48 research outputs found
Aromatic Plants from Western Balkans: A Potential Source of Bioactive Natural Compounds
Documentation of traditionally used aromatic and medical plants has been carried out in many European countries over the last several years. Over the last decade, the Western Balkans has become the area of a huge number of ethnobiological field studies. Many of those focused on Balkans ethnobotany are linked to the long and ongoing history of gathering and trading local wild aromatic and medicinal plants from this territory into Western European markets. But only less than a half percent of these have been studied for their chemical composition and medicinal value. The most investigated aromatic species in this area belongs to the few biggest families: Asteraceae, Apiaceae, Lamiaceae and Rosaceae. Medicinal value of plants lies in some chemical substances that produce physiological action on the human body, which leads to positive effect on health. Essential oils are secondary metabolites which are the most examined, as well as various plant extracts. Isolation and identification of the compounds in combination with its biological screening can considerably contribute to plant studies. Also, application of new activities and novel techniques for susceptibility testing provide better knowledge of wild growing medicinal plants as potential sources of biological agents and justified their traditional uses
Radical scavenging and antimicrobial activity of essential oil and extracts of Echinophora sibthorpiana Guss. from Macedonia
This study was undertaken to determine the antioxidant and antimicrobial
effect of essential oil and extracts of Echinophora sibthorpiana Guss. (fam.
Apiaceae) collected in Macedonia. The chemical composition of E. sibthorpiana
essential oil was characterized by the presence of methyl eugenol (60.40%),
p-cymene (11.18%) and α-phellandrene (10.23%). The free radical scavenging
activity of extracts and essential oil was evaluated by DPPH and ABTS assays.
The aqueous extract of aerial parts exhibited the strongest scavenging
activity (IC50=1.67 mg/ml); results of the ABTS test showed that the most
effective was the ethanol extract of aerial parts (1.11 mg vit. C/g). The
essential oil showed stronger antioxidant activity compared to
hydroxyanisole, ascorbic acid and quercetin that were used in the DPPH and
ABTS tests, respectively. The total phenolic and flavonoid concentrations in
the extracts ranged between 38.65-60.72 mg GA/g, and 3.15-19.00 mg Qu/g,
respectively. The antimicrobial properties of the extracts and essential oil
were investigated using a micro-well dilution technique against human
pathogenic strains. The results were comparable with the effects of the
positive controls, streptomycin and fluconazole. These findings indicate that
E. sibthorpiana extracts and oil can be used in preventive treatments and as
an alternative for synthetic preservatives. [Projekat Ministarstva nauke
Republike Srbije, br. 173029 i br. 173021
Chemical composition and antifungal activity of Salvia sclarea (Lamiaceae) essential oil
Clary sage (Salvia sclarea L.) is native to Southern Europe and is cultivated worldwide. The essential oil of clary sage was analyzed as a potential antifungal agent. The main compounds in the oil were linalyl acetate (52.83%) and linalool (18.18%). Food poisoning agents, spoilage fungi, and plant and animal pathogens were among the tested fungal species. The microdilution method was used to establish minimal inhibitory concentrations (MIC) and minimal fungicidal concentrations (MFC). The commercial antimycotic bifonazole was used as a control. A concentration of 25 μl/ml showed fungicidal activity against Aspergillus, Penicillium, and Fusarium species and Trichoderma viride. For the species Mucor mucedo and Aspergillus viride, the MFC was 15μl/ml; for Candida albicans, it was 10 μl/ml, as in the case of bifonazole. Fungistatic and fungicidal activities of the oil against Cladosporium cladosporioides and Trichophyton menthagrophytes were recorded at concentrations of 2.5 μl/ml and 5 μl/ml. The most sensitive micromycetes were Cladosporium fulvum, Alternaria alternata, Phomopsis helianthi, and Phoma macdonaldii, where a concentration of 2.5 μl/ml was lethal.Šarlahna žalfija (Salvia sclarea L.) je kao samonikla vrsta rasprostranjena u Južnoj Evropi, a kultivisana širom sveta. U radu je analizirano etarsko ulje ove vrste i utvrđivana njegova antifungalna aktivnost. Glavne komponente etarskog ulja su linalil acetat (52.83%) i linalol (18.18%). Kao test organizmi korišćene su gljivice koje izazivaju kvarenje hrane, kao i patogeni biljaka i životinja. Korišćenjem mikrodilucione metode određivane su minimalne inhibitorne (MIC) i minimalne fungicidne koncentracije (MFC). Komercijalni antimikotik bifonazol je korišćen kao kontrola. U koncentraciji od 25 µl/ml ulje je delovalo fungicidno na vrste rodova Aspergillus, Penicillium i Fusarium i vrstu Trichoderma viride. Za vrste Mucor mucedo i Aspergillus viride MFC je bila 15 µl/ml, i 10 µl/ml za C. albicans, slično kao za bifonazol. Potpuno zaustavljanje rasta micelija Cladosporium cladosporioides i Trichophyton menthagrophytes je postignuto pri koncentracijama 2.5 µl/ml i 5 µl/ml. Najosetljivije mikromicete bile su Cladosporium fulvum, Alternaria alternata, Phomopsis helianthi i Phoma macdonaldii, za koje je letalna koncentracija bila 2.5 µl/ml.Projekat ministarstva br. 143041 i 14304
