Urinary leukotriene E4 at 12 months and influencing factors

Stencel-Gabriel K, Czuba Z, Gabriel I, Majda A. Urinary leukotriene E4 at 12 months and influencing factors. Journal of Education, Health and Sport. 2016;6(1):197-206. eISSN 2391-8306. DOI http://dx.doi.org/10.5281/zenodo.45337http://ojs.ukw.edu.pl/index.php/johs/article/view/45337https://pbn.nauka.gov.pl/works/709857   The journal has had 7 points in Ministry of Science and Higher Education parametric evaluation. Part B item 755 (23.12.2015).755 Journal of Education, Health and Sport eISSN 2391-8306 7© The Author (s) 2016; This article is published with open access at Licensee Open Journal Systems of Kazimierz Wielki University in Bydgoszcz, PolandOpen Access. This article is distributed under the terms of the Creative Commons Attribution Noncommercial License which permits any noncommercial use, distribution, and reproduction in any medium,provided the original author(s) and source are credited. This is an open access article licensed under the terms of the Creative Commons Attribution Non Commercial License(http://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted, non commercial use, distribution and reproduction in any medium, provided the work is properly cited.This is an open access article licensed under the terms of the Creative Commons Attribution Non Commercial License (http://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted, non commercialuse, distribution and reproduction in any medium, provided the work is properly cited.The authors declare that there is no conflict of interests regarding the publication of this paper.Received: 15.12.2015. Revised 12.01.2016. Accepted: 25.01.2016.  Urinary leukotriene E4 at 12 months and influencing factors Stencel-Gabriel K ( 1 ), Czuba Z ( 2 ), Gabriel I ( 3 ), Majda A ( 1 ) 1.        Departament of Pediatrics, Bytom, Medical University of Silesia2.        Departament of Microbiology and Immunology, Zabrze, Medical University of Silesia3.        Department of Gynecology, Obstetrics and Oncological Gynecology, Bytom, Medical University of Silesia   Corresponding author:Krystyna Stencel-Gabrielul. Batorego 1541-902 Bytom Keywords: leukotriene E4, infants, allergy, urine. SummaryObjectiveLTE4 is the end point of cysLTs pathway and its only stable product. Its role was discussed in asthma and AEDS.We aimed to investigate the impact of genetic and environmental factors ( sex, maternal positive family atopy history, breastfeeding, passive smoking and pet exposure ).60 newborns ( including 30 boys ) were enrolled in the study. Each child was examined at 12 months and urine samples for urinary LTE4 measurement were collected. All samples were processed using ACETM Enzyme Immunoassay Kit ( Cayman Chemical, Ann Arbor, MI, USA ).The mean level of urinary LTE4 at 12 months was 186,99 pg/ml ( median: 159,0; CI 95%: 157,79- 221,59 ). The Shapiro- Wilk test showed that the distribution of the levels of urinary LTE4 were abnormal. 12- month- old girls had higher urinary LTE4 levels than boys( mean: 270,50 vs. 193,55 ), but maternal positive atopy history, pet exposure, tobacco smoking or length of breastfeeding had no impact on urinary LTE4 excretion.ConclusionsIn conclusion, most of genetic or environmental factors do not change levels of urinary LTE4 in infants.

Experimental evaluation of sub-sampling IQ detection for low-level RF control in particle accelerator systems

The low-level radio frequency (LLRF) control system is one of the fundamental parts of a particle accelerator, ensuring the stability of the electro-magnetic (EM) field inside the resonant cavities. It leverages on the precise measurement of the field by in-phase/quadrature (IQ) detection of an RF probe signal from the cavities, usually performed using analogue downconversion. This approach requires a local oscillator (LO) and is subject to hardware non-idealities like mixer nonlinearity and long-term temperature drifts. In this work, we experimentally evaluate IQ detection by direct sampling for the LLRF system of the Polish free electron laser (PolFEL) now under development at the National Centre for Nuclear Research (NCBJ) in Poland. We study the impact of the sampling scheme and of the clock phase noise for a 1.3-GHz input sub-sampled by a 400-MSa/s analogue-to-digital converter (ADC), estimating amplitude and phase stability below 0.01% and nearly 0.01◦, respectively. The results are in line with state-of-the-art implementations, and demonstrate the feasibility of direct sampling for GHz-range LLRF systems

The Power of Environmental Observatories for Advancing Multidisciplinary Research, Outreach, and Decision Support: The Case of the Minnesota River Basin

An edited version of this paper was published by AGU. Copyright 2019 American Geophysical Union.Observatory‐scale data collection efforts allow unprecedented opportunities for integrative, multidisciplinary investigations in large, complex watersheds, which can affect management decisions and policy. Through the National Science Foundation‐funded REACH (REsilience under Accelerated CHange) project, in collaboration with the Intensively Managed Landscapes‐Critical Zone Observatory, we have collected a series of multidisciplinary data sets throughout the Minnesota River Basin in south‐central Minnesota, USA, a 43,400‐km2 tributary to the Upper Mississippi River. Postglacial incision within the Minnesota River valley created an erosional landscape highly responsive to hydrologic change, allowing for transdisciplinary research into the complex cascade of environmental changes that occur due to hydrology and land use alterations from intensive agricultural management and climate change. Data sets collected include water chemistry and biogeochemical data, geochemical fingerprinting of major sediment sources, high‐resolution monitoring of river bluff erosion, and repeat channel cross‐sectional and bathymetry data following major floods. The data collection efforts led to development of a series of integrative reduced complexity models that provide deeper insight into how water, sediment, and nutrients route and transform through a large channel network and respond to change. These models represent the culmination of efforts to integrate interdisciplinary data sets and science to gain new insights into watershed‐scale processes in order to advance management and decision making. The purpose of this paper is to present a synthesis of the data sets and models, disseminate them to the community for further research, and identify mechanisms used to expand the temporal and spatial extent of short‐term observatory‐scale data collection efforts

