Purification and electron cryomicroscopy of coronavirus particles.

Intact, enveloped coronavirus particles vary widely in size and contour, and are thus refractory to study by traditional structural means such as X-ray crystallography. Electron microscopy (EM) overcomes some problems associated with particle variability and has been an important tool for investigating coronavirus ultrastructure. However, EM sample preparation requires that the specimen be dried onto a carbon support film before imaging, collapsing internal particle structure in the case of coronaviruses. Moreover, conventional EM achieves image contrast by immersing the specimen briefly in heavy-metal-containing stain, which reveals some features while obscuring others. Electron cryomicroscopy (cryo-EM) instead employs a porous support film, to which the specimen is adsorbed and flash-frozen. Specimens preserved in vitreous ice over holes in the support film can then be imaged without additional staining. Cryo-EM, coupled with single-particle image analysis techniques, makes it possible to examine the size, structure and arrangement of coronavirus structural components in fully hydrated, native virions. Two virus purification procedures are described

A flight investigation of a terminal area navigation and guidance concept for STOL aircraft

A digital avionics system referred to as STOLAND has been test-flown in the NASA CV-340 to obtain performance data for time-controlled guidance in the manual flight director mode. The advanced system components installed in the cockpit included an electronic attitude director indicator and an electronic multifunction display. Navigation guidance and control computations were all performed in the digital computer. Approach paths were flown which included a narrow 180-deg turn and a 1-min, 5-deg straight-in approach to the 30-m altitude go-around point. Results are presented for 20 approaches: (1) blended radio/inertial navigation using TACAN and a microwave scanning beam landing guidance system (MODILS) permitted a smooth transition from area navigation (TACAN) to precision terminal navigation (MODILS), (2) guidance system (flight director) performance measured at an altitude of 30.5 m was within that prescribed for category II CTOL operations on a standard runway, and (3) time of arrival at a point about 2 mi from touchdown was about 4 sec plus or minus sec later than the computed nominal arrival time

A flight investigation of a terminal area navigation and guidance concept for STOL aircraft

A digital avionics system was installed in the CV-340 transport aircraft. Flight tests were made to obtain preliminary performance data in the manual flight director mode using time controlled guidance. These tests provide a basis for selection of terminal area guidance, navigation, and control system concepts for short haul aircraft and for investigating operational procedures

High-Speed Rail Route and Regional Mobility with a Raster-Based Decision Support System: The Texas Urban Triangle Case

This study addresses sustainable transportation in the Texas Urban Triangle at the regional scale. Its aim is to determine the most suitable corridor for new transport infrastructure by employing a spatial decision support system proposed in this project. The system is being tested through its application to a prototype corridor parallel to Interstate 35 between San Antonio and Austin. The basic research questions asked are spatial in nature, so accordingly the geographic information system is the primary method of data analysis. The overall modeling approach is devoted to answering the following questions: What are the considerations to support sustainable growth? What scale or type of infrastructure is necessary? And how to adequately model the transportation corridors to meet the demands and to sustain the living environment at the same time

The Changing Geography of Outpatient Procedures

Since the early 80s, many surgical procedures have moved from the inpatient to outpatient setting. Outpatient surgical visits now account for about two-thirds of all surgical visits in the U.S. Over the same period, freestanding ambulatory surgery centers (ASCs) have arisen as alternatives to traditional hospital-based outpatient surgical departments. The number of ASCs grew from 240 in 1983 to 5,174 in 2008. The growth of ASCs raises safety concerns about the risk of complications and adequate access to emergency care. This Issue Brief summarizes evidence from one state about the changing geography of outpatient procedures and the possible risks associated with these changes