27 research outputs found
Intracellular iron uptake is favored in Hfe-KO mouse primary chondrocytes mimicking an osteoarthritis-related phenotype
HFE-hemochromatosis is a disease characterized by a systemic iron overload phenotype mainly associated with mutations in the HFE protein (HFE) gene. Osteoarthritis (OA) has been reported as one of the most prevalent complications in HFE-hemochromatosis patients, but the mechanisms associated with its onset and progression remain incompletely understood. In this study, we have characterized the response to high iron concentrations of a primary culture of articular chondrocytes isolated from newborn Hfe-KO mice and compared the results with that of a similar experiment developed in cells from C57BL/6 wild-type (wt) mice. Our data provide evidence that both wt- and Hfe-KO-derived chondrocytes, when exposed to 50 mu M iron, develop characteristics of an OA-related phenotype, such as an increased expression of metalloproteases, a decreased extracellular matrix production, and a lower expression level of aggrecan. In addition, Hfe-KO cells also showed an increased expression of iron metabolism markers and MMP3, indicating an increased susceptibility to intracellular iron accumulation and higher levels of chondrocyte catabolism. Accordingly, upon treatment with 50 mu M iron, these chondrocytes were found to preferentially differentiate toward hypertrophy with increased expression of collagen I and transferrin and downregulation of SRY (sex-determining region Y)-box containing gene 9 (Sox9). In conclusion, high iron exposure can compromise chondrocyte metabolism, which, when simultaneously affected by an Hfe loss of function, appears to be more susceptible to the establishment of an OA-related phenotype.European Regional Development FundEuropean Union (EU) [EMBRC.PT Alg-01-0145-FEDER-022121, Norte-01-0145-FEDER-000012]Fundacao para a Ciencia e a TecnologiaPortuguese Foundation for Science and Technology [SFRH/BD/77056/2011]Portuguese Foundation for Science and TechnologyPortuguese Foundation for Science and TechnologyPortuguese Science and Technology FoundationPortuguese Foundation for Science and Technologyinfo:eu-repo/semantics/publishedVersio
Pattern recognition receptors as potential therapeutic targets in inflammatory rheumatic disease
The pattern recognition receptors of the innate immune system are part of the first line of defence against pathogens. However, they also have the ability to respond to danger signals that are frequently elevated during tissue damage and at sites of inflammation. Inadvertent activation of pattern recognition receptors has been proposed to contribute to the pathogenesis of many conditions including inflammatory rheumatic diseases. Prolonged inflammation most often results in pain and damage to tissues. In particular, the Toll-like receptors and nucleotide-binding oligomerisation domain-like receptors that form inflammasomes have been postulated as key contributors to the inflammation observed in rheumatoid arthritis, osteoarthritis, gout and systemic lupus erythematosus. As such, there is increasing interest in targeting these receptors for therapeutic treatment in the clinic. Here the role of pattern recognition receptors in the pathogenesis of these diseases is discussed, with an update on the development of interventions to modulate the activity of these potential therapeutic targets
Use and efficacy of bone morphogenetic proteins in fracture healing
Signal transduction in aging related disease
Fibronectin fragment-induced expression of matrix metalloproteinases is mediated by MyD88-dependent TLR-2 signaling pathway in human chondrocytes
RANDOMISED, PLACEBO-CONTROLLED TRIAL TO EVALUATE CLINICAL EFFICACY AND STRUCTURE MODIFYING PROPERTIES OF SUBCUTANEOUS ETANERCEPT IN PATIENTS WITH EROSIVE INFLAMMATORY HAND OSTEOARTHRITIS
RANDOMIZED, PLACEBO-CONTROLLED TRIAL TO EVALUATE CLINICAL EFFICACY AND STRUCTURE MODIFYING PROPERTIES OF SUBCUTANEOUS ETANERCEPT (ETN) IN PATIENTS WITH EROSIVE INFLAMMATORY HAND OSTEOARTHRITIS (OA)
Pathophysiology and treatment of rheumatic disease
Effect of Etanercept on Several Soluble Biomarkers in a Randomized Controlled Trial of Patients with Erosive Hand Osteoarthritis
Pathophysiology and treatment of rheumatic disease
RANDOMISED, PLACEBO-CONTROLLED TRIAL TO EVALUATE CLINICAL EFFICACY AND STRUCTURE MODIFYING PROPERTIES OF SUBCUTANEOUS ETANERCEPT IN PATIENTS WITH EROSIVE INFLAMMATORY HAND OSTEOARTHRITIS
Development and application of statistical models for medical scientific researc