Physiological responses to water stress and stress memory in Argania spinosa

Drought stress is one of the major abiotic stress factors shifting the physiology and metabolism of the plants. In semiarid areas, drought stress events are often recurrent, and plants have developed strategies to remember a first so-called priming stress to eventually respond more effectively to a second triggering stress. In this study, we tested several physiological and morphological variables in Argania spinosa (L.) Skeels plants, growing in greenhouse conditions under a drought treatment induced by water withholding. Two sequential one-month periods of water stress were imposed to understand the stress memory events in this species The plants were divided into two groups depending on the treatment applied (irrigated and stressed) which later, after one month of a recuperation phase, were divided again into two more groups (having a total of four at the end of the study). Leaf samples were periodically taken and relative water content, gas exchange, chlorophyll content and other variables were measured, analysed and compared between those groups. Plants, which have suffered the two sequential water stress periods, were more affected according to several variables than plants subjected to only one stress treatment, so we finally conclude that this species does not seem to have any drought stress memory mechanisms for the studied variables, under the conditions of this experiment

The hemiparasitic shrub Osyris lanceolata (Santalaceae) does not disturb the ecophysiology of its hosts

Osyris lanceolata is a hemiparasitic dioecious shrub species growing on stabilised dunes. This study aimed to determine the spatial structure of O. lanceolata and its hosts, to understand the ecophysiological mechanisms involved in water and nutrient transfer, and quantify the physiological stress on its hosts. In the Asperillo stabilised dunes (Doñana, Spain) we selected one mixed plot (50 m × 50 m) with Pinus pinea and Juniperus phoenicea in which the coordinates of every individual, the hosts and the hemiparasitic species, were recorded. Additionally, we selected two study areas in which O. lanceolata was well represented, one dominated by P. pinea and the other by J. phoenicea. We marked 60 plants: 20 O. lanceolata with their respective hosts (10 Pinus and 10 Juniperus) and 20 free host plants. In all the marked plants we measured: leaf water potential, gas exchange, photochemical efficiency, morpho-anatomical leaf traits, pigments, and proline content. Our results evidenced that O. lanceolata is spatially associated with its hosts. Midday leaf water potential values were always between 1.5 to 3 times more negative and transpiration rates were 6-fold higher for the hemiparasite than for the hosts. Additionally, O. lanceolata plants showed a high accumulation of proline in leaves and haustorium. Although the hemiparasitic species maintains an active photosynthetic canopy, its rates of CO2 assimilation were 35–48% lower than in the hosts, which caused a 10-fold lower instantaneous water use efficiency. Through these mechanisms, the hemiparasitic plant can absorb water and nutrients from the host species. Despite this parasitic relationship, there were no significant differences in the physiological performance of the hosts in comparison to the parasite-free plants. We conclude that O. lanceolata presents a specific strategy to absorb water in this dry ecosystem, without exerting negative effects on the plant community, which might indicate that there is an equilibrium in the tradeoffs between parasitism and mutualism in the interaction between species

Reliance on deep soil water in the tree species Argania spinosa

In South-western Morocco, water scarcity and high temperature are the main factors determining species survival. Argania spinosa (L.) Skeels is a tree species, endemic to Morocco, which is suffering from ongoing habitat shrinkage. Argan trees play essential local ecological and economic roles: protecting soils from erosion, shading different types of crops, helping maintain soil fertility and, even more importantly, its seeds are used by the local population for oil production, with valuable nutritional, medicinal and cosmetic purposes. The main objective of this study was to identify the sources of water used by this species and to assess the effect of water availability on the photosynthetic rate and stem water potential in two populations: one growing on the coast and a second one 10 km inland. Stem water potential, photosynthetic rate and xylem water isotopic composition (δ18O) were seasonally monitored during 2 years. Trees from both populations showed a similar strategy in the use of the available water sources, which was strongly dependent on deep soil water throughout the year. Nevertheless, during the wet season or under low precipitation a more complex water uptake pattern was found with a mixture of water sources, including precipitation and soil at different depths. No evidence was found of the use of either groundwater or atmospheric water in this species. Despite the similar water-use strategy, the results indicate that Argania trees from the inland population explored deeper layers than coastal ones as suggested by more depleted δ18O values recorded in the inland trees and better photosynthetic performance, hence suggesting that the coastal population of A. spinosa could be subjected to higher stress

Fluctuaciones de la estructura vertical del matorral mediterráneo

En la naturaleza se pueden separar dos tipos de estructuras: Cristalinas y disipativas. Las primeras se mantienen indefinidamente (en ausencia de perturbación), las segundas, contrariamente, requieren para mantenerse un flujo continuo de energía (y a veces de materia) siendo sede de procesos disipativos permanentes (PRIGOGINE, 1983). Las estructuras disipativas, parecen contradecir el segundo principio de la termodinámica que predice que todos los sistemas evolucionan unidireccionalmente hacia un aumento irreversible de entropía, por su tendencia a incrementarse en complejidad a lo largo del tiempo. Esta característica de aumento de complejidad en las estructuras alcanza su máxima expresión en los sistemas biológicos. En ellos las estructuras disipativas existen en una jerarquía compleja, encontrándose desde los niveles de organización inferiores en las membranas y orgánulos celulares, hasta los superiores, en el ecosistema.

