Effects of nanoparticles on murine macrophages

Metallic nanoparticles are more and more widely used in an increasing number of applications. Consequently, they are more and more present in the environment, and the risk that they may represent for human health must be evaluated. This requires to increase our knowledge of the cellular responses to nanoparticles. In this context, macrophages appear as an attractive system. They play a major role in eliminating foreign matter, e.g. pathogens or infectious agents, by phagocytosis and inflammatory responses, and are thus highly likely to react to nanoparticles. We have decided to study their responses to nanoparticles by a combination of classical and wide-scope approaches such as proteomics. The long term goal of this study is the better understanding of the responses of macrophages to nanoparticles, and thus to help to assess their possible impact on human health. We chose as a model system bone marrow-derived macrophages and studied the effect of commonly used nanoparticles such as TiO2 and Cu. Classical responses of macrophage were characterized and proteomic approaches based on 2D gels of whole cell extracts were used. Preliminary proteomic data resulting from whole cell extracts showed different effects for TiO2-NPs and Cu-NPs. Modifications of the expression of several proteins involved in different pathways such as, for example, signal transduction, endosome-lysosome pathway, Krebs cycle, oxidative stress response have been underscored. These first results validate our proteomics approach and open a new wide field of investigation for NPs impact on macrophagesComment: Nanosafe2010: International Conference on Safe Production and Use of Nanomaterials 16-18 November 2010, Grenoble, France, Grenoble : France (2010

The use of misoprostol in outpatient treatment of abortions in the first quarter of pregnancy in Dakar

Background: The objective of this study was to compare the effectiveness of misoprostol comparing to MVA in support of abortion from the first quarter.Methods: This was a prospective, descriptive and analytical study (case-control study) made between January 1st and December 31st, 2015 in a level 1 maternity in Dakar.Results: The study included316 first trimester abortions (158 were treated with misoprostol and 158 with MVA). The epidemiological profile was a woman aged on average 28.5 years and nulliparous (37%). The mean gestational age was 8 weeks 6 days. The rate of complete uterine evacuation was comparable in both groups (93% for misoprostol versus 94.3%for MVA) with a non-significant p-value. Side effects found (40.8%) were minor. The hospital stay averaged two hours in the misoprostol group against 24 hours for MVA. Anaemia was more common in the MVA group (44.1%) than in the misoprostol group (23.6%) (p = 0.0006). The cost of treatment with misoprostol (5620 CFA francs) was on average four times less than that of MVA (21,623 CFA francs).Conclusions: Misoprostol can be seen as a credible alternative in the management of first quarter abortions because of its many advantages including its effectiveness, its easiness to be used in ambulatory, its low cost and safety

Gyrokinetic analysis and simulation of pedestals, to identify the culprits for energy losses using fingerprints

Fusion performance in tokamaks hinges critically on the efficacy of the Edge Transport Barrier (ETB) at suppressing energy losses. The new concept of fingerprints is introduced to identify the instabilities that cause the transport losses in the ETB of many of today's experiments, from widely posited candidates. Analysis of the Gyrokinetic-Maxwell equations, and gyrokinetic simulations of experiments, find that each mode type produces characteristic ratios of transport in the various channels: density, heat and impurities. This, together with experimental observations of transport in some channel, or, of the relative size of the driving sources of channels, can identify or determine the dominant modes causing energy transport. In multiple ELMy H-mode cases that are examined, these fingerprints indicate that MHD-like modes are apparently not the dominant agent of energy transport; rather, this role is played by Micro-Tearing Modes (MTM) and Electron Temperature Gradient (ETG) modes, and in addition, possibly Ion Temperature Gradient (ITG)/Trapped Electron Modes (ITG/TEM) on JET. MHD-like modes may dominate the electron particle losses. Fluctuation frequency can also be an important means of identification, and is often closely related to the transport fingerprint. The analytical arguments unify and explain previously disparate experimental observations on multiple devices, including DIII-D, JET and ASDEX-U, and detailed simulations of two DIII-D ETBs also demonstrate and corroborate this

Acute complications of preeclampsia: prognosis and management at Pikine National Hospital in Dakar (Senegal)

ABSTRACTBackground: The objective of this study was to evaluate the prevalence of acute complications of preeclampsia in order to describe the epidemiological profile of the disease, to assess its prognosis and management.Methods: This was a retrospective study of patients admitted to the Pikine National Hospital from 1 January 2010 to 31 December 2013 (48 months) with severe complicated pre-eclampsia. Included in this study were patients admitted or diagnosed with severe complicated pre-eclampsia and having given birth in the structure or not.Results: The incidence of severe preeclampsia in childbirth varied from 9.7% to 11.5% during the four years of our study. Patients were largely paucigest (55.7% of cases) and paucipares (58.5% of cases). The mean age was 28.14 years with extremes of 14 and 47 years. More than half of the patients (57.7%) were between 21 and 34 years of age. They were mostly married (90.7%). Three-quarters of the patients (76.8%) had proteinuria with ≥ 3 cross-bands. Thrombocytopenia was found in 9.7% of patients, hepatic cytolysis in 12.1%, and elevation of serum creatinine in 13.8%. The level of transaminases was found to be greater than 2 in the normal range in 12.1%. Complicated forms were the most represented in our study. These were acute complications, with 715 cases, or 57.3% of the patients. They were either isolated (52.8%) or associated (4.5%). These included eclampsia (24.9%), followed by retroplacental hematoma (24.6%), fetal death in utero (23.7%), HELLP syndrome (3.4%). , Acute edema of the lungs (1.5%), and acute renal failure (1.4%). The lethality was 2.4%. The causes of maternal death were dominated by eclampsia (14 cases), DIC (3 cases) and OAP (2 cases). We counted 77.7% of live births and a stillbirth of 254.5 ‰.Conclusions: Pre-eclampsia is a serious complication of pregnancy. Its frequency is still high in sub-Saharan Africa. In the presence of signs of severity, maternal (vital and functional) and neonatal prognosis are inevitably involved. If management is based on fetal extraction, resuscitation measures are a guarantee of maternal survival

