Common bean seeds treatment with Trichoderma for controlling Fusarium oxysporum f. sp. phaseoli.

The objective of this study was to evaluate Trichoderma harzianum isolates (CEN287, CEN288, CEN289, CEN290,CEN316) as biological control agents of F. oxysporum in seeds

Foliar application of Trichoderma for the control of white mold in common bean.

The objective of this study was to evaluate Trichoderma harzianum isolates (CEN287, CEN288, CEN289, CEN290 e CEN316), for the control of white mold of common bean cv. Perola, under field conditions.Pôster

Inhibition of CXCR2 Plays a Pivotal Role in Re-Sensitizing Ovarian Cancer to Cisplatin Treatment

cDNA microarray data conducted by our group revealed overexpression of CXCL2 and CXCL8 in ovarian cancer (OC) microenvironment. Herein, we have proven that the chemokine receptor, CXCR2, is a pivotal molecule in re-sensitizing OC to cisplatin, and its inhibition decreases cell proliferation, viability, tumor size in cisplatinresistant cells, as well as reversed the overexpression of mesenchymal epithelium transition markers. Altogether, our study indicates a central effect of CXCR2 in preventing tumor progression, due to acquisition of cisplatin chemoresistant phenotype by tumor cells, and patients’ high lethality rate. We found that the overexpression of CXCR2 by OC cells is persistent and anomalously confined to the cellular nuclei, thus pointing to an urge in developing highly lipophilic molecules that promptly permeate cells, bind to and inhibit nuclear CXCR2 to fight OC, instead of relying on the high-cost genetic engineered cells.acknowledge financial support from CAPES, FAPES and CNPq, as well as the Biotechnology Program/ RENORBIO from the Federal University of Espirito Santo, Espirito Santo, Brazil; Institute of Pathology and Molecular Immunology (IPATIMUP) and the Institute of Innovation and Health Research (I3s), Porto, Portugal

Avaliação do peso e escore de condição corporal de cabras leiteiras mantidas em pastagem de capim-tifton 85 sob lotação rotativa com diferentes estratégias de manejo.

Resumo: Avaliaram-se o peso e a condição corporal de cabras anglo-nubiano e saanen em lactação mantidas em pastagem de capim-tifton 85 manejada sob lotação rotativa ao longo do ano com diferentes estratégias de manejo. Os manejos consistiam em Convencional (altura residual 10 cm e sem adubação), Leve (altura residual 20 cm e sem adubação), Moderado (altura residual 20 cm e adubação equivalente a 300 kg de N/ha x ano) e Intensivo (altura residual 10 cm e adubação equivalente a 600 kg de N/ha x ano). Utilizou-se um delineamento inteiramente casualizado com medidas repetidas no tempo, sendo quatro repetições no período chuvoso e cinco no período seco sob irrigação por manejo. O peso dos animais ao longo do período chuvoso apresentou tendência linear decrescente, ao contrário do escore que apresentou pouca variação. Houve efeito dos manejos sobre o peso e o escore dos animais no período seco sob irrigação, com menor peso para os animais do manejo Intensivo. Houve grande oscilação do peso dos animais ao longo do período seco sob irrigação, assim como para o escore que apresentou ainda, uma crescente a partir do mês de dezembro. O peso e o escore de condição corporal são mecanismos do manejo nutricional que sofrem alterações em função de diferentes manejos da pastagem, sendo os animais do manejo Moderado os que mantiveram as melhores condições desses variáveis. [Weight and body condition score evaluation of the dairy goats kept in bermuda grass tifton 85 pasture under intermittent stocking with different management strategies]. Abstract: Was evaluated weight and body condition of anglo-nubiano and saanen lactating goats kept in Tifton 85 pastures managed under rotational stocking throughout the year with different management strategies. The management consisted of Conventional (residual height 10 cm and without fertilization), Lightweight (residual height 20 cm and without fertilization), Moderate (residual height 20 cm and fertilization equivalent to 300 kg N / ha x year) and Intensive (residual height 10 cm and fertilization equivalent to 600 kg N / ha x year). We used a completely randomized design with repeated measurements over time, with four replications in the rainy season and five in the dry season under irrigation management. The weight of the animals during the rainy season showed decreasing linear trend, unlike the score that showed little variation. There was effect of management upon the weight and the score of the animals in the dry season under irrigation, with less weight for animals Intensive management. There was wide variation in the proportion of animals throughout the dry season under irrigation as well as for the score that also had the increasingly from the month of December. The weight and body condition score are mechanisms of nutrient management that are altered due to different management of the pasture, and the handling of animals Moderate those who maintained the best conditions of these variables

