Antioxidants, radical-scavenging and protein carbonylation inhibition capacity of six monocultivar virgin olive oils in Istria (Croatia)

Six monocultivar virgin olive oils (VOOs) produced from five autochthonous (Buža, Buža Puntoža, Istarska Bjelica, Porečka Rosulja, and Rosinjola) and one referent cultivar (Leccino), were investigated. The mass fractions of orthodiphenols (ORT) and tocopherols (TOC) were analysed by VIS spectroscopy and HPLC. The radical-scavenging capacity was evaluated by electron spin resonance spectroscopy (ESR) using galvinoxyl free radical and the DPPH test. Results revealed a high level of total TOC in VOOs of Buža Puntoža (243 mg kg−1) and Porečka Rosulja (325 mg kg−1). VOOs contained in 100 g from 79% (Istarska Bjelica) to 261% (Porečka Rosulja) of recommended daily allowance of α-tocopherol. The mass fraction of ORT in Istarska Bjelica and Rosinjola was >250 mg kg−1 (on average 30–40% higher in comparison to other studied cultivars). Istarska Bjelica and Porečka Rosulja showed the highest antioxidant capacity in both methods of measurement. The highest capacity to inhibit protein carbonylation (PC) in response to oxidative stress (54–56%) was displayed by Buža Puntoža, Leccino, and Buža. High level of positive linear correlation between ORT mass fraction and radical-scavenging capacity measured by DPPH test (r=0.768), as well as strong negative correlation between PC inhibition and mass fraction of ORT (r= −0.697), were observed

Nutritive characteristics of probiotic quark as influenced by type of starter

Quark was manufactured, under laboratory conditions, by standard batch process, from two types of milk: I) partially skimmed, with 1.6 % of fat and II) whole milk, with 3.5 % of fat. As starters, two probiotic cultures were applied: I) DVS-Probio-TecTM ABT- 1, Lactobacillus acidophilus-5, Bifidobacterium-12, S. thermophilus and II) DVS-Probio-TecTM ABT-2, Lactobacillus acidophilus-5, Bifidobacterium-12, S. thermophilus. Also, the traditional cheese culture (CH - N22) was used. The obtained Quark samples were tested by standard methods in order to determine their nutritive characteristics. The results have shown that probiotic starter ABT-1 ensured the highest level of utilization of fat, proteins, lactose and phosphorus from partially skimmed milk. Probiotic culture ABT-2 was less effective, but combined with the traditional starter and applied to a whole milk could give Quark with acceptable nutritive characteristics. When sensory characteristics of products were tested, it has been proven that the application of ABT-2 culture and combination of probiotic starters ABT-2 and traditional culture gave good products with typical mild flavor. On the contrary, inoculation with ABT-1 probiotic starter resulted in lower-quality products in case of both kinds of milk applied.

Industrially produced enriched sterilized milk

Nepravilna ishrana bogata mastima i ugljenim hidratima, a siromašna u biološki visokovrijednim sastojcima hrane prisutna je još uvijek u našoj zemlji. Ova ispitivanja su izvedena sa ciljem dobivanja zdrave hrane na bazi mlijeka, obogaćene proteinima, vitaminima i mineralnim tvarima. U industrijskim uvjetima su uspješno proizvedena sterilizirana mlijeka obogaćena Na-kazeinatom, vitaminom A, D i željezom. Fizičko-kemijska, mikrobiološka i organoleptička kvaliteta proizvoda je kontrolirana tokom tromjesečnog skladištenja.The irregular nutrition, rich in fats and carbohydrates, and poor in biologically highly valuable food components, is still present in our country. These investigations were carried out in the aim of obtaining dairy based healthy foods enriched with proteins, vitamins and minerals. Sterilized milks enriched with Na-caseinate, vitamin AD and iron, were successfully produced under industrial conditions. Physico-chemical, microbiological and organoleptical qualities of the products were controlled during three months storage

Determination of biological activity of protein component in milk, modified for infant feeding, by pepsin-pancreatin digest index

Kao alternativa humanom mlijeku, u slučajevima kada majka ne može da hrani dijete vlastitim mlijekom, danas se u svijetu koristi najčešće kravlje mlijeko čiji je sastav modificiran da bi se približio sastavu humanog mlijeka. Kravlje mlijeko ima 3,5 puta više proteina od humanog mlijeka; osim toga u sastavu proteina kravljeg mlijeka nalazi se 79% kazeina i 21% serum proteinu, a u proteinima humanog mlijeka 35% kazeina i 65% serum proteina. Sastav serum proteina također nije identičan. U skladu sa ovim razlikuje se i amino-kiselinski sastav navedenih proteina i njihova biološka vrijednost. Cilj ovog rada bio je ispitivanje biološke aktivnosti modificiranih proteina u smjeru kazein-serum protein u proizvodnji "infant formula" pepsin-pankreatin testom. Ustanovljeno je utoliko veće povećanje aktivnosti ukoliko je sustav bliži proteinu humanog mlijeka.This study was carried out with the aim to determine biological activity of milk proteins, modified to supstitute human milk proteins in infant formulae, by pepsin-pancreatin digest index. The better biological activity was obtained by samples with higher simmilarity of modified milk proteins to human milk proteins

Determination of biological activity of protein component in milk, modified for infant feeding, by pepsin-pancreatin digest index

Kao alternativa humanom mlijeku, u slučajevima kada majka ne može da hrani dijete vlastitim mlijekom, danas se u svijetu koristi najčešće kravlje mlijeko čiji je sastav modificiran da bi se približio sastavu humanog mlijeka. Kravlje mlijeko ima 3,5 puta više proteina od humanog mlijeka; osim toga u sastavu proteina kravljeg mlijeka nalazi se 79% kazeina i 21% serum proteinu, a u proteinima humanog mlijeka 35% kazeina i 65% serum proteina. Sastav serum proteina također nije identičan. U skladu sa ovim razlikuje se i amino-kiselinski sastav navedenih proteina i njihova biološka vrijednost. Cilj ovog rada bio je ispitivanje biološke aktivnosti modificiranih proteina u smjeru kazein-serum protein u proizvodnji "infant formula" pepsin-pankreatin testom. Ustanovljeno je utoliko veće povećanje aktivnosti ukoliko je sustav bliži proteinu humanog mlijeka.This study was carried out with the aim to determine biological activity of milk proteins, modified to supstitute human milk proteins in infant formulae, by pepsin-pancreatin digest index. The better biological activity was obtained by samples with higher simmilarity of modified milk proteins to human milk proteins

The Relevance of the Territorial Dimension in EU Policies and the Role of Tourism

The disparities, inequalities as well as the different trajectories of social and economic development that still characterize the regions of the EU today demonstrate the importance of a debate on the spatial dimension of European policies. This paper analyses the implications of strengthening the spatial dimension in the different EU programs, highlighting the importance of the concept of territorial cohesion and the role that a strategic sector such as tourism can play in implementing this concept. Therefore, the added value of tourism and the potential of territorial cohesion should play an important role in future European policies and fundin