Accounting for molecular stochasticity in systematic revisions: species limits and phylogeny of Paroaria

Different frameworks have been proposed for using molecular data in systematic revisions, but there is ongoing debate on their applicability, merits and shortcomings. In this paper we examine the fit between morphological and molecular data in the systematic revision of Paroaria, a group of conspicuous songbirds endemic to South America. We delimited species based on examination of > 600 specimens, and developed distance-gap, and distance- and character-based coalescent simulations to test species limits with molecular data. The morphological and molecular data collected were then analyzed using parsimony, maximum likelihood, and Bayesian phylogenetics. The simulations were better at evaluating the new species limits than using genetic distances. Species diversity within Paroaria had been underestimated by 60%, and the revised genus comprises eight species. Phylogenetic analyses consistently recovered a congruent topology for the most recently derived species in the genus, but the most basal divergences were not resolved with these data. The systematic and phylogenetic hypotheses developed here are relevant to both setting conservation priorities and understanding the biogeography of South America. 
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Dynamic allometry in coastal overwash morphology

Allometry refers to a physical principle in which geometric (and/or metabolic) characteristics of an object or organism are correlated to its size. Allometric scaling relationships typically manifest as power laws. In geomorphic contexts, scaling relationships are a quantitative signature of organization, structure, or regularity in a landscape, even if the mechanistic processes responsible for creating such a pattern are unclear. Despite the ubiquity and variety of scaling relationships in physical landscapes, the emergence and development of these relationships tend to be difficult to observe - either because the spatial and/or temporal scales over which they evolve are so great or because the conditions that drive them are so dangerous (e.g. an extreme hazard event). Here, we use a physical experiment to examine dynamic allometry in overwash morphology along a model coastal barrier. We document the emergence of a canonical scaling law for length versus area in overwash deposits (washover). Comparing the experimental features, formed during a single forcing event, to 5 decades of change in real washover morphology from the Ria Formosa barrier system, in southern Portugal, we find differences between patterns of morphometric change at the event scale versus longer timescales. Our results may help inform and test process-based coastal morphodynamic models, which typically use statistical distributions and scaling laws to underpin empirical or semi-empirical parameters at fundamental levels of model architecture. More broadly, this work dovetails with theory for landscape evolution more commonly associated with fluvial and alluvial terrain, offering new evidence from a coastal setting that a landscape may reflect characteristics associated with an equilibrium or steady-state condition even when features within that landscape do not.Funding Agency NERC Natural Environment Research Council NE/N015665/2 Leverhulme Trust RPG-2018-282info:eu-repo/semantics/publishedVersio

The Weinstein conjecture in the presence of submanifolds having a Legendrian foliation

Helmut Hofer introduced in '93 a novel technique based on holomorphic curves to prove the Weinstein conjecture. Among the cases where these methods apply are all contact 3--manifolds $(M,\xi)$ with $\pi_2(M) \ne 0$. We modify Hofer's argument to prove the Weinstein conjecture for some examples of higher dimensional contact manifolds. In particular, we are able to show that the connected sum with a real projective space always has a closed contractible Reeb orbit.Comment: 11 pages, 2 figure

Dual antibiotherapy of tuberculosis mediated by inhalable locust bean gum microparticles

Despite the existence of effective oral therapy, tuberculosis remains a deadly pathology, namely because of bacterial resistance and incompliance with treatments. Establishing alternative therapeutic approaches is urgently needed and inhalable therapy has a great potential in this regard. As pathogenic bacteria are hosted by alveolar macrophages, the co-localisation of antitubercular drugs and pathogens is thus potentiated by this strategy. This work proposes inhalable therapy of pulmonary tuberculosis mediated by a single locust bean gum (LBG) formulation of microparticles associating both isoniazid and rifabutin, complying with requisites of the World Health Organisation of combined therapy. Microparticles were produced by spray-drying, at LBG/INH/RFB mass ratio of 10/1/0.5. The aerodynamic characterisation of microparticles revealed emitted doses of more than 90% and fine particle fraction of 38%, thus indicating the adequacy of the system to reach the respiratory lung area, thus partially the alveolar region. Cytotoxicity results indicate moderate toxicity (cell viability around 60%), with a concentration-dependent effect. Additionally, rat alveolar macrophages evidenced preferential capture of LBG microparticles, possibly due to chemical composition comprising mannose and galactose units that are specifically recognised by macrophage surface receptors. (C) 2017 Elsevier B.V. All rights reserved.National Portuguese funding through FCT - Fundacao para a Ciencia e a Tecnologia [PTDC/DTP-FTO/0094/2012, UID/BIM/04773/2013, UID/Multi/04326/2013, UID/QUI/00100/2013, PEst-OE/QUI/UI4023/2011

