Generic Schemes for Single Molecule Kinetics 2: Information Content of the Poisson Indicator

Recently, we described a pathway analysis technique (paper 1) for analyzing generic schemes for single-molecule kinetics based upon the first-passage time distribution. Here, we employ this method to derive expressions for the Poisson indicator, a measure of stochastic variation (essentially equivalent to the Fano factor and Mandel's Q parameter), for various renewal (memoryless) enzymatic reactions. We examine its dependence on substrate concentration, without assuming all steps follow Poissonian kinetics. Based upon fitting to the functional forms of the first two waiting time moments, we show that, to second order, the non-Poissonian kinetics are generally underdetermined but can be specified in certain scenarios. For an enzymatic reaction with an arbitrary intermediate topology, we identify a generic minimum of the Poisson indicator as a function of substrate concentration, which can be used to tune substrate concentration to the stochastic fluctuations and estimate the largest number of underlying consecutive links in a turnover cycle. We identify a local maximum of the Poisson indicator (with respect to substrate concentration) for a renewal process as a signature of competitive binding, either between a substrate and an inhibitor or between multiple substrates. Our analysis explores the rich connections between Poisson indicator measurements and microscopic kinetic mechanisms

Numerical investigation of high-speed droplet impact using a multiscale two-fluid approach

A single droplet impact onto solid surfaces remains a fundamental and challenging topic in both experimental and numerical studies with significant importance in a plethora of industrial applications, ranging from printing technologies to fuel injection in internal combustion engines. Under high-speed impact conditions, additional complexities arise as a result of the prompt droplet splashing and the subsequent violent fragmentation; thus, different flow regimes and a vast spectrum of sizes for the produced secondary flow structures coexist in the flow field. The present work introduces a numerical methodology to capture the multiscale processes involved with respect to local topological characteristics. The proposed methodology concerns a compressible Σ-Υ two-fluid model with dynamic interface sharpening based on an advanced flow topology detection algorithm. The model has been developed in OpenFOAM® and provides the flexibility of dealing with the multiscale character of droplet splashing, by switching between a sharp and a diffuse interface within the Eulerian-Eulerian framework in segregated and dispersed flow regions, respectively. An additional transport equation for the interface surface area density (Σ) introduces important information for the sub-grid scale phenomena, which is exploited in the dispersed flow regions to provide an insight into the extended cloud of secondary droplets after impact on the target. A high-speed water droplet impact case has been examined and evaluated against new experimental data; these refer to a millimetre size droplet impacting a solid dry smooth surface at velocity as high as 150m/s, which corresponds to a Weber number of ~7.6×10^5. At the investigated impact conditions compressibility effects dominate the early stages of droplet splashing. A strong shock wave forms and propagates inside the droplet, where transonic Mach numbers occur; local Mach numbers up to 2.5 are observed for the expelled surrounding gas outside the droplet. The proposed numerical approach is found to capture relatively accurately the phenomena and provide significant information regarding the produced flow structure dimensions, which is not available from the experiment

Linear response formula for piecewise expanding unimodal maps

The average R(t) of a smooth function with respect to the SRB measure of a smooth one-parameter family f_t of piecewise expanding interval maps is not always Lipschitz. We prove that if f_t is tangent to the topological class of f_0, then R(t) is differentiable at zero, and the derivative coincides with the resummation previously proposed by the first named author of the (a priori divergent) series given by Ruelle's conjecture.Comment: We added Theorem 7.1 which shows that the horizontality condition is necessary. The paper "Smooth deformations..." containing Thm 2.8 is now available on the arxiv; see also Corrigendum arXiv:1205.5468 (to appear Nonlinearity 2012

The rise of fully turbulent flow

Over a century of research into the origin of turbulence in wallbounded shear flows has resulted in a puzzling picture in which turbulence appears in a variety of different states competing with laminar background flow. At slightly higher speeds the situation changes distinctly and the entire flow is turbulent. Neither the origin of the different states encountered during transition, nor their front dynamics, let alone the transformation to full turbulence could be explained to date. Combining experiments, theory and computer simulations here we uncover the bifurcation scenario organising the route to fully turbulent pipe flow and explain the front dynamics of the different states encountered in the process. Key to resolving this problem is the interpretation of the flow as a bistable system with nonlinear propagation (advection) of turbulent fronts. These findings bridge the gap between our understanding of the onset of turbulence and fully turbulent flows.Comment: 31 pages, 9 figure

Business and Information Technology Alignment Measurement -- a recent Literature Review

Since technology has been involved in the business context, Business and Information Technology Alignment (BITA) has been one of the main concerns of IT and Business executives and directors due to its importance to overall company performance, especially today in the age of digital transformation. Several models and frameworks have been developed for BITA implementation and for measuring their level of success, each one with a different approach to this desired state. The BITA measurement is one of the main decision-making tools in the strategic domain of companies. In general, the classical-internal alignment is the most measured domain and the external environment evolution alignment is the least measured. This literature review aims to characterize and analyze current research on BITA measurement with a comprehensive view of the works published over the last 15 years to identify potential gaps and future areas of research in the field.Comment: 12 pages, Preprint version, BIS 2018 International Workshops, Berlin, Germany, July 18 to 20, 2018, Revised Paper

Harper operators, Fermi curves, and Picard-Fuchs equations

This paper is a continuation of the work on the spectral problem of Harper operator using algebraic geometry. We continue to discuss the local monodromy of algebraic Fermi curves based on Picard-Lefschetz formula. The density of states over approximating components of Fermi curves satisfies a Picard-Fuchs equation. By the property of Landen transformation, the density of states has a Lambert series as the quarter period. A $q$-expansion of the energy level can be derived from a mirror map as in the B-model.Comment: v2, 13 pages, minor changes have been mad

Alocação de recursos e rentabilidade das pesquisas originadas do Centro Nacional de Pesquisa de Soja.

Desenvolvimento da soja no Brasil; Beneficios gerados pela pesquisa no CNPSo; Propriedades da pesquisa e principais tecnologias geradas.bitstream/item/23241/1/Doc26.pd