Decomposition-based recovery of absorbers in turbid media

We suggest that the concept of the point-spread function traditionally used to predict the blurred image pattern of various light sources embedded inside turbid media can be generalized under certain conditions to predict also the presence and location of spatially localized absorbing inhomogeneities based on shadow point-spread functions associated with each localized absorber in the medium. The combined image obtained from several absorbers can then be decomposed approximately into the arithmetic sums of these individual shadow point-spread functions with suitable weights that can be obtained from multiple-regression analysis. This technique permits the reconstruction of the location of absorbers

The Function of a Spindle Checkpoint Gene bub-1 in C. elegans Development

BACKGROUND:The serine/threonine kinase BUB1 (Budding Uninhibited by Benzimidazole 1) was originally identified in yeast as a checkpoint protein, based on its mutant's incapacity of delaying the cell cycle in response to loss of microtubules. Our understanding of its function is primarily from studies carried out in yeast S. cerevisiae. It has been shown that it is a component of the mitotic spindle checkpoint and regulates the separation of sister chromatids through its downstream molecules. However, its roles in multi-cellular organisms remain unclear. METHODS AND FINDINGS:In nematode C. elegans, rapid cell divisions primarily occur in embryos and in germline of postembryonic larvae and adults. In addition, a select set of cells undergo a few rounds of cell division postembryonically. One common phenotype associated with impaired cell division is described as Stu (Sterile and Uncoordinated) [1], [2]. We conducted a genetic screen for zygotic mutants that displayed Stu phenotype in C. elegans. We isolated seven Stu mutants that fell into five complementation groups. We report here that two mutations, FanWang5 (fw5) and FanWang8 (fw8) affect the bub-1 gene, a homolog of yeast BUB1. Both mutant alleles of fw5 and fw8 exhibited variable behavioral defects, including developmental arrest, uncoordination and sterility. The number of postembryonically born neurons in the ventral cord decreased and their axon morphology was abnormal. Also, the decrease of neurons in the ventral cord phenotype could not be suppressed by a caspase-3 loss-of-function mutant. In addition, bub-1(fw5 and fw8) mutants showed widespread effects on postembryonic development in many cell lineages. We found that bub-1 functioned maternally in several developmental lineages at the embryonic stage in C. elegans. Studies in yeast have shown that BUB1 functions as a spindle checkpoint protein by regulating the anaphase promoting complex/cyclosome (APC/C). We performed double mutant analysis and observed that bub-1 genetically interacted with several downstream genes, including fzy-1/CDC20, mat-2/APC1 and emb-27/APC6. CONCLUSIONS:Our results demonstrate a conserved role of bub-1 in cell-cycle regulation and reveal that C. elegans bub-1 is required both maternally and zygotically. Further, our genetic analysis is consistent with that the function of bub-1 in C. elegans is likely similar to its yeast and mammalian homologs

Dynamic modeling and structural optimization of a bistable electromagnetic vibration energy harvester

A novel bistable electromagnetic vibration energy harvester (BEMH) is constructed and optimized in this study, based on a nonlinear system consisting mainly of a flexible membrane and a magnetic spring. A large-amplitude transverse vibration equation of the system is established with the general nonlinear geometry and magnetic force. Firstly, the mathematical model, considering the higher-order nonlinearities given by nonlinear Galerkin method, is applied to a membrane with a co-axial magnet mass and magnetic spring. Secondly, the steady vibration response of the membrane subjected to a harmonic base motion is obtained, and then the output power considering electromagnetic effect is analytically derived. On this basis, a parametric study in a broad frequency domain has been achieved for the BEMH with different radius ratios and membrane thicknesses. It is demonstrated that model predictions are both in close agreement with results from the finite element simulation and experiment data. Finally, the proposed efficient solution method is used to obtain an optimizing strategy for the design of multi-stable energy harvesters with the similar flexible structure

A Key Commitment Step in Erythropoiesis Is Synchronized with the Cell Cycle Clock through Mutual Inhibition between PU.1 and S-Phase Progression

During red blood cell development, differentiation and cell cycle progression are intimately and uniquely linked through interdependent mechanisms involving the erythroid transcriptional suppressor PU.1 and the cyclin-dependent kinase inhibitor p57KIP2