Enhancement of vaccinia virus based oncolysis with histone deacetylase inhibitors

Histone deacetylase inhibitors (HDI) dampen cellular innate immune response by decreasing interferon production and have been shown to increase the growth of vesicular stomatitis virus and HSV. As attenuated tumour-selective oncolytic vaccinia viruses (VV) are already undergoing clinical evaluation, the goal of this study is to determine whether HDI can also enhance the potency of these poxviruses in infection-resistant cancer cell lines. Multiple HDIs were tested and Trichostatin A (TSA) was found to potently enhance the spread and replication of a tumour selective vaccinia virus in several infection-resistant cancer cell lines. TSA significantly decreased the number of lung metastases in a syngeneic B16F10LacZ lung metastasis model yet did not increase the replication of vaccinia in normal tissues. The combination of TSA and VV increased survival of mice harbouring human HCT116 colon tumour xenografts as compared to mice treated with either agent alone. We conclude that TSA can selectively and effectively enhance the replication and spread of oncolytic vaccinia virus in cancer cells. © 2010 MacTavish et al

Genetic Diversification and Selection Strategies for Improving Sorghum Grain Yield Under Phosphorous-Deficient Conditions in West Africa

Sorghum, a major crop for income generation and food security in West and Central Africa, is predominantly grown in low-input farming systems with serious soil phosphorus (P) deficiencies. This study (a) estimates genetic parameters needed to design selection protocols that optimize genetic gains for yield under low-phosphorus conditions and (b) examines the utility of introgressed backcross nested association mapping (BCNAM) populations for diversifying Malian breeding materials. A total of 1083 BC1F5 progenies derived from an elite hybrid restorer “Lata-3” and 13 diverse donor accessions were evaluated for yield and agronomic traits under contrasting soil P conditions in Mali in 2013. A subset of 298 progenies were further tested under low-P (LP) and high-P (HP) conditions in 2014 and 2015. Significant genetic variation for grain yield was observed under LP and HP conditions. Selection for grain yield under LP conditions was feasible and more efficient than the indirect selection under HP in all three years of testing. Several of the BCNAM populations exhibited yields under LP conditions that were superior to the elite restorer line used as a recurrent parent. The BCNAM approach appears promising for diversifying the male parent pool with introgression of diverse materials using both adapted Malian breed and unadapted landrace material from distant geographic origins as donors

Incidence et regulation naturelle de la chenille mineuse de l’epi de mil, Heliocheilus albipunctella de joannis (Lepidoptera, Noctuidae) a bambey dans le bassin arachidier au Senegal

Au Sénégal, la mineuse de l’épi, Heliocheilus albipunctella (Lepidoptera, Noctuidae) a commencé à causer des dégâts dans les cultures de mil suite à une longue période de sécheresse au début des années 70. Le suivi d’un réseau de 45 parcelles de producteurs à Bambey en 2013, a permis d’évaluer la situation du ravageur : abondance relative (oeuf et larve), parasitisme associé, potentiel de régulation naturelle et pertes de rendement liées aux dégâts. Les résultats ont montré une distribution très hétérogène du ravageur dans la zone avec des moyennes d’infestation des épis en oeufs de 40 % et en larves 76 %. Un taux moyen de parasitisme des oeufs par Trichogrammatoïdea sp estimé à 2 % est noté (n = 2281 oeufs). Le parasitisme larvaire est dominé par des cocons d’endoparasitoïdes de la famille des Ichneumonidae (8,6 %), des larves de Tachinidae (5,2 %) et des morphotypes non encore identifiés (4,6 %). Une faible mortalité larvaire due au Bracon sp. (1,5 %, n = 1567 larves) est observée. Ce faible taux de parasitisme, comparé au potentiel réel de la régulation naturelle observée (59 %, n = 45 parcelles) montre l’importance probable des ennemis naturels dans le contrôle du ravageur. Les pertes en grains sont estimées à 10 %.Mots clés : Heliocheilus albipunctella, mil, régulation écologique, ennemis naturels, dégât

Magnetic Excitations of Undoped Iron Oxypnictides

We study the magnetic excitations of undoped iron oxypnictides using a three-dimensional Heisenberg model with single-ion anisotropy. Analytic forms of the spin wave dispersion, velocities, and structure factor are given. Aside from quantitative comparisons which can be made to inelastic neutron scattering experiments, we also give qualitative criteria which can distinguish various regimes of coupling strength. The magnetization reduction due to quantum zero point fluctuations shows clear dependence on the c-axis coupling.Comment: 4 pages, 5 figures, to appear in Frontiers of Physics in China: a special issue on Iron-based superconductor