Fistulas secondary to gynecological and obstetrical operations

The authors present urogenital and rectogenital fistulas treated at the Department of Obstetrics and Gynecology in Novi Sad in the period from 1976 to 1999. The study comprised 28 cases of fistula out of which 17 were vesicovaginal, 3 ureterovaginal, 1 vesicorecto vaginal and 7 recto vaginal. During the investigated period there were 182 Wertheim operations, 3864 total abdominal hysterectomies, 1160 vaginal hysterectomies and 7111 cesarean sections. The vesicovaginal fistulas were most frequent with the incidence of 0.33%, whereas the tocogenic fistulas did not occur. Urogenital fistulas secondary to radical hysterectomy are extremely rare thanks to the administered measures of prevention during the surgical procedure

Seasonal variability of Chelidonium majus L. secondary metabolites content and antioxidant activity

The aim of this study is to investigate the total phenolic content, concentration of flavonoids and antioxidant activity in extracts of the plant Chelidonium majus L. during different phenological stages (stage of rosette, the initial flowering stage, the stage of fully formed flowers and stage of fruits formation). Five different extracts of the whole plant, for each phase, were obtained by extraction with water, methanol, acetone, ethyl acetate and petroleum ether. The concentration of total phenolic content was determined using the Folin-Ciocalteu´s reagent and obtained values were the highest in the rosette stage (60.96 mg GA/g). The concentration of flavonoids was the highest in the initial stage of flowering (291.58 mg RU/g). The antioxidant activity was determined in vitro using DPPH reagent. The highest antioxidant activity was expressed in the rosette stage (50.72 mg/ml). Based on the obtained results it can be concluded that the concentrations of secondary metabolites in Ch. majus depend on the phenological stage of the plant

The availability of base elements (Ca, Mg, Na, K) in some important soil types in Serbia

In this paper results are presented of agrochemical and mineralogical analyses of 14 types of important soils in Serbia (Vojvodina and Central Serbia) and total content and availability of base alkali elements (Ca, Mg Na, K) are determined. Total element content was as follows: Ca 2.22%; K 1.77%; Na 0.85% and Mg 0.61%. Total content of alkali metals in the soils investigated and their variations within and between the soil types, is in very good/close correlation with contents of primary and secondary minerals as well as their rates of weathering. Taking in account the average availabilities the most abundant is calcium with 947 mg/100 g, whilst the averages of the other elements (Mg, K and Na) are quite similar and are about of 40 mg/100 g of soil. The results obtained have shown that the soils investigated are well to moderate provided with K, Ca and Mg and that their deficit could not be expected in plant nutrition, apart for some plants/cultures in the case of magnesium due to occasionally higher Ca/Mg and K/Mg ratios

The Potential Application of Selected Fungi Strains in Removal of Commercial Detergents and Biotechnology

The consumption of synthetic detergents is increasing year by year due to increasing urbanization, which reflects on higher concentration of this pollutant in the environment. In order to purify wastewaters from different pollutants, the application of new technologies such as bioremediation is necessary. From the environmental point of view, it is important to identify microorganisms that are tolerant to the presence of this pollutant. This chapter presents the experimental evaluation of ability of several fungal species, isolated from municipal sewage and industrial wastewater, in removing a high detergent concentration (anionic surfactants) from the environment as well as their potential application in biotechnology. The selected fungi were cultivated in Czapek-Dox liquid medium supplemented with commercial detergent “Merix” (Henkel, Kruševac, Serbia). Changes of physicochemical and biochemical parameters such as pH, redox potential, dry weight biomass, and enzymes activities such as alkaline protease and phosphatase were evaluated during 16 days of cultivation. The obtained results could be useful in the implementation of tested fungi in bioremediation processes and in biotechnology

Biochemical characteristics of selected species of fungi in the function of biodegradation detergent

Cilj ovog istraživanja bio je ispitivanje uticaja visokih koncentracija komercijalnog deterdženta „Merix“, (Henkel, Kruševac) na rast, razvoj i biohemijske karakteristike testiranih gljiva koje su izolovane iz kanalizacionih i industrijskih otpadnih voda. Gljive su gajene u tečnoj hranljivoj podlozi po Čapeku (kontrola) i istoj podlozi sa dodatkom deterdženta u koncentraciji 0,3% i 0,5%. U oglednom periodu od 3. do 16. dana ispitivane su promene: pH i redoks potencijala; količine proteina, slobodnih i ukupnih organskih kiselina; kvantitativni I kvalitativni sastav aminokiselina, kao i aktivnost određenih enzima u fermentacionoj tečnosti. Istovremeno je ispitivana i koncentracija deterdženta (anjonskih komponenti deterdženta) u fermentacionoj tečnosti pomoću MBAS metode. Sve gljive su razgradile deterdžent 0,3% koncentracije, a M. racemosus je razgradila i deterdžent 0,5% koncentracije. Deterdžent je uticao na inhibiciju biomase gljiva u različitom procentu u zavisnosti od vrste gljiva i mesta izolovanja. Kod gljive M. racemosus deterdžent je delovao stimulativno na biomasu, tako da je veća biomasa izmerena u podlozi sa 0,5% deterdžentom. Prisustvo deterdženta u hranljivoj podlozi i njegovih produkata degradacije tokom fermentacije gljiva uticalo je na promene svih ispitivanih biohemijskih parametara i enzimske aktivnosti.The aim of this study was to investigate the effect of high concentrations of commercial detergent "Merix" (Henkel, Krusevac) on growth and development and biochemical characteristics of the tested fungi were isolated from sewage and industrial wastewater. Fungi were grown in a liquid Czapek nutrient medium (control) and in the same medium with the addition of 0.3% and 0.5% detergent. The changing of pH values and redox potential; amounts of protein, free and total organic acids, the qualitative and quantitative composition of the amino acids, as well as the activity of certain enzymes were investigated in the fermentation liquid during the experimental period from 3 to 16 days. At the same time, concentration of detergent (anionic components of the detergent) in the fermentation broth was examined using MBAS method. All of the tested fungi were degrade detergent in concentration of 0.3%, but only M. racemosus decompose detergent in concentration of 0.5%. Detergent influenced the inhibition of the fungal biomass in varying percentages depending on the type of fungus and isolation. Detergent has a stimulating effect on biomass of fungus M. racemosus, so a greater biomass is measured in a medium containing 0.5% detergent. The presence of detergent in the nutrient medium and its degradation products during fermentation of fungi influenced the change of all the examined biochemical parameters and enzyme activities

