Dynamics of spinning test particles in Kerr spacetime

We investigate the dynamics of relativistic spinning test particles in the spacetime of a rotating black hole using the Papapetrou equations. We use the method of Lyapunov exponents to determine whether the orbits exhibit sensitive dependence on initial conditions, a signature of chaos. In the case of maximally spinning equal-mass binaries (a limiting case that violates the test-particle approximation) we find unambiguous positive Lyapunov exponents that come in pairs ± lambda, a characteristic of Hamiltonian dynamical systems. We find no evidence for nonvanishing Lyapunov exponents for physically realistic spin parameters, which suggests that chaos may not manifest itself in the gravitational radiation of extreme mass-ratio binary black-hole inspirals (as detectable, for example, by LISA, the Laser Interferometer Space Antenna)

Bacterial Hsp70 resolves misfolded states and accelerates productive folding of a multi-domain protein

The ATP-dependent Hsp70 chaperones (DnaK in E. coli) mediate protein folding in cooperation with J proteins and nucleotide exchange factors (E. coli DnaJ and GrpE, respectively). The Hsp70 system prevents protein aggregation and increases folding yields. Whether it also enhances the rate of folding remains unclear. Here we show that DnaK/DnaJ/GrpE accelerate the folding of the multi-domain protein firefly luciferase (FLuc) 20-fold over the rate of spontaneous folding measured in the absence of aggregation. Analysis by single-pair FRET and hydrogen/deuterium exchange identified inter-domain misfolding as the cause of slow folding. DnaK binding expands the misfolded region and thereby resolves the kinetically-trapped intermediates, with folding occurring upon GrpE-mediated release. In each round of release DnaK commits a fraction of FLuc to fast folding, circumventing misfolding. We suggest that by resolving misfolding and accelerating productive folding, the bacterial Hsp70 system can maintain proteins in their native states under otherwise denaturing stress conditions. The Hsp70 system prevents protein aggregation and increases folding yields, but it is unknown whether it also enhances the rate of folding. Here the authors combine refolding assays, FRET and hydrogen/deuterium exchange-mass spectrometry measurements to study the folding of firefly luciferase and find that the bacterial Hsp70 actively promotes the folding of this multi-domain protein

The dynamics of precessing binary black holes using the post-Newtonian approximation

We investigate the (conservative) dynamics of binary black holes using the Hamiltonian formulation of the post-Newtonian (PN) equations of motion. The Hamiltonian we use includes spin-orbit coupling, spin-spin coupling, and mass monopole/spin-induced quadrupole interaction terms. In the case of both quasi-circular and eccentric orbits, we search for the presence of chaos (using the method of Lyapunov exponents) for a large variety of initial conditions. For quasi-circular orbits, we find no chaotic behavior for black holes with total mass 10 - 40 solar masses when initially at a separation corresponding to a Newtonian gravitational-wave frequency less than 150 Hz. Only for rather small initial radial distances, for which spin-spin induced oscillations in the radial separation are rather important, do we find chaotic solutions, and even then they are rare. Moreover, these chaotic quasi-circular orbits are of questionable astrophysical significance, since they originate from direct parametrization of the equations of motion rather than from widely separated binaries evolving to small separations under gravitational radiation reaction. In the case of highly eccentric orbits, which for ground-based interferometers are not astrophysically favored, we again find chaotic solutions, but only at pericenters so small that higher order PN corrections, especially higher spin PN corrections, should also be taken into account.Comment: 18 pages, 26 figure

Review

Molecular chaperones are highly conserved proteins that promote proper folding of other proteinsin vivo. Diverse chaperone systems assistde novoprotein folding and trafficking, the assembly of oligomeric complexes, and recovery from stress-induced unfolding. A fundamental function of molecular chaperones is to inhibit unproductive protein interactions by recognizing and protecting hydrophobic surfaces that are exposed during folding or following proteotoxic stress. Beyond this basic principle, it is now clear that chaperones can also actively and specifically accelerate folding reactions in an ATP-dependent manner. We focus on the bacterial Hsp70 and chaperonin systems as paradigms, and review recent work that has advanced our understanding of how these chaperones act as catalysts of protein folding

Novel Method to Process Cystic Fibrosis Sputum for Determination of Oxidative State

Background: Induced sputum is the most commonly used method to analyze airway inflammation in cystic fibrosis (CF) patients ex vivo. Due to the complex matrix of the sample material, precise and reliable analysis of sputum constituents depends critically on preanalytical issues. Objectives: Here we compared the commonly used method for sputum processing by dithiothreitol (DTT) with a novel mechanical method in regard to basal cellular parameters, neutrophil markers and glutathione (GSH) levels. Methods: Sputum samples from CF patients were processed in parallel with or without the use of DTT. The key improvement of the mechanical method was the processing in many very small aliquots. Cellular and humoral markers were assessed and compared according to Bland-Altman. Results: Total cell count, cell viability, differential cell count, neutrophil elastase levels and flow cytometrically analyzed neutrophil markers (CD63, CD11b, DHR) did not differ between the two methods. Intracellular and extracellular GSH levels were significantly higher in DTT-treated samples (p = 0.002). Conclusion: The mechanical sputum-processing method presented had a similar yield of cells and fluids as the conventional DTT method and the advantage of omitting the introduction of reducing agents. This method allows a more reliable analysis of redox-dependent airway inflammation in sputum cells and fluid from CF patients than methods utilizing DTT. Copyright (C) 2009 S. Karger AG, Base