The Application of the Essential Oils of Thymus vulgaris L. and Crithmum maritimum L. as Biocidal on Two Tholu Bommalu Indian Leather Puppets

The chemical profile of the Thymus vulgaris (Lamiaceae) essential oil (EO) was investigated in order to evaluate its biological properties against microorganisms affecting two Tholu Bommalu, typical Indian leather puppets stored at the International Puppets Museum “Antonio Pasqualino” of Palermo, Italy. A GC–MS analysis, using both polar and apolar columns, was used to determine the chemical composition of the essential oil. The aim of this study was to evaluate the antimicrobial effectiveness of the Thymus vulgaris and Crithmum maritimum essential oils in vapor phase to disinfect heritage leather puppets. Pieces of leather artifacts that were affected by different bacterial colonies were exposed to EO under vacuum and static evaporation conditions. The results presented showed that the vaporization of essential oil was an efficient method in the disinfection of natural skins, eradicating microorganism in short times. T. vulgaris EO in the 50% solution showed excellent inhibitory activity against isolated bacteria with both methods, but the obtained results suggest that the vacuum method allowed for faster exposition of the artifacts to the biocide. Furthermore, the biocidal properties of the essential oil of a Sicilian accession of Crithmum maritimum (Apiaceae) aerial parts were compared and investigated. The results of the latter essential oil showed a poor activity against the isolated micro-organism

Opuntia ficus-indica (L.) Mill. and Opuntia stricta (Haw.) Haw. Mucilage-Based Painting Binders for Conservation of Cultural Heritage

The possibility of using materials from the waste of agricultural products for the conserva- tion of cultural and artistic heritage has led to important technological developments on mortars, plasters, colors, and other applications. In this experimental work, we investigated the binding properties of mucilage obtained from two different species of the genus Opuntia, both collected in Sicily, Italy: Opuntia ficus-indica (L.) Mill. and Opuntia stricta (Haw.) Haw. Through chemical acid hydrolysis, and subsequent spectroscopic analysis conducted at 13C-NMR, the main monosaccharide composition of both mucilage was studied, identifying considerable compositional differences. In fact, the mucilage of O. ficus indica had similar total amounts of arabinose (23.65%), galactose (20.87%), and glucose isomers (23.89%), while that of O. stricta was characterized by significant amounts of arabinose (36.48%) and galactose (32.31%) units. The samples were obtained by dispersing pigments on the mucilage and applying the obtained tempera by a brush onto both paper and chalk supports, in order to observe if the colors changed with different substrates. Colorimetric analysis, measuring ∆E, showed how the same pigment modifies its aspect depending on the binder used. After a two-week UV ageing process, pigments that had dispersed in O. stricta changed their aspect more than those dispersed in O. ficus-indica. Overall, it is also evident how ∆E data for organic pigments are higher than those for inorganic ones

Identification of a BAZ2A Bromodomain Hit Compound by Fragment Joining

The bromodomains of BAZ2A and BAZ2B (bromodomain adjacent to zinc finger domain proteins 2) are among the most hard to drug of the 61 human bromodomains. While little is known about the role of BAZ2B, there is strong evidence for the opportunity of targeting BAZ2A in various cancers. Here, a benzimidazole–triazole fragment that binds to the BAZ2A acetyl lysine pocket was identified by a molecular docking campaign and validated by competitive binding assays and X-ray crystallography. Another ligand was observed in close proximity by soaking experiments using the BAZ2A bromodomain preincubated with the benzimidazole–triazole fragment. The crystal structure of BAZ2A with the two ligands was employed to design a few benzimidazole–triazole derivatives with increased affinity. We also present the engineering of a BAZ2A bromodomain mutant for consistent, high-resolution crystallographic studies

Off-label long acting injectable antipsychotics in real-world clinical practice: a cross-sectional analysis of prescriptive patterns from the STAR Network DEPOT study

Introduction Information on the off-label use of Long-Acting Injectable (LAI) antipsychotics in the real world is lacking. In this study, we aimed to identify the sociodemographic and clinical features of patients treated with on- vs off-label LAIs and predictors of off-label First- or Second-Generation Antipsychotic (FGA vs. SGA) LAI choice in everyday clinical practice. Method In a naturalistic national cohort of 449 patients who initiated LAI treatment in the STAR Network Depot Study, two groups were identified based on off- or on-label prescriptions. A multivariate logistic regression analysis was used to test several clinically relevant variables and identify those associated with the choice of FGA vs SGA prescription in the off-label group. Results SGA LAIs were more commonly prescribed in everyday practice, without significant differences in their on- and off-label use. Approximately 1 in 4 patients received an off-label prescription. In the off-label group, the most frequent diagnoses were bipolar disorder (67.5%) or any personality disorder (23.7%). FGA vs SGA LAI choice was significantly associated with BPRS thought disorder (OR = 1.22, CI95% 1.04 to 1.43, p = 0.015) and hostility/suspiciousness (OR = 0.83, CI95% 0.71 to 0.97, p = 0.017) dimensions. The likelihood of receiving an SGA LAI grew steadily with the increase of the BPRS thought disturbance score. Conversely, a preference towards prescribing an FGA was observed with higher scores at the BPRS hostility/suspiciousness subscale. Conclusion Our study is the first to identify predictors of FGA vs SGA choice in patients treated with off-label LAI antipsychotics. Demographic characteristics, i.e. age, sex, and substance/alcohol use co-morbidities did not appear to influence the choice towards FGAs or SGAs. Despite a lack of evidence, clinicians tend to favour FGA over SGA LAIs in bipolar or personality disorder patients with relevant hostility. Further research is needed to evaluate treatment adherence and clinical effectiveness of these prescriptive patterns