FROM PAPER TO PLASTIC BY 2002: RETAILERS' PERSPECTIVE ON ELECTRONIC BENEFIT TRANSFER SYSTEMS FOR FOOD STAMPS

The Food Stamp Program (FSP) is working under the deadline of October 1, 2002, to coordinate a change from the current paper disbursement system of paper food stamps to an electronic transfer system of benefits, known as EBT. The Food and Nutrition Service of the U.S. Department of Agriculture has been studying the effectiveness of differing vehicles for benefit dispersal since the inception of the FSP in the 1960's. The merits of a direct cash payment have been compared to those of the paper system by the USDA and an array of professional groups and research organizations. The adoption of the electronic benefit transfer (EBT) system engenders a new set of questions about the effects of EBT on benefit recipients, retailers and the administering government agencies. Issues surrounding the transition from paper to plastic are still problematic for retailers in spite of the rapidly approaching deadline. National interoperability of EBT as well as fees involved with EBT operation are still points of contention for retailers. Anecdotal evidence from retailers also point to kinks in the institutional operation of EBT. Peak-loading problems with the electronic network system generate a host of undesirable consequences for benefit recipients and retailers. These problems impede the electronic system from taking advantage of the positive network effects that could arise from this new technology.Food Consumption/Nutrition/Food Safety, Food Security and Poverty, Marketing, Research and Development/Tech Change/Emerging Technologies,

Soy protein enzymatic hydrolysis and polysaccharides interactions: differential performance on kinetic adsorption at air-water interface

The objective of the work was to study the impact of soy protein hydrolysis on kinetic adsorption to the air-water interface and the effect of polysaccharides addition. Was used soy protein (SP) and theirs hydrolysates of 2% (H1) and 5.4% (H2) degree of hydrolysis. The polysaccharides (PS) used were a surface active one called E4M and a non-surface active one, lamda carrageenan (C). The dynamic surface pressure of interfacial films was evaluated with a drop tensiometer. In this contribution, we have determined the kinetic parameters of adsorption to the air-water interface which determined the penetration (Kp) and rearrangement (Kr) rates of SP, H1, H2 and PS, as well as their mixed systems. It was observed an increase of Kp and Kr when the protein were hydrolyzed (from SP to H1), however, when degree of hydrolysis progresses to H2 the parameters decreased again. In other hand, considerable differences were not found between these two PS studied concerning the Kp to air-water interface at these conditions. In spite of the different surface active nature of the PS, the proteins seem to control the behavior of the protein-PS interactions. However, when Kr of mixed systems was analyzed, the degree of hydrolysis and PS nature started to have a huge importance. Hence, it could be observed synergic or antagonic effects on Kr of biopolymers at liquid interface depending to the degree of hydrolysis of protein analyzed and the type of PS selected.CYTED through project 105PI0274CYCYT through grant AGL2007-60045Junta de Andalucía through grant PO6-AGR-01535Universidad de Buenos Aires, Agencia Nacional de Promoción Científica y Tecnológica (PICT 2008-1901) and Consejo Nacional de Investigaciones Científicas y Técnicas de la República Argentin

Loss of YABBY2-Like Gene Expression May Underlie the Evolution of the Laminar Style in Canna and Contribute to Floral Morphological Diversity in the Zingiberales.

The Zingiberales is an order of tropical monocots that exhibits diverse floral morphologies. The evolution of petaloid, laminar stamens, staminodes, and styles contributes to this diversity. The laminar style is a derived trait in the family Cannaceae and plays an important role in pollination as its surface is used for secondary pollen presentation. Previous work in the Zingiberales has implicated YABBY2-like genes, which function in promoting laminar outgrowth, in the evolution of stamen morphology. Here, we investigate the evolution and expression of Zingiberales YABBY2-like genes in order to understand the evolution of the laminar style in Canna. Phylogenetic analyses show that multiple duplication events have occurred in this gene lineage prior to the diversification of the Zingiberales. Reverse transcription-PCR in Canna, Costus, and Musa reveals differential expression across floral organs, taxa, and gene copies, and a role for YABBY2-like genes in the evolution of the laminar style is proposed. Selection tests indicate that almost all sites in conserved domains are under purifying selection, consistent with their functional relevance, and a motif unique to monocot YABBY2-like genes is identified. These results contribute to our understanding of the molecular mechanisms underlying the evolution of floral morphologies