Sadržaj fluora u zemljištima severnog Pomoravlja

Soil sampling was carried out in the Velika Morava river valley, covering the area from Velika Plana to the mouth of Morava to the Danube. The composite soil samples, representing alluvial soils (22 samples), cambisols (14) and smonitzas (4), were taken from plough layers, based on a regular square grid with intervals set at 5x5 km, covering total area of 100,000 ha. The total and available fluorine contents were determined in the soils samples. The highest average amount of total fluorine was found for alluvial soils (391 mg kg-1), then for smonitzas (348 mg kg-1) and the lowest one for cambisols (285 mg kg-1). These amounts are within normal fluorine content for soils (150-400 mg kg-1), although the maximum found levels were even about 500 mg kg-1. The available fluorine content was very low ( lt 1 mg kg-1), being mostly less than 0.2 % from its total amount, so it could be concluded that there was no danger from fluorine accumulation in the plants. Statistically significant correlation coefficient between total and available fluorine contents was not obtained. The total and available fluorine contents have mostly been in the correlation (with positive sign) with soil pH and the content of mechanical fraction silt+clay. Significant correlation coefficients between total fluorine content and the content of some heavy metals (Cr, Ni, Co, Cu, As) were also found, which indicated their mutual geochemical origin.U dolini reke Velike Morave, u delu od Velike Plane do njenog ušća u Dunav uzeti su reprezentativni uzorci zemljišta iz orničnog sloja, po mreži kvadrata veličine 5x5 km. U njima su odredjeni ukupni i pristupačni sadržaji fluora. Istraživanjem su obuhvaćena aluvijalna zemljišta (22 uzorka) gajnjače (14) i smonice (4), što odgovara površini od 100. 000 ha. Za ukupni fluor najviše srednje vrednosti su nadjene u aluvijumima (391 ppm), zatim u smonicama (348 ppm) i najmanje u gajnjačama (285 ppm). Ove vrednosti se nalaze u granicama normalnih sadržaja za zemljišta (150-400 ppm), mada se maksimalne vrednosti kreću i oko 500 ppm. Pristupačni sadržaj fluora je u ispitivanim zemljištima jako nizak ( lt 1 ppm), što najčešće čini ispod 0,2 % od ukupnog fluora, pa se može zaključiti da ne postoji opasnost od akumulacije fluora u biljkama koje se gaje na ispitivanom području. Nije nadjena korelativna veza ukupnog sa pristupačnim fluorom. Sadržaji ukupnog i pristupačnog fluora su najčešće bili u korelaciji (pozitivnoj) sa pH vrednošću zemljišta, i sadržajem frakcije prah+glina. Nadjene su i korelativne veze ukupnog fluora sa sadržajima nekih teških metala (Cr, Ni Co, Cu, As), što ukazije na njihovo zajedničko geohemijsko poreklo

Recognition of one class of quadrics from 3D point clouds

Within cyber physical production systems 3D vision as a source of information from real-world provides enormous possibilities. While the hardware of contemporary 3D scanners is characterized by high speed along with high resolution and accuracy, there is a lack of real-time online data processing algorithms that would give certain elements of intelligence to the sensory system. Critical elements of data processing software are efficient, real-time applicable methods for fully automatic recognition of high level geometric primitives from point cloud (surface segmentation and fitting). This paper presents a method for recognition of one class of quadrics from 3D point clouds, in particular for recognition of cylinders, elliptical cylinders and ellipsoids. The method is based on the properties of scatter matrix during direct least squares fitting of ellipsoids. Presented recognition procedure can be employed for segmentation of regions with G1 or higher continuity, and this is its comparative advantage to similar methods. The applicability of the method is illustrated and experimentally verified using two case studies. First case study refers to a synthesized, and the second to a real-world scanned point cloud

Recognition of one class of quadrics from 3D point clouds

Within cyber physical production systems 3D vision as a source of information from real-world provides enormous possibilities. While the hardware of contemporary 3D scanners is characterized by high speed along with high resolution and accuracy, there is a lack of real-time online data processing algorithms that would give certain elements of intelligence to the sensory system. Critical elements of data processing software are efficient, real-time applicable methods for fully automatic recognition of high level geometric primitives from point cloud (surface segmentation and fitting). This paper presents a method for recognition of one class of quadrics from 3D point clouds, in particular for recognition of cylinders, elliptical cylinders and ellipsoids. The method is based on the properties of scatter matrix during direct least squares fitting of ellipsoids. Presented recognition procedure can be employed for segmentation of regions with G1 or higher continuity, and this is its comparative advantage to similar methods. The applicability of the method is illustrated and experimentally verified using two case studies. First case study refers to a synthesized, and the second to a real-world scanned point cloud