106 research outputs found
A recoil detector for the measurement of antiproton-proton elastic scattering at angles close to 90
The design and construction of a recoil detector for the measurement of
recoil protons of antiproton-proton elastic scattering at scattering angles
close to 90 are described. The performance of the recoil detector has
been tested in the laboratory with radioactive sources and at COSY with proton
beams by measuring proton-proton elastic scattering. The results of laboratory
tests and commissioning with beam are presented. Excellent energy resolution
and proper working performance of the recoil detector validate the conceptual
design of the KOALA experiment at HESR to provide the cross section data needed
to achieve a precise luminosity determination at the PANDA experiment.Comment: 10 pages, 15 figure
An Enantioselective Artificial Suzukiase Based on the Biotin–Streptavidin Technology
Introduction of a biotinylated monophosphine palladium complex within streptavidin affords an enantioselective artificial Suzukiase. Site-directed mutagenesis allowed the optimization of the activity and the enantioselectivity of this artificial metalloenzyme. A variety of atropisomeric biaryls were produced in good yields and up to 90% ee. The hybrid catalyst described herein shows comparable TOF to the previous aqueous-asymmetric Suzuki catalysts, and excellent stability under the reaction conditions to realize higher TON through longer reaction time
Effect of different UCOE-promoter combinations in creation of engineered cell lines for the production of Factor VIII
<p>Abstract</p> <p>Background</p> <p>The most common approach used in generating cell lines for the production of therapetic proteins relies on gene amplification induced by a drug resistance gene e. g., DHFR and glutamine synthetase. Practically, this results in screening large number of clones for the one that expresses high levels of the biologic in a stable manner. The inefficiency of mammalian vector systems to express proteins in a stable manner typically involves silencing of the exogenous gene resulting from modifications such as methylation of CpG DNA sequences, histone deacetylation and chromatin condensation. The use of un-methylated CpG island fragments from housekeeping genes referred to as UCOE (ubiquitous chromatin opening elements) in plasmid vectors is now well established for increased stability of transgene expression. However, few UCOE-promoter combinations have been studied to date and in this report we have tested 14 different combinations.</p> <p>Findings</p> <p>In this report we describe studies with two different UCOEs (the 1.5 Kb human RNP fragment and the 3.2 Kb mouse RPS3 fragment) in combination with various promoters to express a large protein (B domain deleted factor VIII; BDD-FVIII) in a production cell line, BHK21. We show here that there are differences in expression of BDD-FVIII by the different UCOE-promoter combinations in both attached and serum free suspension adapted cells. In all cases, the 1.5 Kb human RNP UCOE performed better in expressing BDD-FVIII than their corresponding 3.2 Kb mouse RPS3 UCOE. Surprisingly, in certain scenarios described here, expression from a number of promoters was equivalent or higher than the commonly used and industry standard human CMV promoter.</p> <p>Conclusion</p> <p>This study indicates that certain UCOE-promoter combinations are better than others in expressing the BDD-FVIII protein in a stable manner in BHK21 cells. An empirical study such as this is required to determine the best combination of UCOE-promoter in a vector for a particular production cell line.</p
Machine studies for the development of storage cells at the ANKE facility of COSY
We present a measurement of the transverse intensity distributions of the
COSY proton beam at the target interaction point at ANKE at the injection
energy of 45 MeV, and after acceleration at 2.65 GeV. At 2.65 GeV, the machine
acceptance was determined as well. From the intensity distributions the beam
size is determined, and together with the measured machine acceptance, the
dimensions of a storage cell for the double-polarized experiments with the
polarized internal gas target at the ANKE spectrometer are specified. An
optimum storage cell for the ANKE experiments should have dimensions of 15mm x
20mm x 390mm (vertical x horizontal x longitudinal), whereby a luminosity of
about 2.5*10^29 cm^-2*s^-1 with beams of 10^10 particles stored in COSY could
be reached.Comment: 18 pages, 13 figures, 4 table
Measurement of the Spin-Dependence of the pbar-p Interaction at the AD-Ring
We propose to use an internal polarized hydrogen storage cell gas target in
the AD ring to determine for the first time the two total spin-dependent pbar-p
cross sections sigma_1 and sigma_2 at antiproton beam energies in the range
from 50 to 450 MeV. The data obtained are of interest by themselves for the
general theory of pbar-p interactions since they will provide a first
experimental constraint of the spin-spin dependence of the nucleon-antinucleon
potential in the energy range of interest. In addition, measurements of the
polarization buildup of stored antiprotons are required to define the optimum
parameters of a future, dedicated Antiproton Polarizer Ring (APR), intended to
feed a double-polarized asymmetric pbar-p collider with polarized antiprotons.
Such a machine has recently been proposed by the PAX collaboration for the new
Facility for Antiproton and Ion Research (FAIR) at GSI in Darmstadt, Germany.
The availability of an intense stored beam of polarized antiprotons will
provide access to a wealth of single- and double-spin observables, thereby
opening a new window on QCD spin physics.Comment: 51 pages, 23 figures, proposal submitted to the SPS committee of CER
Forward K+ production in subthreshold pA collisions at 1.0 GeV
K+ meson production in pA (A = C, Cu, Au) collisions has been studied using
the ANKE spectrometer at an internal target position of the COSY-Juelich
accelerator. The complete momentum spectrum of kaons emitted at forward angles,
theta < 12 degrees, has been measured for a beam energy of T(p)=1.0 GeV, far
below the free NN threshold of 1.58 GeV. The spectrum does not follow a thermal
distribution at low kaon momenta and the larger momenta reflect a high degree
of collectivity in the target nucleus.Comment: 4 pages, 3 figure
Untersuchungen zur Steigerung der Transkription durch CIS-agierende SAR-Elemente in Mammaliazellen
SIGLEAvailable from TIB Hannover: DW 7329 / FIZ - Fachinformationszzentrum Karlsruhe / TIB - Technische InformationsbibliothekDEGerman
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