Insertional mutagenesis strategies in zebrafish

We review here some recent developments in the field of insertional mutagenesis in zebrafish. We highlight the advantages and limitations of the rich body of retroviral methodologies, and we focus on the mechanisms and concepts of new transposon-based mutagenesis approaches under development, including prospects for conditional 'gene trapping' and 'gene breaking' approaches

Production of Organometallic Polymer-Based Biomaterials by Laser Two-Photon Polymerisation

Laser two-photon polymerisation is a 3D printing technique based on the process of two photon absorption, where a photosensitive molecule becomes a radical upon absorbing two photons with double the wavelength of its absorption peak. By moving the sample relative to the laser focal point, 3D structures with sub-micron features can be fabricated from a variety of materials. Here, we present a novel organometallic polymer biomaterial composed of aluminium isopropoxide, methacrylic acid and 3-methacryloxypropyltrihydroxysilane. We describe the preparation, fabrication, development aspects of this material and characterise some structures fabricated directly from computer-aided design (CAD) files using scanning electron microscopy (SEM). KEYWORDS: Biomaterials, Laser Two-Photo