Scientific and interdisciplinary method as support for the restoration project. The balustrade steps of Villa Cerami

In this work an interdisciplinary study of the weathering forms of the Villa Cerami balustrade, was carried out with the aim to identify the type and causes of these and to plan conservation measures. The studied balustrade adorns and protects the steps of Villa Cerami garden, which is a suggestive example of 18th century ‘urban villa’, located in the very core of the Baroque Catania. Sadly, these stunning steps, whose magnificence and placement characterises the out-door environment of the building, at present suffer from bad degradation conditions, and the decorative details adorning the baluster are affected by irreversible damage. The causes of this ongoing degradation process are: material features, humidity, pollution and the consumption caused by the activities performed in the building. Since 1957 it has been the location of the Faculty of Law of the University of Catania. In this study, three balusters affected by the main weathering forms (biological colonization, black crust and granular disintegration) recognised in the entire balustrade, were selected. The lithological type and the weathering forms were defined on the basis of an in situ investigation, using respectively the comparison of materials, to identify the calcarenites type, and the Italian norm UNI 11182 along with the Fitzner formalism, to classify the degradation forms.  A 3D survey of the selected balusters was performed with a time of flight Laser Scanner HDS300 of the Leica Geosystem with the aim to better define the volume and total surfaces of the material parts affected by erosion. The surfaces affected by black crust, were obtained by means of an image modelling technique. Data were used to calculate the damage indices through equations proposed by Fitzner and the limit at break for crushing. The potentiality of this interdisciplinary approach (architects, engineers and geologists) is shown with the aim to apply it to the restoration of the entire monument. In addition, this interdisciplinary process has been useful to identify the strengths and weaknesses to assess the consequences of design choices with awareness

Exploring Interleukin Levels in Type 1 Diabetes and Periodontitis: A Review with a Focus on Childhood

Diabetes can trigger an increase in cytokine levels leading to the production of C-reactive protein and fibrinogen. These molecules promote subclinical inflammation, causing the expression of adhesive molecules and endothelial dysfunction. Despite the lack of a comprehensive panel for single-nucleotide polymorphisms (SNPs) for interleukins associated with type 1 diabetes mellitus (T1DM), understanding the inflammatory role of SNPs is crucial because periodontitis, the sixth complication of diabetes, is influenced via these genetic variations. This review focuses on the interleukin levels in T1DM patients with and without periodontitis, with a particular focus on childhood and on SNPs when reported. A search of PubMed and Scopus identified 21 relevant studies from the past five years. Several ILs were analyzed, emphasizing that T1DM still needs to be thoroughly explored regarding an IL polymorphisms panel; however, the last five years have led to the increased independence of this condition, causing autonomous inflammatory effects, which require further investigation. The periodontitis and T1DM association in children and adolescents represents a severe gap in the literature that should be filled; this scarce presence of studies serves as motivation for further clinical research

Patients-Reported Oral Manifestations in Coeliac Disease and Inflammatory Bowel Diseases: An Italian Survey

Inflammatory Bowel Diseases (IBDs), including Ulcerative Colitis (UC), Crohn’s Disease (CD), Irritable Bowel Syndrome (IBS), and Coeliac Disease (CoD), primarily affect the intestinal tract but show some extra-intestinal manifestations affecting the skin, joints, liver, pancreas, and lungs. The aim of this survey was to define how often these intestinal conditions show oral manifestation. The study involved 46 patients of 56 recruited at the beginning, with a mean age of 42 ± 13.64 and a sex distribution of 67.4% (31) female and 32.6% (15) male. Everyone was sent a questionnaire of four multiple-choice questions about the type of their bowel disease, the time of the diagnosis, the major symptoms which led to the disease discover, and finally their oral symptoms. According to the results, 65.52% referred to oral manifestations, especially canker sores and burning sensation. The oral involvement suggests that multidisciplinary management and therapy are mandatory, with close monitoring and follow-up interfacing with other specialists. Finally, dental practitioners have to be aware of this possible oral effect in IBDs and CoD patients

Diabetes and Non-Surgical Periodontal Therapy: What Can We Hope for?

Diabetes and periodontal disease share the same inflammatory pattern. Both these pathologies, if left untreated, lead to a cytokine storm that carries pro-inflammatory factors throughout the body. Periodontitis has recently been assumed to be the sixth complication of diabetes and the latest studies suggest a biunivocal connection between these two conditions. Most recent evidence-based studies propose that having a controlled periodontal situation with proper and timely therapy could improve glycemic management in diabetic patients. In order to provide the newest findings on this topic, a systematic literature research was performed on PubMed following the PRISMA statement. The keywords used were: “Diabetes; Periodontitis; Non-surgical periodontal therapy”. Only free full texts and abstracts in English were enrolled considering a time range of the last 10 years, from 2011 to 2021. A total of 308 studies arose from the first search, and only 73 were strictly related to our topic, while 235 were excluded. The bidirectional link between diabetes and periodontitis is well known. To the best of our knowledge, in the last 10 years there is increasing evidence that non-surgical periodontal treatment is associated with improved glycemic control. Further studies are needed to empower this relation