, hand calculations similar to those in Engineering Note 3740.215-EN-14 were done using the reaction forces from the failed case

PVP2008-61205 NATURAL FLAW SHAPE DEVELOPMENT DUE TO STRESS CORROSION CRACKING

ABSTRACT Typical ASME Section XI subcritical cracking analyses assume an idealized flaw shape driven by stress intensity factors developed for semi-elliptical shaped flaws. Recent advanced finite element analyses (AFEA) conducted by both the US NRC and the nuclear industry for long circumferential indications found in the pressurizer nozzle dissimilar metal welds at the Wolf Creek power plant, suggest that the semielliptical flaw assumption may be overly conservative in some cases. The AFEA methodology that was developed allowed the progression of a planar flaw subjected to typical SCC-type growth laws by calculating stress intensity factors at every nodal point along the crack front, and incrementally advancing the crack front in a more natural manner. Typically crack growth analyses increment the semi-elliptical flaw by considering only the stress intensity factor at the deepest and surface locations along the crack front, while keeping the flaw shape semi-elliptical. In this paper, a brief background to the AFEA methodology and the analyses conducted in the Wolf Creek effort will be discussed. In addition, the natural behavior of surface cracks under normal operating conditions (plus welding residual stress) will be investigated and compared to the semi-elliptical assumption. Conclusions on the observation of when semi-elliptical flaw assumptions are appropriate will be made. These observations will add insight into the conservatism of using an idealized flaw shape assumption. INTRODUCTION In October 2006, circumferential indications were located by ultrasonic testing (UT) in three of the pressurizer nozzle dissimilar metal (DM) welds at the Wolf Creek nuclear power plant. The indications located were relatively long circumferential defects in Alloy 82/182 dissimilar metal welds. In one case the flaw was sized at 43% of the circumference and 26% deep. Using ASME Section XI type analyses, Emc 2 and NRC staff estimated the times to both leakage and rupture fo

The effect of 14 weeks of vitamin D3 supplementation on antimicrobial peptides and proteins in athletes

Heavy training is associated with increased respiratory infection risk and antimicrobial proteins are important in defence against oral and respiratory tract infections. We examined the effect of 14 weeks of vitamin D3 supplementation (5000 IU/day) on the resting plasma cathelicidin concentration and the salivary secretion rates of secretory immunoglobulin A (SIgA), cathelicidin, lactoferrin and lysozyme in athletes during a winter training period. Blood and saliva were obtained at the start of the study from 39 healthy men who were randomly allocated to vitamin D3 supplement or placebo. Blood samples were also collected at the end of the study; saliva samples were collected after 7 and 14 weeks. Plasma total 25(OH)D concentration increased by 130% in the vitamin D3 group and decreased by 43% in the placebo group (both P=0.001). The percentage change of plasma cathelicidin concentration in the vitamin D3 group was higher than in the placebo group (P=0.025). Only in the vitamin D3 group, the saliva SIgA and cathelicidin secretion rates increased over time (both P=0.03). A daily 5000 IU vitamin D3 supplement has a beneficial effect in up-regulating the expression of SIgA and cathelicidin in athletes during a winter training period which could improve resistance to respiratory infections

S100A4 Elevation Empowers Expression of Metastasis Effector Molecules in Human Breast Cancer.

Many human glandular cancers metastasize along nerve tracts, but the mechanisms involved are generally poorly understood. The calcium-binding protein S100A4 is expressed at elevated levels in human cancers, where it has been linked to increased invasion and metastasis. Here we report genetic studies in a Drosophila model to define S100A4 effector functions that mediate metastatic dissemination of mutant Ras-induced tumors in the developing nervous system. In flies overexpressing mutant RasVal12 and S100A4, there was a significant increase in activation of the stress kinase JNK and production of the matrix metalloproteinase MMP1. Genetic or chemical blockades of JNK and MMP1 suppressed metastatic dissemination associated with S100A4 elevation, defining required signaling pathway(s) for S100A4 in this setting. In clinical specimens of human breast cancer, elevated levels of the mammalian paralogs MMP2, MMP9, and MMP13 are associated with a 4- to 9-fold relative decrease in patient survival. In individual tumors, levels of MMP2 and MMP13 correlated more closely with levels of S100A4, whereas MMP9 levels correlated more closely with the S100 family member S100P. Overall, our results suggest the existence of evolutionarily conserved pathways used by S100A4 to promote metastatic dissemination, with potential prognostic and therapeutic implications for metastasis by cancers that preferentially exploit nerve tract migration routes. Cancer Res; 77(3); 1-10. ©2016 AACR

Determination of the Elastic-Plastic Fracture Mechanics Z-factor for Alloy 182 Weld Metal Flaws for Use

