A study to evaluate the preservative action of Twak Arka in Triphala Kwatha

Introduction: Primary dosage forms are basic preparations whose shelf life is a challenge for the practice. Kwatha is a preparation which is easily prone to contamination and can be marketed only by the addition of suitable preservatives to increase the shelf life. So addition of preservatives is being practiced to prolong the shelf life of kwatha but presently using chemical preservatives are harmful to body and even have carcinogenic effects. Hence there aroused a need to find natural preservatives. Arka which is a water distillate consists of essential substances from the crude drug and has longer shelf life. Twak arka possess anti- microbial and anti- oxidant properties and economically cheaper and easily available drug and triphala kwatha being useful in many purposes. In this study an attempt was made to elucidate the preservative action of twak arka in triphala kwatha. Materials and methods: Includes preparation of twak arka, triphala kwatha and conduction of analytical and microbiological study to see the preservative action using SDA and MHA media. Observations and results: Study follows observations over microbial growth of the sample on daily basis where twak arka showed preservative action for 31 days. Aspergillus niger was the fungal growth seen on 32nd day. Discussion: Twak arka owing to its pH, chemical constituents and other properties preserved the triphala kwatha for a stipulated period of time. Conclusion: From the study, it was concluded that the twak arka preserved triphala kwatha without any microbial contamination for 31 days which was added in the concentration of 15%

Modification of Kakubhadi Churna into malahara and its physicochemical analysis

Samskara is the process of modification of Guna of a given substance which is responsible for conversion of a raw material into medicine. These Samskara are responsible for the change in quality and property of any medicine. Primary preparations of Bhaisajya Kalpana known as Panchavidha kashaya Kalpana are modified into secondary dosage forms like Vati, Avaleha, Malahara, Sneha kalpana etc in order to achieve better shelf life, easy administration and palatability. Churna kalpana is an Upkalapana of Kalka kalpana, it is mentioned to be used in many disease conditions. One among these is Kakubhadi churna, which is indicated to be sprinkled externally on Dushta Vrana. As this method of application is cumbersome and not patient friendly, an attempt has been made to modify Kakubhadi churna into Malahara. Malahara is similar to ointments and creams in modern pharmaceutics. When compared to Churna, it has as an extended shelf life and also drugs in the form of Malahara are easy to apply and store. Taking all this under consideration, present study was taken up to modify Kakubhadi churna into Malahara. Physico chemical analysis of prepared Churna and Malahara was also done to achieve preliminary standard

Preliminary analytical study of Ashvagandhadyarishta prepared from madhu and guda as sweetening agents

Introduction: Asava and arishta are medicinal preparations mentioned in Ayurvedic texts during which it undergoes a process of fermentation generating alcohol thus facilitating the extraction of the active principles contained in the drugs. Ashvagandhadyarishta is a type of arishta preparation where madhu (honey) is mentioned as madhura dravya (sweetening agent) in the formulation. So in this study an attempt is made to prepare Ashvagandhadyarishta in two batches by adding madhu as madhura dravya in one batch and guda (jaggery) as madhura dravya in another batch and to compare their organoleptic and analytical parameters. Materials and Methods: Study includes two batches of Ashvagandhadyarishta preparation followed the method as per Ayurvedic Formulary of India. Observations and Results: Analytical study of both the batches was done and their parameters were compared and analyzed including organoleptic features. Discussion: Both batches differ in the properties of final product as the sweetening agents used are different. Ashvagandhadyarishta containing guda as madhura dravya has greater values of analytical parameters comparatively, infers the influence of components present in guda. Conclusion: Change in the analytical and organoleptic characters were observed owing to the changes in the sweetening agents. Further research works has to be carried out to understand the clinical efficacy

Evaluation of Market Samples of Lauha Bhasma using Namburi Phased Spot Test (NPST)

