4 research outputs found
Anti-atherogenic properties associated with the antioxidant activity from the hydrophilic extracts of Halimeda incrassata (Chlorophyta, Bryopsidales)
Seaweeds are a source of natural antioxidants having potential application in oxidative stress and associated diseases. In this work, anti-atherogenic properties associated with the antioxidant activity from the hydrophilic extracts of Halimeda incrassata were studied. The phenolic content assessed inthe aqueous extract and fraction phenolic acids (FPA) was 0.13 卤 0.05 and 0.47 卤 0.09 mg of gallic acid equivalents (GAE)/g dry seaweed, respectively. In DPPH?, radical scavenging assay fractions exhibited a dependent concentration. The seaweeds extract inhibited the desoxirribose oxidation in the presenceor absence of EDTA (IC50 = 1.91卤 0.09 mg/mL) (IC50 = 2.95 卤 0.01 mg/mL). In vivo antioxidant properties of FPA-H.incrassata were investigated in rats with a CCl4-induced liver injury. Pre-treatment with H.incrassata led to approximately 50% reductions in liver TBARS levels. The treatment with H. incrassataFPA also increased the activity of the CAT enzyme, which in turn resulted in an enhanced antioxidantdefense. The expression of Catalase by PCR-RT technique demonstrated a higher gene expression when compared with that which was observed in the CCl 4-treated group. Antiatherogenic properties were studied in the inhibition of lipoprotein oxidation mediated by Cu2+ or HRP/H2O2, free radicalscavenging, and metal ion chelation, and it was dose dependent with a higher concentration needed for the aqueous extract than for the FPA fraction. Antioxidant activity was also improved in macrophages as evaluated in the cell supernatant (by TBARS formation); and by luminol enhanced chemiluminescence after cell activation with zymosan; and a degree of cell lipoperoxidation wasdecreased by the Halimeda incrassata extract. The results of this work add to the antioxidant potential of the seaweed for its application in oxidative stress associated conditions.Fil: Vidal-Novoa, Alexis. Universidad de la Habana. Facultad de Biolog铆a; CubaFil: Costa-Mugica, Ariadna. Universidad de la Habana. Facultad de Biolog铆a; CubaFil: Zulueta D铆az, Yenisleidy de Las Mercedes. Universidad de la Habana. Facultad de Biolog铆a; Cuba. Consejo Nacional de Investigaciones Cient铆ficas y T茅cnicas. Centro Cient铆fico Tecnol贸gico Conicet - C贸rdoba. Centro de Investigaciones en Qu铆mica Biol贸gica de C贸rdoba. Universidad Nacional de C贸rdoba. Facultad de Ciencias Qu铆micas. Centro de Investigaciones en Qu铆mica Biol贸gica de C贸rdoba; ArgentinaFil: Diaz-Gutierrez, Dayl铆n. Universidad de la Habana. Facultad de Biolog铆a; CubaFil: de Oliveira e Silva, Ana, Mara. Universidade de Sao Paulo; BrasilFil: Vazquez, Ana Mar铆a. Center For Molecular Inmunology; CubaFil: Claudina, Zald铆var-Munoz. Universidad de la Habana. Facultad de Biolog铆a; CubaFil: Dalva, Assuncao Portari de Mancini. Institute Butantan Sao Paulo; BrasilFil: Mancini-Filho, Jorge. Universidade de Sao Paulo; Brasi
Comparation of antioxidants properties and polyphenols content of aqueous extract from seaweeds Bryothamnion triquetrum and Halimeda opuntia
Objetivos. Evaluar y comparar las propiedades antioxidantes mediante ensayos in vitro de extractos
acuosos de las algas roja Bryothamnion triquetrum y verde Halimeda opuntia y su relaci贸n con el contenido
de polifenoles.
Material y M茅todos. Se utilizaron las t茅cnicas in vitro: DPPH, Capacidad reductora, Inhibici贸n de la
peroxidaci贸n lip铆dica e inhibici贸n de la hem贸lisis inducida por AAPH.
Resultados. B. triquetrum: DPPH; CI50=1,15 卤 0,06, capacidad reductora a concentraci贸n; 128 mg/mL,
DO=2,798, inhibici贸n de la peroxidaci贸n lip铆dica; CI50=5,09卤 0,25 e inhibici贸n de la hem贸lisis con 12 mg/
mL; 35 %. H. opuntia: DPPH; CI50=12,34 卤 0,30 mg/mL, capacidad reductora; DO=0,800, inhibici贸n de la
peroxidaci贸n lip铆dica; CI50=1,25卤 0,31 mg/mL e inhibici贸n de la hem贸lisis; 82%.