The chemical composition, antimicrobial and antioxidant activities of the essential oil of Salvia fruticosa growing wild in Libya
The composition of essential oil isolated from Salvia fruticosa, wild growing in Libya, was analyzed by GC and GC-MS. Forty-five compounds could be identified. The essential oil contained 1,8-cineole (49.34%), camphor (7.53%), β-pinene (7.38%), myrcene (7.38%), α-pinene (5.15%), β-caryophyllene (4.13%) and α-terpineol (3.25). Antioxidant activity was analyzed using the DPP H free radical scavenging method and low antioxidant activity was found (IC50 = 15.53 mg/ml). The oil was also screened for its antimicrobial activity against eight bacteria (four Gram-negative and four Gram-positive) and eight fungi. The essential oil of S. fruticosa showed minimal inhibitory activity (MIC) at 0.125-1.5 mg/ml and bactericidal (MBC) at 0.5-2.0 mg/ml. In addition, it exhibited fungistatic (MIC) at 0.125-1.0 mg/ml and fungicidal effect (MFC) at 0.125-1.5 mg/ml.Projekat ministarstva br. 173029 i br. 17303
Supplementary data for article: Mileski, K. S.; Trifunović, S. S.; Ćirić, A. D.; Šakić, Ž. M.; Ristić, M. S.; Todorović, N. M.; Matevski, V. S.; Marin, P. D.; Tešević, V. V.; Džamić, A. M. Research on Chemical Composition and Biological Properties Including Antiquorum Sensing Activity of Angelica Pancicii Vandas Aerial Parts and Roots. Journal of Agricultural and Food Chemistry 2017, 65 (50), 10933–10949. https://doi.org/10.1021/acs.jafc.7b04202
Supporting information for: [https://doi.org/10.1021/acs.jafc.7b04202]Related to published version: [http://cherry.chem.bg.ac.rs/handle/123456789/2572
Laserpitium ochridanum: antioxidant, antimicrobial and anti-quorum sensing activities against Pseudomonas aeruginosa
This study shows Laserpitium ochridanum essential oil composition, its antifungal potency, and possible antioxidant, antimicrobial and anti-quorum sensing activities of different extracts. Monoterpene hydrocarbons (40.9%) found to be the most abundant group of constituents in the oil. Sabinene (22.8%), viridiflorol (14.7%) and α-pinene (11.40%) were the main compounds of the oil. Ethanolic extract had the highest antioxidant capacity in DPPH and ABTS assays and was the richest in polyphenols. Microdilution method revealed the strongest antibacterial activity of ethanolic extracts in comparison to other tested extracts and streptomycin. L. ochridanum oil evidenced the best antifungal potential against used micromycetes. Results of anti-quorum sensing activity assay indicated high affection of aqueous extract in reduction of PAO1 pyocianin production (18.07%).Used samples possessed slight reduction of twitching and swimming mobility. Excellent antifungal oil activity, good antioxidant, antimicrobial and anti-quorum activity of L. ochridanum extracts was found. This study shows for the first time anti-guorum sensing activity against Pseudomonas aeruginosa of L. ochridanum, as well as its significant antioxidant potential. This study was conducted due to adventives of usage of natural products as efficient substitution for synthetic agents in treatment of various medical conditions
Chemical composition and antifungal activity of Salvia sclarea (Lamiaceae) essential oil
Clary sage (Salvia sclarea L.) is native to Southern Europe and is cultivated worldwide. The essential oil of clary sage was analyzed as a potential antifungal agent. The main compounds in the oil were linalyl acetate (52.83%) and linalool (18.18%). Food poisoning agents, spoilage fungi, and plant and animal pathogens were among the tested fungal species. The microdilution method was used to establish minimal inhibitory concentrations (MIC) and minimal fungicidal concentrations (MFC). The commercial antimycotic bifonazole was used as a control. A concentration of 25 μl/ml showed fungicidal activity against Aspergillus, Penicillium, and Fusarium species and Trichoderma viride. For the species Mucor mucedo and Aspergillus viride, the MFC was 15μl/ml; for Candida albicans, it was 10 μl/ml, as in the case of bifonazole. Fungistatic and fungicidal activities of the oil against Cladosporium cladosporioides and Trichophyton menthagrophytes were recorded at concentrations of 2.5 μl/ml and 5 μl/ml. The most sensitive micromycetes were Cladosporium fulvum, Alternaria alternata, Phomopsis helianthi, and Phoma macdonaldii, where a concentration of 2.5 μl/ml was lethal.