Digital Reconstruction Supporting Investigation of Mishaps

In support of investigations of mishaps like the crash of the space shuttle Columbia, a process based on digital reconstruction from recovered components has been developed. The process is expected to reduce the need for physical reconstruction from recovered parts, reduce the time and cost of determining the cause of a mishap, and provide information useful in redesigning to prevent future mishaps. The process involves utilization of pre-existing techniques, hardware, and software to capture sizes and shapes of recovered parts in sets of digital data. The data are manipulated to enable rendering of captured geometric information by use of computer-aided design (CAD) and viewing software. The digitization of a part and study of its spatial relationship with other parts is taken to one of three levels of successively greater detail, depending on its importance to the investigation. The process includes a trajectory-analysis subprocess in which information from the digital reconstruction is combined with locations of recovered parts to reduce the area that must be searched to find other specified parts that have not yet been recovered. The digital product of the process is compatible with preexisting CAD and solid-model-rendering software

Pre-settlement Vegetation of Greene, Jersey and Macoupin Counties along the Prairie/Forest Border in Illinois

The US Federal Government established the Public Land Survey (PLS) to survey federal land before sale to settlers in the early 1800’s. We used a Geographic Information System (GIS) and the PLS to explore the relationship among forests, fire frequency, topography, and soils in Greene, Jersey and Macoupin Counties in Illinois. The PLS data was digitized from microfiche copies of the survey in the Lovejoy Library at Southern Illinois University Edwardsville. The floodplains along the Illinois River supported a fire-intolerant forest of Salix sp., Populus deltoides, Acer spp., and Fraxinus spp. The floodplain is bordered by a line of bluffs which forms a region of broken topography supporting moderately fire tolerant species on alfisols. To the East is a region of mixed prairie and forest. The prairie was on flat mollisols where it was intermixed with the forest on and hillier slopes and alfisols. The forest areas supported highly fire tolerant forest trees with large diameters at large distances from survey points. Non-Metric Multidimensional scaling reveals a close relationship among topography, vegetation and soils. Forests were on hilly land. Prairies were located on level uplands where there were no firebreaks. Floodplains are just as level as prairies. They are also located on mollisols adjacent to the river. They are protected from fire by sloughs, swamps and lakes. The results of our study support the conclusion that topography and moisture is an important control on the geographical distribution of vegetation in the study area. These factors likely operated by controlling fire frequency

Pre-settlement Vegetation of Greene, Jersey and Macoupin Counties along the Prairie/Forest Border in Illinois

The US Federal Government established the Public Land Survey (PLS) to survey federal land before sale to settlers in the early 1800’s. We used a Geographic Information System (GIS) and the PLS to explore the relationship among forests, fire frequency, topography, and soils in Greene, Jersey and Macoupin Counties in Illinois. The PLS data was digitized from microfiche copies of the survey in the Lovejoy Library at Southern Illinois University Edwardsville. The floodplains along the Illinois River supported a fire-intolerant forest of Salix sp., Populus deltoides, Acer spp., and Fraxinus spp. The floodplain is bordered by a line of bluffs which forms a region of broken topography supporting moderately fire tolerant species on alfisols. To the East is a region of mixed prairie and forest. The prairie was on flat mollisols where it was intermixed with the forest on and hillier slopes and alfisols. The forest areas supported highly fire tolerant forest trees with large diameters at large distances from survey points. Non-Metric Multidimensional scaling reveals a close relationship among topography, vegetation and soils. Forests were on hilly land. Prairies were located on level uplands where there were no firebreaks. Floodplains are just as level as prairies. They are also located on mollisols adjacent to the river. They are protected from fire by sloughs, swamps and lakes. The results of our study support the conclusion that topography and moisture is an important control on the geographical distribution of vegetation in the study area. These factors likely operated by controlling fire frequency

Efficacy of an Enhanced Implementation Strategy to Increase Parent Engagement with a Health Promotion Program in Childcare

Previous efforts to involve parents in implementation of childcare-based health promotion interventions have yielded limited success, suggesting a need for different implementation strategies. This study evaluated the efficacy of an enhanced implementation strategy to increase parent engagement with Healthy Me, Healthy We. This quasi-experimental study included childcare centers from the second of two waves of a cluster-randomized trial. The standard approach (giving parents intervention materials, prompting participation at home, inviting participation with classroom events) was delivered in 2016–2017 (29 centers, 116 providers, and 199 parents). The enhanced approach (standard plus seeking feedback, identifying and addressing barriers to parent participation) was delivered in 2017–2018 (13 centers, 57 providers, and 114 parents). Parent engagement was evaluated at two levels. For the center-level, structured interview questions with providers throughout the intervention were systematically scored. For the parent-level, parents completed surveys following the intervention. Differences in parent engagement were evaluated using linear regression (center-level) and mixed effects (parent-level) models. Statistical significance was set at p \u3c 0.025 for two primary outcomes. There was no difference in parent engagement between approaches at the center-level, β = −1.45 (95% confidence interval, −4.76 to 1.87), p = 0.38l. However, the enhanced approach had higher parent-level scores, β = 3.60, (95% confidence interval, 1.49 to 5.75), p \u3c 0.001. In the enhanced approach group, providers consistently reported greater satisfaction with the intervention than parents (p \u3c 0.001), yet their fidelity of implementing the enhanced approach was low (less than 20%). Results show promise that parent engagement with childcare-based health promotion innovations can positively respond to appropriately designed and executed implementation strategies, but strategies need to be feasible and acceptable for all stakeholders