Variations in Amazonian forest canopy structure and light environments across environmental and disturbance gradients.

A critical problem in tropical forest ecology is understanding how vegetation structure and function vary over environmental gradients. The degree to which forest structure changes across the Amazon basin and the role of environmental variability in shaping forest structure and dynamics are poorly characterised, despite the importance of these forests for regional and global climate. To address these challenges, we connected 10 years of investigations to amass a large database of ground-based profiling canopy lidar (PCL) data from 297 Amazon forest plots across large-scale environmental and disturbance gradients. Mean annual precipitation varied from 1,963 to 3,159 mm, number of dry season months from 0 to 5, and plot soil types covered about half of the variation in phosphorus, exchangeable cation, and soil physical property values observed in Amazonia. We quantified detailed metrics of vertical and horizontal structure and canopy light environments. Forest structure varied considerably across plots; maximum canopy height ranged from 6.1 to 35.7 m, gap fraction from 0.00 to 0.36, LAI from 0.5 to 7.3, rugosity from 1.5 to 7.5 m, and the relative height of 50% light transmission from 0.3 to 0.8. Disturbed sites exhibited almost twice the level of variation (SD) to non-disturbed sites for many metrics. Vertical leaf area density (LAD) profiles also showed high between plot variability, especially at low and high relative canopy heights. Plots with similar LAD profiles sometimes exhibited different distributions of ?canopy photic environment layers??where canopy leaf area is separated into photic environment layers by depth from canopy surface. This demonstrates that LAD profiles alone are insufficient for characterising canopy environments, essential to light-driven regeneration and carbon cycle processes. In addition, we evaluated relationships between lidar metrics and environmental variables extracted from geospatial layers. Our dataset allows a unique and detailed multi-site analysis of canopy structure and environments across the Amazon, including regions with little or no lidar sampling. Examining how structural attributes alter across environmental gradients is critical to understanding how current and future climate influences Amazonian forest structure, function, and dynamics.Paper 499657

Ciclo hidrológico e microclima.

Parte I - Caracterização física do Cerrado pé-de-gigante e uso das terras na região. Capítulo 4

Analogues of Marine Guanidine Alkaloids Are in Vitro Effective against Trypanosoma cruzi and Selectively Eliminate Leishmania (L.) infantum Intracellular Amastigotes

Synthetic analogues of marine sponge guanidine alkaloids showed in vitro antiparasitic activity against Leishmania (L.) infantum and Trypanosoma cruzi. Guanidines 10 and 11 presented the highest selectivity index when tested against Leishmania. The antiparasitic activity of 10 and 11 was investigated in host cells and in parasites. Both compounds induced depolarization of mitochondrial membrane potential, upregulation of reactive oxygen species levels, and increased plasma membrane permeability in Leishmania parasites. Immunomodulatory assays suggested an NO-independent effect of guanidines 10 and 11 on macrophages. The same compounds also promoted anti-inflammatory activity in L. (L.) infantum-infected macrophages cocultived with splenocytes, reducing the production of cytokines MCP-1 and IFN-γ. Guanidines 10 and 11 affect the bioenergetic metabolism of Leishmania, with selective elimination of parasites via a host-independent mechanism