Germination and Clonal Propagation of the Endemic Shrub Corema album, a Vulnerable Species with Conservation Needs and Commercial Interest

In this study, we aimed to explore regeneration possibilities of Corema album (L.) D. Don by determining germination mechanisms and testing vegetative propagation methods. We analyzed seed viability under natural conditions, carried out germination treatments and a greenhouse experiment to study clonal propagation. We confirmed that C. album seeds present physiological dormancy, broken by ingestion by natural dispersers (rabbits and foxes), and that seed viability under natural conditions is lost after one year. In vitro germination was better achieved with a 200 ppm gibberellic acid treatment. Clonal propagation proved to be a successful technique for the production of C. album. Treating cuttings with IBA 0.2, w/v, at 20% resulted in the highest rooting percentage, while planting rooted cuttings in a substrate of perlite with vermiculite 1:1 was essential for plant survival. Our results show that both germination pretreatments and cutting propagation are powerful tools for the production of this valuable species. Both methods could be incorporated for population regeneration in natural habitats, and for the potential establishment of the species as a new crop for consumption and pharmacological purposes

Climatic conditions and herbivory effects on morphological plasticity of Argania spinosa

The main objective of this paper was to look into the morphological differentiation patterns and phenotypic plasticity in four populations of Argania spinosa with environmentally contrasted conditions. Mean response, magnitude and pattern of morphological intra- and inter-population plasticity indexes were measured and analyzed in order to identify which characters contribute the most to the acclimation of this species. Populations growing in the ecological optimum of the species presented the lowest plasticity, while those growing in the most stressed habitats showed an increased morphological variability. The study of four populations showed that human pressure seems to play an important function in the regulation of morphological characters. However, climatic conditions seem to play a significant role in the increase of morphological plasticity

Some secrets of Argania spinosa water economy in a semiarid climate

Argania spinosa is an example of an avoider tree growing under semi-arid conditions in Morocco. To assess what are the physiological strategies of this species, different variables were measured through an annual cycle in two populations located in the species’ main distribution area. Results show the expected decrease of leaf water potential (Ψ) with an increase of water-use efficiency (A/gs) with the onset of the dry season. In summer, leaf conductance (gs) was sensitive to vapour pressure deficit (VPD), and stomatal closure occurred over 30 mbar of VPD. Surprisingly, carbon isotope discrimination (δ13C) maintained very low values over the year, with almost no relationship with any physiological or morphological variable. Hence Argania spinosa presents a complex set of mechanisms to avoid water deficit, but δ13C cannot be used as an ecological tracer of long term WUE

Data from: Contrasting plant water-use responses to groundwater depth in coastal dune ecosystems

1.Groundwater lowering can produce dramatic changes in the physiological performance and survival of plant species. The impact of decreasing water availability due to climate change and anthropogenic groundwater extraction on coastal dune ecosystems has become of increasing concern, with uncertainties about how vegetation will respond in both the short and long terms. 2.We aimed to evaluate the water‐use responses of different plant functional types to increasing groundwater table depth and how this would affect their physiology in Mediterranean coastal dune systems differing in aridity. 3.We modeled water table depth, quantified the contribution of different soil layers to plant water through Bayesian isotope mixing models, and used a combination of spectral and isotope data to characterize plant ecophysiology. We found that increasing depth to groundwater triggered water uptake adjustments towards deeper soil layers only in the dry season. These adjustments in water sources use were made by conifer trees (Pinus pinea, P. pinaster) and hygrophytic shrubs (Erica scoparia, Salix repens) but not by the xerophytic shrub Corema album. Moreover, we observed a greater use of groundwater under semi‐arid conditions. Accompanying the greater use of water from deep soil layers as a response to increasing groundwater depth, the semi‐arid dimorphic‐rooted conifer tree P. pinea and hygrophytic shrub E. scoparia declined their water content (WI), without implications on photosynthetic parameters, such as chlorophyll content (CHL), photochemical index (PRI) and δ13C. Unexpectedly, under semi‐arid conditions, the shallow‐rooted xerophytic shrub C. album, associated with an absence of water‐sources‐use adjustments, showed a decline in WI, CHL, and PRI with groundwater table lowering. 4.We provide insight into how different species, belonging to different functional types, are acclimating to groundwater changes in a region experiencing climatic drought and a scarcity in groundwater due to anthropogenic exploitation. Greater depth to groundwater combined with limited precipitation can have a significant effect on plants’ water‐sources use and ecophysiology in semi‐arid coastal dune ecosystems

Isotopic data from semiaridMed site

Isotopic data (leaf d13C, xylem d18O) form semi-aridMed site at both spring and summer sampling date

Reflectance data from semi-aridMed site

Summer (dry season) reflectance index data (WI, CHL, PRI) from semi-aridMed sit