Screen Time Parenting Practices and Associations with Preschool Children’s TV Viewing and Weight-Related Outcomes

The purpose of this study was to examine associations between screen time (ST) parenting practices and 2–5-year-old children’s TV viewing and weight status. Data were collected from 252 parent–child dyads enrolled in a randomized parent-focused childhood obesity prevention trial from 2009–2012. ST parenting practices were assessed at baseline using a validated parent-reported survey. Parent-reported child TV viewing and objectively measured anthropometrics were assessed at baseline, post-intervention (35 weeks), and follow-up (59 weeks). Marginal effect models were developed to test the association between baseline ST parenting practices and children’s TV viewing, BMI z-score, and waist circumference across all time points. Limiting/monitoring ST was associated with decreased weekly TV viewing (β = −1.79, 95% CI: −2.61; −0.95), while exposure to TV was associated with more weekly TV viewing over 59 weeks (β = 1.23, 95% CI: 0.71; 1.75). Greater parent use of ST as a reward was associated with increased child BMI z-score (β = 0.15, 95% CI: 0.03; 0.27), while limiting/monitoring ST was associated with decreased BMI z-score (β = −0.16, 95% CI: −0.30; −0.01) and smaller waist circumference (β = −0.55, 95% CI: −1.04; −0.06) over the study period. These findings suggest that modifying parent ST practices may be an important strategy to reduce ST and promote healthy weight in young children

efam: an expanded, metaproteome-supported HMM profile database of viral protein families

Motivation: Viruses infect, reprogram and kill microbes, leading to profound ecosystem consequences, from elemental cycling in oceans and soils to microbiome-modulated diseases in plants and animals. Although metagenomic datasets are increasingly available, identifying viruses in them is challenging due to poor representation and annotation of viral sequences in databases. Results: Here, we establish efam, an expanded collection of Hidden Markov Model (HMM) profiles that represent viral protein families conservatively identified from the Global Ocean Virome 2.0 dataset. This resulted in 240 311 HMM profiles, each with at least 2 protein sequences, making efam >7-fold larger than the next largest, panecosystem viral HMM profile database. Adjusting the criteria for viral contig confidence from 'conservative' to 'eXtremely Conservative' resulted in 37 841 HMM profiles in our efam-XC database. To assess the value of this resource, we integrated efam-XC into VirSorter viral discovery software to discover viruses from less-studied, ecologically distinct oxygen minimum zone (OMZ) marine habitats. This expanded database led to an increase in viruses recovered from every tested OMZ virome by similar to 24% on average (up to similar to 42%) and especially improved the recovery of often-missed shorter contigs (<5 kb). Additionally, to help elucidate lesser-known viral protein functions, we annotated the profiles using multiple databases from the DRAM pipeline and virion-associated metaproteomic data, which doubled the number of annotations obtainable by standard, single-database annotation approaches. Together, these marine resources (efam and efam-XC) are provided as searchable, compressed HMM databases that will be updated bi-annually to help maximize viral sequence discovery and study from any ecosystem

Evaluating the Effectiveness of an Autism-Specific Workplace Tool for Employers: A Randomised Controlled Trial

A randomised controlled trial evaluated the effectiveness of the Integrated Employment Success Tool (IEST™) in improving employers’ self-efficacy in modifying the workplace for individuals on the autism spectrum. Employers (N = 84) were randomised to the IEST™ or support as usual groups. Measurements of self-efficacy, knowledge and attitudes towards disability in the workplace were obtained at baseline and post-test. Results revealed a significant improvement in self-efficacy within the IEST™ group between baseline and post-test (p = 0.016). At post-test, there were no significant differences between groups in relation to self-efficacy in implementing autism-specific workplace modifications and employer attitudes towards disability in the workplace. Given the lack of significant outcomes, further research is needed to determine the effectiveness of the IEST™ for employers

Progress towards a public chemogenomic set for protein kinases and a call for contributions

Protein kinases are highly tractable targets for drug discovery. However, the biological function and therapeutic potential of the majority of the 500+ human protein kinases remains unknown. We have developed physical and virtual collections of small molecule inhibitors, which we call chemogenomic sets, that are designed to inhibit the catalytic function of almost half the human protein kinases. In this manuscript we share our progress towards generation of a comprehensive kinase chemogenomic set (KCGS), release kinome profiling data of a large inhibitor set (Published Kinase Inhibitor Set 2 (PKIS2)), and outline a process through which the community can openly collaborate to create a KCGS that probes the full complement of human protein kinases