Procalcitonin and proinflammatory cytokine interactions in sepsis

Immunoneutralization of procalcitonin (ProCT), a putative mediator of sepsis, has been shown to increase survival in an animal model of sepsis. To better understand the role that ProCT plays in the sepsis cascade, we studied the relationship of this hormone to the proximal proinflammatory mediators, IL-1β and TNFα. Hamsters were made septic by i.p. implantation of Escherichia coli-impregnated agar pellets. A time line study of serum IL-β, TNFα, and ProCT levels showed that the increase in the cytokines was transient and less than 2-fold over baseline, whereas ProCT increased \u3e100-fold by 12 h and remains elevated through 24 h. TNFα (400 μg/kg) was injected into healthy animals, inducing an elevation in ProCT that was 25-fold greater than controls. ProCT (30 μg/kg) was given to healthy and septic animals. In healthy animals, there was no significant elevation in serum IL-1β or TNFα levels. In septic animals, IL-1β was modestly blunted at 3 h but not at 12 h, and there was no change in TNFα levels. ProCT did not initiate or enhance IL-1β or TNFα expression; however, the massive and sustained elevation of this hormone seen in sepsis can be induced by the proximal cytokine, TNFα. This study suggests that ProCT is a secondary mediator that might augment and amplify but does not initiate the septic response. Immunoneutralization of ProCT may prove to be an important clinical strategy, in view of its sustained elevation and the difficulty in initiating therapy for sepsis during the early phases of illness

Roles of the kinase TAK1 in CD40-mediated effects on vascular oxidative stress and neointima formation after vascular injury.

Although TAK1 has been implicated in inflammation and oxidative stress, its roles in vascular smooth muscle cells (VSMCs) and in response to vascular injury have not been investigated. The present study aimed to investigate the role of TAK1 in modulating oxidative stress in VSMCs and its involvement in neointima formation after vascular injury. Double immunostaining reveals that vascular injury induces a robust phosphorylation of TAK1 (Thr187) in the medial VSMCs of injured arteries in wildtype mice, but this effect is blocked in CD40-deficient mice. Upregulation of TAK1 in VSMCs is functionally important, as it is critically involved in pro-oxidative and pro-inflammatory effects on VSMCs and eventual neointima formation. In vivo, pharmacological inhibition of TAK1 with 5Z-7-oxozeaenol blocked the injury-induced phosphorylation of both TAK1 (Thr187) and NF-kB/p65 (Ser536), associated with marked inhibition of superoxide production, 3-nitrotyrosine, and MCP-1 in the injured arteries. Cell culture experiments demonstrated that either siRNA knockdown or 5Z-7-oxozeaenol inhibition of TAK1 significantly attenuated NADPH oxidase activation and superoxide production induced by CD40L/CD40 stimulation. Co-immunoprecipitation experiments indicate that blockade of TAK1 disrupted the CD40L-induced complex formation of p22phox with p47phox, p67phox, or Nox4. Blockade of TAK1 also inhibited CD40L-induced NF-kB activation by modulating IKKα/β and NF-kB p65 phosphorylation and this was related to reduced expression of proinflammatory genes (IL-6, MCP-1 and ICAM-1) in VSMCs. Lastly, treatment with 5Z-7-oxozeaenol attenuated neointimal formation in wire-injured femoral arteries. Our findings demonstrate previously uncharacterized roles of TAK1 in vascular oxidative stress and the contribution to neointima formation after vascular injury

A high performance contra-rotating energy harvester and its wireless sensing application toward green and maintain free vehicle monitoring

Intelligent transportation necessitates advanced perception and cognitive systems that can provide continuous feedback from the vehicle. However, sensors relying on batteries face challenges such as high maintenance costs and environmental issues due to the limited lifespan of the power source. To overcome these challenges, this paper reports an efficient battery-free solution for transportation monitoring. The solution utilizes a speed-amplified rotary energy harvester (SAREH) to power various wireless Bluetooth sensors, enabling continuous monitoring of the vehicle's motion state. The SAREH combines a contra-rotating mechanism with a friction pendulum, resulting in excellent power output in a compact design. Experimental results demonstrate the ability of SAREH to extract power from vehicles operating at speeds ranging from 180 to 1260 rpm. The maximum power output and corresponding power density are measured as 712 mW and 34 mW cm−3, respectively. The prototype successfully powers portable electronics and supports battery-free navigation, triaxial acceleration, and temperature multi-sensors during real road and railway simulation tests. Additionally, the SAREH operates as a highly sensitive speed sensor and an early-warning system for detecting the vehicle's motion state. These results represent a significant advancement in intelligent transportation systems by showcasing the practicality of self-powered wireless monitoring capabilities on vehicles