Conclusiones. Los resultados evidencian que B triquetrum resulto mucho m谩s eficiente en los ensayos de
DPPH y Capacidad reductora mientras H opuntia resulta m谩s eficiente en Inhibici贸n de la peroxidaci贸n
lip铆dica e inhibici贸n de la hem贸lisis. Se discuten algunos aspectos acerca de sus posibles mecanismos de
acci贸n.Objective. To evaluate and compare the antioxidant activity displayed by seaweed H. opuntia and B.
triquetrum using different experimental in vitro antioxidant assessment models.
Material and Methods. The following techniques are utilized: DPPH, Reducing power, inhibition of
lipid peroxidation and inhibition of haemolysis.
Results. B. triquetrum: DPPH; IC50=1.15 卤 0.06, Reducing power (concentration 128 mg/mL), OD=2.798,
inhibition of lipid peroxidation; IC50=5.09卤 0.25 and inhibition of haemolysis; with 12 mg/mL; 35 %. H.
opuntia: DPPH; IC50=12.34 卤 0.30 mg/mL, reducing power; OD=0.800, inhibition of lipid peroxidation;
IC50=1.25卤 0.31 mg/mL, inhibition of lipid peroxidation; IC50=1.25卤 0.31 mg/mL and inhibition of haemolysis;
82%.
Conclusion. It was demonstrated that B.triquetrum extract was more effective than H. opuntia in reducing
power and DPPH assays while H.opuntia was more effective in inhibition of lipid peroxidation in rat
brain homogenates and the inhibition of red blood cell (RBC) haemolysis induced by AAPH. We discuss
some aspects about their possible mechanisms of action.Este trabajo fue financiado parcialmente
por CNPq (Brasil) a trav茅s del Proyecto
No. 401852/2012-1
El capital humano como principal impulsor del desarrollo local en la innovaci贸n tecnol贸gica en la agricultura en Cuba
This article seeks to show the principles of technological innovation, in addition to highlighting the importance of the development of human capital to increase technological innovation in agriculture, in order to enhance local development, its benefits for society, the economy and man a social being within future generations. This will contribute significantly to achieving growing and sustainable results, in addition to improving food security and the quality of life of the inhabitants.Este art铆culo busca mostrar los principios de la innovaci贸n tecnol贸gica, adem谩s de evidenciar la importancia que tiene el desarrollo del capital humano para aumentar la innovaci贸n tecnol贸gica en la agricultura, con el fin de elevar el desarrollo local, sus beneficios para la sociedad, la econom铆a y el hombre como ser social dentro de las futuras generaciones. Ello contribuir谩 de forma importante a lograr resultados crecientes y sostenibles, adem谩s del mejoramiento de la seguridad alimentaria y la calidad de vida de los habitantes
Comparaci贸n de las propiedades antioxidantes y contenido de polifenoles de extractos acuosos de las algas marinas Bryothamnion triquetrum y Halimeda opuntia
Objetivos: Evaluar y comparar las propiedades antioxidantes mediante ensayos in vitro de extractos acuosos de las algas roja Bryothamnion triquetrum y verde Halimeda opuntia y su relaci贸n con el contenido de polifenoles. Material y M茅todos: Se utilizaron las t茅cnicas in vitro: DPPH, Capacidad reductora, Inhibici贸n de la peroxidaci贸n lip铆dica e inhibici贸n de la hem贸lisis inducida por AAPH. Resultados: B. triquetrum: DPPH; CI50=1,15 卤 0,06, capacidad reductora a concentraci贸n; 128 mg/mL, DO=2,798, inhibici贸n de la peroxidaci贸n lip铆dica; CI50=5,09卤 0,25 e inhibici贸n de la hem贸lisis con 12 mg/mL; 35 %. H. opuntia: DPPH; CI50=12,34 卤 0,30 mg/mL, capacidad reductora; DO=0,800, inhibici贸n de la peroxidaci贸n lip铆dica; CI50=1,25卤 0,31 mg/mL e inhibici贸n de la hem贸lisis; 82%. Conclusiones: Los resultados evidencian que B triquetrum resulto mucho m谩s eficiente en los ensayos de DPPH y Capacidad reductora mientras H opuntia resulta m谩s eficiente en Inhibici贸n de la peroxidaci贸n lip铆dica e inhibici贸n de la hem贸lisis. Se discuten algunos aspectos acerca de sus posibles mecanismos de acci贸n