Šarlahna žalfija (Salvia sclarea L.) je kao samonikla vrsta rasprostranjena u Južnoj Evropi, a kultivisana širom sveta. U radu je analizirano etarsko ulje ove vrste i utvrđivana njegova antifungalna aktivnost. Glavne komponente etarskog ulja su linalil acetat (52.83%) i linalol (18.18%). Kao test organizmi korišćene su gljivice koje izazivaju kvarenje hrane, kao i patogeni biljaka i životinja. Korišćenjem mikrodilucione metode određivane su minimalne inhibitorne (MIC) i minimalne fungicidne koncentracije (MFC). Komercijalni antimikotik bifonazol je korišćen kao kontrola. U koncentraciji od 25 µl/ml ulje je delovalo fungicidno na vrste rodova Aspergillus, Penicillium i Fusarium i vrstu Trichoderma viride. Za vrste Mucor mucedo i Aspergillus viride MFC je bila 15 µl/ml, i 10 µl/ml za C. albicans, slično kao za bifonazol. Potpuno zaustavljanje rasta micelija Cladosporium cladosporioides i Trichophyton menthagrophytes je postignuto pri koncentracijama 2.5 µl/ml i 5 µl/ml. Najosetljivije mikromicete bile su Cladosporium fulvum, Alternaria alternata, Phomopsis helianthi i Phoma macdonaldii, za koje je letalna koncentracija bila 2.5 µl/ml.Projekat ministarstva br. 143041 i 14304
The chemical composition, antimicrobial and antioxidant activities of the essential oil of Salvia fruticosa growing wild in Libya
The composition of essential oil isolated from Salvia fruticosa, wild growing in Libya, was analyzed by GC and GC-MS. Forty-five compounds could be identified. The essential oil contained 1,8-cineole (49.34%), camphor (7.53%), β-pinene (7.38%), myrcene (7.38%), α-pinene (5.15%), β-caryophyllene (4.13%) and α-terpineol (3.25). Antioxidant activity was analyzed using the DPP H free radical scavenging method and low antioxidant activity was found (IC50 = 15.53 mg/ml). The oil was also screened for its antimicrobial activity against eight bacteria (four Gram-negative and four Gram-positive) and eight fungi. The essential oil of S. fruticosa showed minimal inhibitory activity (MIC) at 0.125-1.5 mg/ml and bactericidal (MBC) at 0.5-2.0 mg/ml. In addition, it exhibited fungistatic (MIC) at 0.125-1.0 mg/ml and fungicidal effect (MFC) at 0.125-1.5 mg/ml.Projekat ministarstva br. 173029 i br. 17303
The effect of antioxidant status on overall survival in renal cell carcinoma
Introduction The oxidative stress contributes to all three phases of carcinogenesis and represents a concomitant condition in renal cell carcinoma (RCC). RCC is the most common type of neoplasm of the kidney, and despite numerous studies the set of predictive and prognostic markers of survival are still unknown. The aim of our study was to examine the relation between antioxidant (AO) status and overall survival (OS) in RCC patients. Material and methods Our study included 95 patients with RCC, who underwent radical nephrectomy. We analysed the prognostic role of antioxidant enzymes (superoxide dismutase, catalase, glutathione peroxidase, glutathione S-transferase, glutathione reductase, glutathione, and malondialde-hyde) and other clinicopathological factors (size, grade, stage, and histological subtype) on the OS of RCC patients. Results The 5-year OS was 54.6%. The survival analysis related to AO parameters showed no significant difference in survival of RCC patients. The concentration of malondialdehyde, an indicator of lipid peroxidation, also had no significant effect on the survival rate of RCC pa-tients. Univariate and multivariate analysis confirmed the significance of clinicopathological parameters (size, p < 0.001; Fuhrman grade, p = 0.001, and stage, p < 0.001) for patients’ survival. Conclusions In our cohort of patients, different antioxidant parameters were not found to be predictors for OS of patients with RCC, who underwent radical nephrectomy
The effect of antioxidant status on overall survival in renal cell carcinoma
Introduction The oxidative stress contributes to all three phases of carcinogenesis and represents a concomitant condition in renal cell carcinoma (RCC). RCC is the most common type of neoplasm of the kidney, and despite numerous studies the set of predictive and prognostic markers of survival are still unknown. The aim of our study was to examine the relation between antioxidant (AO) status and overall survival (OS) in RCC patients. Material and methods Our study included 95 patients with RCC, who underwent radical nephrectomy. We analysed the prognostic role of antioxidant enzymes (superoxide dismutase, catalase, glutathione peroxidase, glutathione S-transferase, glutathione reductase, glutathione, and malondialde-hyde) and other clinicopathological factors (size, grade, stage, and histological subtype) on the OS of RCC patients. Results The 5-year OS was 54.6%. The survival analysis related to AO parameters showed no significant difference in survival of RCC patients. The concentration of malondialdehyde, an indicator of lipid peroxidation, also had no significant effect on the survival rate of RCC pa-tients. Univariate and multivariate analysis confirmed the significance of clinicopathological parameters (size, p < 0.001; Fuhrman grade, p = 0.001, and stage, p < 0.001) for patients’ survival. Conclusions In our cohort of patients, different antioxidant parameters were not found to be predictors for OS of patients with RCC, who underwent radical nephrectomy