Introduction: Lauha bhasma is a herbo- metallic preparation in Ayurveda; it possesses ruksha- guru- lekhana guna, madhura vipaka, sheeta veerya which is indicated in kushta, kshaya, pandu, krimi, etc. disease conditions. Though bhasma are prepared by following the classical methods, in this commercialized world its purity and quality is a question. So in this study, classical bhasma pareeksha and Namburi Phased Spot Test (NPST) methods were employed on two market samples of Lauha bhasma to assess their purity and quality. Materials and Methods: Lauha bhasma of both market samples (sample 1 and sample 2) were subjected to classical bhasma pareeksha and NPST test methods. Observations and Results: Both samples were subjected to NPST and different test methods then parameters obtained were compared to standard values as per CCRAS guidelines. Discussion: Both samples of Lauha bhasma have passed the classical bhasma pareeksha suggestive of proper formation ofbhasma but differed in the time period of changes observed at different phases in NPST test. pH of both samples signifies the alkaline nature of bhasma. Conclusion: Both samples have tested for different parameters in which sample 1 showed changes as mentioned in NPST standards on-time compared to sample 2.&nbsp

Effect of Vanillin on lipid profile in a model of hyperlipidemia, a preliminary study

288-291To evaluate the effect of vanillin on the lipid profile of high fat diet induced hyperlipidemia in rats, the hyperlipidemia was induced by feeding cholesterol-rich high fat diet for 45 days in wistar rats of either sex. The reduction in the triglycerides and VLDL-C was significant at 200 & 400 mg/kg dose of vanillin compared to atorvastatin group. Reduction in total cholesterol was significant at 200 and 400 mg/kg doses compared to hyperlipidemic control. The results demonstrate that vanillin at a dose of 200 and 400 mg/kg body weight lowers the serum triglyceride, VLDL-C and total cholesterol level significantly in high fat diet induced hyperlipidemic rats. However there was no significant effect on the lipid profile at 100 mg/kg dose. There were no statistically significant changes in the HDL-C and LDL-C levels at any of the given doses

Systematic computational toxicity analysis of the ozonolytic degraded compounds of azo dyes: Quantitative structure-activity relationship (QSAR) and adverse outcome pathway (AOP) based approach

International audienceThe present study identifies and analyses the degraded products of three azo dyes (Reactive Orange 16, Reactive Red 120, and Direct Red 80) and proffers their in silico toxicity predictions. In our previously published work, the synthetic dye effluents were degraded using an ozonolysis-based Advanced Oxidation Process. In the present study, the degraded products of the three dyes were analysed using GC-MS at endpoint strategy and further subjected to in silico toxicity analysis using Toxicity Estimation Software Tool (TEST), Prediction Of TOXicity of chemicals (ProTox-II), and Estimation Programs Interface Suite (EPI Suite). Several physiological toxicity endpoints, such as hepatotoxicity, carcinogenicity, mutagenicity, cellular and molecular interactions, were considered to assess the Quantitative Structure-Activity Relationships (QSAR) and adverse outcome pathways. The environmental fate of the by-products in terms of their biodegradability and possible bioaccumulation was also assessed. Results of ProTox-II suggested that the azo dye degradation products are carcinogenic, immunotoxic, and cytotoxic and displayed toxicity towards Androgen Receptor and Mitochondrial Membrane Potential. TEST results predicted LC50 and IGC50 values for three organisms Tetrahymena pyriformis, Daphnia magna, and Pimephales promelas. EPISUITE software via the BCFBAF module surmises that the degradation products' bioaccumulation (BAF) and bioconcentration factors (BCF) are high. The cumulative inference of the results suggests that most degradation by-products are toxic and need further remediation strategies. The study aims to complement existing tests to predict toxicity and prioritise the elimination/reduction of harmful degradation products of primary treatment procedures. The novelty of this study is that it streamlines in silico approaches to predict the nature of toxicity of degradation by-products of toxic industrial affluents like azo dyes. These approaches can assist the first phase of toxicology assessments for any pollutant for regulatory decision-making bodies to chalk out appropriate action plans for their remediation

3rd National Conference on Image Processing, Computing, Communication, Networking and Data Analytics

This volume contains contributed articles presented in the conference NCICCNDA 2018, organized by the Department of Computer Science and Engineering, GSSS Institute of Engineering and Technology for Women, Mysore, Karnataka (India) on 28th April 2018