12 research outputs found
Podosome rings generate forces that drive saltatory osteoclast migration
Podosomes are dynamic, actin-containing adhesion structures that self-organize as rings in osteoclasts. We observe that podosome rings generate tension forces and that osteoclasts migrate by periodically moving toward the actin-containing structures. Actin dynamics thus not only correlate with the cell migration, but drive it
Cytoplasmic hnRNPK interacts with GSK3β and is essential for the osteoclast differentiation
Potassium citrate prevents increased osteoclastogenesis resulting from acidic conditions: Implication for the treatment of postmenopausal bone loss - Fig 3
<p><b>A)</b> RANKL-mediated OC differentiation in acidic and neutral microenvironment. The bars represent the mean ± SEM of the total number of OC/cm<sup>2</sup> obtained from buffy-coat of four individuals and cultured at pH 7.4 and pH 6.9 with three different RANKL concentrations. Symbols (*) indicate statistically significant differences vs RANKL 50 ng/mL at pH 7.4. <b>B)</b> The representative pictures show that the size of TRAcP positive multinucleated cells and the differentiating potency of low RANKL concentrations are higher at pH 6.9 than at pH 7.4. Magnification x 10, Scale bar = 50 μm. <b>(C)</b> and <b>(D)</b> K citrate inhibits the OC differentiation induced at pH 6.9 by high (C: 50 ng/mL) and low (D: 0.5 ng/mL) RANKL doses. Results are expressed as a ratio between the number of OC counted in cultures treated with different K citrate concentrations and number of OC counted in the negative control (K citrate 0 mM, ratio = 1). The positive control is Alendronate 10<sup>-5</sup>M. Mean ± SEM of data obtained from three different donors and two replicates for each condition. Symbols (*) indicate statistically significant differences vs negative control (pH 6.9, K citrate 0 mM, ratio = 1). In all histograms P values ≤ 0.05, ≤ 0.01 and ≤ 0.001 were highlighted by one, two or three symbols, respectively.</p