The Power of Environmental Observatories for Advancing Multidisciplinary Research, Outreach, and Decision Support: The Case of the Minnesota River Basin

Observatory‐scale data collection efforts allow unprecedented opportunities for integrative, multidisciplinary investigations in large, complex watersheds, which can affect management decisions and policy. Through the National Science Foundation‐funded REACH (REsilience under Accelerated CHange) project, in collaboration with the Intensively Managed Landscapes‐Critical Zone Observatory, we have collected a series of multidisciplinary data sets throughout the Minnesota River Basin in south‐central Minnesota, USA, a 43,400‐km2 tributary to the Upper Mississippi River. Postglacial incision within the Minnesota River valley created an erosional landscape highly responsive to hydrologic change, allowing for transdisciplinary research into the complex cascade of environmental changes that occur due to hydrology and land use alterations from intensive agricultural management and climate change. Data sets collected include water chemistry and biogeochemical data, geochemical fingerprinting of major sediment sources, high‐resolution monitoring of river bluff erosion, and repeat channel cross‐sectional and bathymetry data following major floods. The data collection efforts led to development of a series of integrative reduced complexity models that provide deeper insight into how water, sediment, and nutrients route and transform through a large channel network and respond to change. These models represent the culmination of efforts to integrate interdisciplinary data sets and science to gain new insights into watershed‐scale processes in order to advance management and decision making. The purpose of this paper is to present a synthesis of the data sets and models, disseminate them to the community for further research, and identify mechanisms used to expand the temporal and spatial extent of short‐term observatory‐scale data collection efforts

Effect of Flavone Derivatives on Interleukin‐1β (IL‐1β) mRNA Expression and IL‐1β Protein Synthesis in Stimulated RAW 264.7 Macrophages

It is known that the redox status of cells affects gene expression. Flavones, as natural antioxidants, efficiently modulate this status and may play a role in the regulation of inducible gene expression of inflammatory mediators. This study was designed to investigate the effect of five flavone derivatives variously substituted with hydroxyl groups (chrysin, galangin, kaempferol, quercetin and myricetin) on interleukin‐1β (IL‐1β) gene expression in stimulated RAW 264.7 macrophages. The cells were incubated with tested hydroxyflavones and stimulated with lipopolysaccharide (LPS) and interferon‐γ (IFN‐γ). Then, the following were estimated: the level of IL‐1β mRNA in these cells and the concentration of IL‐1β protein in cell‐culture supernatants and cell lysates. Each of the tested compounds significantly decreased IL‐1β mRNA expression. The most potent inhibitor was chrysin (hydroxyflavone with two hydroxyl groups and a weak antioxidant activity). The effects of galangin and kaempferol were similar. Myricetin (hydroxyflavone with a strong antioxidant activity) significantly decreased the level of IL‐1β mRNA, but it had no effect on the IL‐1β protein synthesis. The results indicated that hydroxyflavones could modulate the IL‐1β gene expression in activated RAW 264.7 macrophages via inhibiting gene transcription. This action seems unlikely to be the result of antioxidant properties of tested compounds

Saliva-Based Protein Analysis in Pediatric Dentofacial Inflammation

Bogusława Orzechowska-Wylęgała,1 Adam Wylęgała,2 Jolanta Zalejska Fiolka,3 Zenon Czuba,4 Michal Toborek5 1Department of Pediatric Otolaryngology, Head and Neck Surgery, Chair of Pediatric Surgery, Medical University of Silesia (SUM), Katowice, Poland; 2Health Promotion and Obesity Management, Department of Pathophysiology in Katowice, SUM, Katowice, Poland; 3Department of Biochemistry Faculty of Medical Science in Zabrze, SUM, Katowice, Poland; 4Department and Division of Microbiology and Immunology in Zabrze, SUM, Katowice, Poland; 5Department of Biochemistry and Molecular Biology, University of Miami, School of Medicine, Miami, FL, USACorrespondence: Bogusława Orzechowska-Wylęgała, Email [email protected]: Saliva contains various proteins that are important in developing inflammatory processes and their prevention. One key aspect of saliva research is the relationship between oral infections and inflammation, and the role of some salivary proteins.The Work Aims: To demonstrate which salivary cytokines can be biomarkers of acute odontogenic oral and facial infections in children.Material and Methods: The study included two groups of patients: a study group of 28 children: 7 girls and 21 boys aged 3 − 17 years with acute dentofacial inflammation (DI) and a control group of 52 children: 16 girls and 36 boys aged 4– 17 years with uncomplicated dental caries (CE). The levels of Interleukin-5 (IL-5), Interleukin − 10 (IL-10), Interleukin-17A (IL-17A), Interleukin-12p70 (IL-12p70), Eotaxin, Rantes, Vascular Endothelial Growth Factor (VEGF), and Interferon gamma-induced protein 10 (IP10) in the saliva of children in DI and CE groups were compared. Statistical analysis was performed with Statistica 13. The Student’s t-test and the Wilcoxon signed-rank test were used.Results: The results show that IL-10, IL-17A, and Eotaxin showed a statistically significant increase in the DI group compared to the CE group. The significance level for IL-10 was p=0.02, for IL-17A was equal to Eotaxin and p=0.04. The other measured parameters did not differ statistically significant between the two groups.Conclusion: IL-10, IL-17A, and Eotaxin can be used as potential biomarkers for tooth-related inflammatory states of the oral cavity and face in children. These biomarkers can be useful in identifying and monitoring the presence of inflammation in the oral cavity and face.Keywords: salivary cytokines and chemokines, odontogenic oral and facial inflammation, childre