ABSTRACT One of the ways that the ASME Section XI code incorporates elastic-plastic fracture mechanics (EPFM) in the Section XI Appendix C flaw evaluation procedures for circumferential cracks is through a parameter called Z-factor. This parameter allows the simpler limit-load (or net-section-collapse) solutions to be used with a multiplier from EPFM analyses. Traditionally the EPFM solution was determined by using the GE-EPRI Jestimation scheme to determine the maximum load by EPFM, and Z = limit load / EPFM solution. The Z-factor is a function of the material toughness as well as the pipe diameter. With the advent of primary water stress-corrosion cracks (PWSCC) in pressurized water reactor (PWR) dissimilar metal welds (DMW), there is a need to develop Z-factors for Alloy 82/182 nickel-based alloy welds that are susceptible to such cracks. Although there have been Z-factor solutions for cracks in stainless and ferritic pipe butt welds, the DMW are somewhat different in that there is a much lower yield strength material on one side of the weld (typically forged or wrought 304 stainless steel) and on the other side of the weld the low alloy steel has a much higher strength than even the weld metal. This paper shows how 3D finite element analyses were used for a particular pipe size to determine the sensitivity of the crack location in the Alloy 182 weldment (crack in the center of weld, or closer to the stainless or low alloy steel sides), and how an appropriate stress-strain curve was determined for use in the J-estimation schemes. A Z-factor as a function of the pipe diameter was then calculated using the LBB.ENG2 J estimation scheme using the appropriate stress-strain curves from the finite element analysis. The LBB.ENG2 analysis was used rather than the GE-EPRI estimation scheme since it has been found that the LBB.ENG2 analysis is more accurate when compared with full-scale pipe tests. From past work, the GE-EPRI method was found to be the most conservative of the Jestimation schemes in predicting the maximum loads for circumferential flaws when compared to full-scale circumferentially cracked-pipe tests. The proposed Z-factor relationship should be restricted to normal operating temperatures (above 200C) with low H 2 concentrations, where the Alloy 182 weld metal exhibits high toughness. BACKGROUND The occurrence of PWSCC in dissimilar metal pipe butt welds using Alloy 82 and 182 weld metals has been increasing and evaluation procedures are needed in Section XI of the ASME Boiler and Pressure Vessel Code. Article IWB-3640 and Appendix C (2004 Edition) cover elastic-plastic fracture mechanics (EPFM) analyses for ferritic and austenitic stainless steels and their welds. Currently no criterion exists for cracks in Alloy 82/182 weld metals

Metastasis-inducing proteins are widely expressed in human brain metastases and associated with intracranial progression and radiation response

Background:Understanding the factors that drive recurrence and radiosensitivity in brain metastases would improve prediction of outcomes, treatment planning and development of therapeutics. We investigated the expression of known metastasis-inducing proteins in human brain metastases.Methods:Immunohistochemistry on metastases removed at neurosurgery from 138 patients to determine the degree and pattern of expression of the proteins S100A4, S100P, AGR2, osteopontin (OPN) and the DNA repair marker FANCD2. Validation of significant findings in a separate prospective series with the investigation of intra-tumoral heterogeneity using image-guided sampling. Assessment of S100A4 expression in brain metastatic and non-metastatic primary breast carcinomas.Results:There was widespread staining for OPN, S100A4, S100P and AGR2 in human brain metastases. Positive staining for S100A4 was independently associated with a shorter time to intracranial progression after resection in multivariate analysis (hazard ratio for negative over positive staining=0.17, 95% CI: 0.04-0.74, P=0.018). S100A4 was expressed at the leading edge of brain metastases in image guided sampling and overexpressed in brain metastatic vs non-brain metastatic primary breast carcinomas. Staining for OPN was associated with a significant increase in survival time after post-operative whole-brain radiotherapy in retrospective (OPN negative 3.43 months, 95% CI: 1.36-5.51 vs OPN positive, 11.20 months 95% CI: 7.68-14.72, Log rank test, P<0.001) and validation populations.Conclusions:Proteins known to be involved in cellular adhesion and migration in vitro, and metastasis in vivo are significantly expressed in human brain metastases and may be useful biomarkers of intracranial progression and radiosensitivity

IPIRG-2 Task 1 - Pipe System Experiments with Circumferential Cracks in Straight-Pipe Locations. Final report:September 1991--November 1995

This report presents the results from Task 1 of the Second International Piping Integrity Research Group (IPIRG-2) program. The IPIRG-2 program is an international group program managed by the US Nuclear Regulatory Commission (US NRC) and funded by a consortium of organizations from 15 nations including: Bulgaria, Canada, Czech Republic, France, Hungary, Italy, Japan, Republic of Korea, Lithuania, Republic of China, Slovak Republic, Sweden, Switzerland, the United Kingdom, and the United States. The objective of the program was to build on the results of the IPIRG-1 and other related programs by extending the state-of-the-art in pipe fracture technology through the development of data needed to verify engineering methods for assessing the integrity of nuclear power plant piping systems that contain defects. The IPIRG-2 program included five main tasks: Task 1 - Pipe System Experiments with Flaws in Straight Pipe and Welds Task 2 - Fracture of Flawed Fittings Task 3 - Cyclic and Dynamic Load Effects on Fracture Toughness Task 4 - Resolution of Issues From IPIRG-1 and Related Programs Task 5 - Information Exchange Seminars and Workshops, and Program Management. The scope of this report is to present the results from the experiments and analyses associated with Task 1 (Pipe System Experiments with Flaws in Straight Pipe and Welds). The rationale and objectives of this task are discussed after a brief review of experimental data which existed after the IPIRG-1 program