Investigation of grain separation through straw layer over …

Straw walker is a separator that separates grain from straw, and limits the efficiency of combine-harvesters therefore special attention is devoted to the development and improvement of its design and technological parameters. The most important parameter of walker operation is Froude-number k that depends on the amplitude of the straw walker movement in vertical direction, i.e., walker sieve oscillation amplitude r and angular velocity ω. High speed camera used in the investigation tests helped to determine not only the behavior of the straw layers on the oscillating sieve of straw walker but also the duration of grain penetration (separation) through the straw. With estimation of these parameters the rational values of Froude-number k were substantiated. It has been determined that at various ω and r combinations when k=const. sieve vertical accelerations when the crankshaft is rotated at the same angle are equal, but speeds and displacements are varied. For this reason the duration of grain separation is different. The rational angular velocity of the crankshaft is 22.5 s-1 (r=0.05 m), as then the straw layer when the crankshaft is rotated at the angle 2π, raised from the walker surface (sieve) the most early and strokes with it latest, i.e. the duration of free movement of the straw is 1.5 times longer and the grain separation about 15% more intensive than when ω=21.5 s-1. The estimation of grain separation enabled to define critical value of crankshaft angular velocity equal to ω=23.5 s-1. When this value is exceeded the top straw layers receive only one stroke when the crankshaft is rotated at the angle 4π, therefore the increase of crankshaft angular velocity and, simultaneously, sieve oscillation intensity above the critical value is inexpedient

Full characterization of vibrational coherence in a porphyrin chromophore by two-dimensional electronic spectroscopy

In this work we present experimental and calculated two-dimensional electronic spectra for a 5,15-bisalkynyl porphyrin chromophore. The lowest energy electronic Qy transition couples mainly to a single 380 cm–1 vibrational mode. The two-dimensional electronic spectra reveal diagonal and cross peaks which oscillate as a function of population time. We analyze both the amplitude and phase distribution of this main vibronic transition as a function of excitation and detection frequencies. Even though Feynman diagrams provide a good indication of where the amplitude of the oscillating components are located in the excitation-detection plane, other factors also affect this distribution. Specifically, the oscillation corresponding to each Feynman diagram is expected to have a phase that is a function of excitation and detection frequencies. Therefore, the overall phase of the experimentally observed oscillation will reflect this phase dependence. Another consequence is that the overall oscillation amplitude can show interference patterns resulting from overlapping contributions from neighboring Feynman diagrams. These observations are consistently reproduced through simulations based on third order perturbation theory coupled to a spectral density described by a Brownian oscillator model

Investigation of grain separation through straw layer over …

Straw walker is a separator that separates grain from straw, and limits the efficiency of combine-harvesters therefore special attention is devoted to the development and improvement of its design and technological parameters. The most important parameter of walker operation is Froude-number k that depends on the amplitude of the straw walker movement in vertical direction, i.e., walker sieve oscillation amplitude r and angular velocity ω. High speed camera used in the investigation tests helped to determine not only the behavior of the straw layers on the oscillating sieve of straw walker but also the duration of grain penetration (separation) through the straw. With estimation of these parameters the rational values of Froude-number k were substantiated. It has been determined that at various ω and r combinations when k=const. sieve vertical accelerations when the crankshaft is rotated at the same angle are equal, but speeds and displacements are varied. For this reason the duration of grain separation is different. The rational angular velocity of the crankshaft is 22.5 s-1 (r=0.05 m), as then the straw layer when the crankshaft is rotated at the angle 2π, raised from the walker surface (sieve) the most early and strokes with it latest, i.e. the duration of free movement of the straw is 1.5 times longer and the grain separation about 15% more intensive than when ω=21.5 s-1. The estimation of grain separation enabled to define critical value of crankshaft angular velocity equal to ω=23.5 s-1. When this value is exceeded the top straw layers receive only one stroke when the crankshaft is rotated at the angle 4π, therefore the increase of crankshaft angular velocity and, simultaneously, sieve oscillation intensity above the critical value is inexpedient

Geometry-dependent electrostatics near contact lines

Long-ranged electrostatic interactions in electrolytes modify their contact angles on charged substrates in a scale and geometry dependent manner. For angles measured at scales smaller than the typical Debye screening length, the wetting geometry near the contact line must be explicitly considered. Using variational and asymptotic methods, we derive new transcendental equations for the contact angle that depend on the electrostatic potential only at the three phase contact line. Analytic expressions are found in certain limits and compared with predictions for contact angles measured with lower resolution. An estimate for electrostatic contributions to {\it line} tension is also given.Comment: 3 .eps figures, 5p

Inactivation of the Bacterial Pathogens \u3cem\u3eStaphylococcus pseudintermedius\u3c/em\u3e and \u3cem\u3eAcinetobacter baumannii\u3c/em\u3e By Butanoic Acid

