Biochemical properties of Paracoccus denitrificans FnrP:Reactions with molecular oxygen and nitric oxide

In Paracoccus denitrificans, three CRP/FNR family regulatory proteins, NarR, NnrR and FnrP, control the switch between aerobic and anaerobic (denitrification) respiration. FnrP is a [4Fe-4S] cluster containing homologue of the archetypal O2 sensor FNR from E. coli and accordingly regulates genes encoding aerobic and anaerobic respiratory enzymes in response to O2, and also NO, availability. Here we show that FnrP undergoes O2-driven [4Fe-4S] to [2Fe-2S] cluster conversion that involves up to 2 O2 per cluster, with significant oxidation of released cluster sulfide to sulfane observed at higher O2 concentrations. The rate of the cluster reaction was found to be ~6-fold lower than that of E. coli FNR, suggesting that FnrP can remain transcriptionally active under microaerobic conditions. This is consistent with a role for FnrP in activating expression of the high O2 affinity cytochrome c oxidase under microaerobic conditions. Cluster conversion resulted in dissociation of the transcriptionally active FnrP dimer into monomers. Therefore, along with E. coli FNR, FnrP belongs to the subset of FNR proteins in which cluster type is correlated with association state. Interestingly, two key charged residues, Arg140 and Asp154, that have been shown to play key roles in the monomer-dimer equilibrium in E. coli FNR are not conserved in FnrP, indicating that different protomer interactions are important for this equilibrium. Finally, the FnrP [4Fe-4S] cluster is shown to undergo reaction with multiple NO molecules, resulting in iron nitrosyl species and dissociation into monomers

DIVERGENT REACTIONS OF ESTERS OF ACYLPYRUVIC ACIDS WITH o-PHENYLENEDIAMINE

This work was supported by the Ministry of Science and Higher Education of the Russian Federation (FSNF-2020-0008), Russian Foundation for Basic Research (project no. 20-43-596008) and the Government of Perm Krai. Antimicrobial screening was performed by CO-ADD (The Community for Antimicrobial Drug Discovery), funded by the Wellcome Trust (UK) and The University of Queensland (Australia)

Infeksion endokarditin klinik-morfoloji təzahürləri: klinik praktikadan məlumat

Проведен анализ собственного клинико-морфологического наблюдения инфекционного эндокардита, особенностью которого следует считать нетипичную манифестацию заболевания, проявившуюся субарахноидальным кровоизлиянием вследствие иммуномолекулярных и иммуноклеточных повреждений стенок сосудов головного мозга, при отсутствии внешних типичных проявлений и стёртой кардиальной симптоматики, что в свою очередь затруднило своевременное установление правильного клинического диагноза и привело к смерти больного. Неспецифическое проявление заболевания, которое осложнилось субарахноидальным кровоизлиянием, препятствовало своевременному установлению правильного клинического диагноза, это следует рассматривать как особенность зарегистрированного случая; Məqalədə infeksion endokarditin etiologiyası, patogenezi, patomorfoloji təsnifatı və onun yaranmasına meyl törədən amillər haqqında müasir ədəbiyyat məlumatları əks etdirilmiş və müəlliflərin öz klinik praktikalarında rast gəldikləri bir xəstə haqqında məlumat verilmişdir. Göstərilir ki, infeksion endokarditli xəstədə xəstəliyin atipik təzahürü kimi, subaraxnoidal qansızma inkişaf etmişdir. Müəlliflər bu ağırlaşmanın səbəbini baş beyninin damarlarının immunmolekulyar və immun-hüceyrəvi mexanizmlərlə zədələnməsi ilə izah edirlər. Göstərilir ki, xəstədə aydın kardial simptomatikanın olmaması xəstəliyə vaxtında diaqnoz qoyulmasını çətinləşdirmiş və bu da xəstənin ölümünə səbəb olmuşdur. Xəstəliyin qeyri-spesifik əlamətləri subaraxnoidal qansızma sayəsində arxa plana keçdiyindən, düzgün klinik diaqnoz qoyulmasına mane olmuşdur ki, bu da təsvir edilən klinik müşahidənin əsas xüsusiyyətini təşkil edir

STATE OF SEED-GROWING AND VARIETY CONTENT OF WINTER WHEAT IN THE ROSTOV REGION

During a long reproduction any variety tends to decrease its economic-biological traits and properties, which it initially possesses. The reasons for this are different diseases, especially infectious, mechanical and biological littering, which results in decreasing of productivity and grain quality of winter wheat. To overcome it we have to carry out a variety upgrade (renewal), e.i. replacement of the low productive seeds with highly productive ones possessing good sowing features.  In the Rostov region winter wheat is one of the basic grain crops. During last 14 years its area varied from 813,1 (in 2000) till 2096,5 thousand ha (in 2014). The maximum productivity was 36,7 c/ha in 2004. The work gives the average data about the amount of sown original, basic and reproductive seeds of winter wheat in the Rostov region during 2012-2014 years. 38.300 tons of original and basic seeds of winter wheat were sown in 2012, 26.500 t in 2013 and 37.700 t in 2014. Nevertheless, we should note the fact that the most part of the area was sown with the seeds of 1-4 reproduction. We have been analyzing the variety content of winter wheat in different parts of the Rostov region. The main breeding institutions-originators which produce winter wheat varieties for the Rostov region in 2014 are ARRIGC (38,4%), DRIA (28,6%) and KRIA (27,8%), the seeds of which were sown on 94,8% of the area. The varieties of SRIA and SSI occupied 4,6% of the area, the rest (0,6%) belonged to the cultivars of the other institutions