Aims This study was performed to evaluate the efficacy of butanoic acid against bacterial pathogens including Acinetobacter baumannii and Staphylococcus pseudintermedius. Methods and Results Vegetative bacteria were exposed to butanoic acid in vitro and log reduction was quantified using viable count assays. The maximum (8 and 9) log inactivation was determined by qualitatively assaying for growth/no-growth after a 48-h incubation (37°C). Membrane integrity after exposure to butanoic acid was determined by propidium iodide staining, scanning electron microscopy, membrane depolarization and inductively coupled plasma analysis. Cytosolic pH was measured by 5-(6-)carboxyfluorescein succinimidyl ester. Conclusions Inhibitory concentrations of butanoic acid ranged between 11 and 21 mmol l−1 for Gram-positive and Gram-negative species tested. The maximum log reduction of A. baumannii was achieved with a 10-s exposure of 0·50 mol l−1 of butanoic acid. Staphylococcus pseudintermedius required 0·40 mol l−1 of butanoic acid to achieve the same level of reduction in the same time period. Inactivation was associated with membrane permeability and acidification of the cytosol. Significance and Impact of the Study Antibiotic resistance among bacterial pathogens necessitates the utilization of novel therapeutics for disinfection and biological control. These results may facilitate the development of butanoic acid as an effective agent against a broad-spectrum of antibiotic-resistant bacterial pathogens

Thermomechanical Fatigue Behavior of a Silicon Carbide Fiber-Reinforced Calcium Aluminosilicate Composite

Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/65874/1/j.1151-2916.1993.tb04022.x.pd

Methylation levels of a novel genetic element, EgNB3 as a candidate biomarker associated with the embryogenic competency of oil palm

The association between DNA methylation status and embryogenic competency in oil palm tissue culture was examined through Representational Difference Analysis (RDA) approach, using methylation-sensitive restriction endonucleases. "Difference Products" (DPs) of RDA derived from palms of similar genetic backgrounds but exhibiting different embryogenesis rates during the regeneration process were isolated. The DPs were sequenced using a pyrosequencing platform. To our knowledge, this is the first study profiling partial HpaII methylation sites in oil palm young leaf tissues which are potentially associated with embryogenic amenability through a genomic subtractive approach. Quantitative real-time PCR analysis demonstrated that the methylation status of a novel fragment, EgNB3, was higher in highly embryogenic leaf explants compared to low embryogenesis rate materials. These differences are likely to be contributed by the 5′-mCCGG-3′ and/or 5′-mCmCGG-3′ methylation patterns. Our data suggest that the differentially methylated site in EgNB3 has potential as a molecular biomarker for the screening of oil palm leaf explants for their embryogenic potentials

Genotyping a second growth coast redwood forest : a high throughput methodology

The idea that excitonic (electronic) coherences are of fundamental importance to natural photosynthesis gained popularity when slowly dephasing quantum beats (QBs) were observed in the two-dimensional electronic spectra of the Fenna–Matthews–Olson (FMO) complex at 77 K. These were assigned to superpositions of excitonic states, a controversial interpretation, as the strong chromophore–environment interactions in the complex suggest fast dephasing. Although it has been pointed out that vibrational motion produces similar spectral signatures, a concrete assignment of these oscillatory signals to distinct physical processes is still lacking. Here we revisit the coherence dynamics of the FMO complex using polarization-controlled two-dimensional electronic spectroscopy, supported by theoretical modelling. We show that the long-lived QBs are exclusively vibrational in origin, whereas the dephasing of the electronic coherences is completed within 240 fs even at 77 K. We further find that specific vibrational coherences are produced via vibronically coupled excited states. The presence of such states suggests that vibronic coupling is relevant for photosynthetic energy transfer

Real-time observation of multiexcitonic states in ultrafast singlet fission using coherent 2D electronic spectroscopy.

Singlet fission is the spin-allowed conversion of a spin-singlet exciton into a pair of spin-triplet excitons residing on neighbouring molecules. To rationalize this phenomenon, a multiexcitonic spin-zero triplet-pair state has been hypothesized as an intermediate in singlet fission. However, the nature of the intermediate states and the underlying mechanism of ultrafast fission have not been elucidated experimentally. Here, we study a series of pentacene derivatives using ultrafast two-dimensional electronic spectroscopy and unravel the origin of the states involved in fission. Our data reveal the crucial role of vibrational degrees of freedom coupled to electronic excitations that facilitate the mixing of multiexcitonic states with singlet excitons. The resulting manifold of vibronic states drives sub-100 fs fission with unity efficiency. Our results provide a framework for understanding singlet fission and show how the formation of vibronic manifolds with a high density of states facilitates fast and efficient electronic processes in molecular systems.This is the author accepted manuscript. The final version is available from NPG via http://dx.doi.org/10.1038/nchem.237