Entwinement and the emergence of spacetime

It is conventional to study the entanglement between spatial regions of a quantum field theory. However, in some systems entanglement can be dominated by "internal", possibly gauged, degrees of freedom that are not spatially organized, and that can give rise to gaps smaller than the inverse size of the system. In a holographic context, such small gaps are associated to the appearance of horizons and singularities in the dual spacetime. Here, we propose a concept of entwinement, which is intended to capture this fine structure of the wavefunction. Holographically, entwinement probes the entanglement shadow -- the region of spacetime not probed by the minimal surfaces that compute spatial entanglement in the dual field theory. We consider the simplest example of this scenario -- a 2d conformal field theory (CFT) that is dual to a conical defect in AdS3 space. Following our previous work, we show that spatial entanglement in the CFT reproduces spacetime geometry up to a finite distance from the conical defect. We then show that the interior geometry up to the defect can be reconstructed from entwinement that is sensitive to the discretely gauged, fractionated degrees of freedom of the CFT. Entwinement in the CFT is related to non-minimal geodesics in the conical defect geometry, suggesting a potential quantum information theoretic meaning for these objects in a holographic context. These results may be relevant for the reconstruction of black hole interiors from a dual field theory.Comment: v2: Sec. 4.3 amende

The entropy of a hole in spacetime

We compute the gravitational entropy of 'spherical Rindler space', a time-dependent, spherically symmetric generalization of ordinary Rindler space, defined with reference to a family of observers traveling along non-parallel, accelerated trajectories. All these observers are causally disconnected from a spherical region H (a 'hole') located at the origin of Minkowski space. The entropy evaluates to S = A/4G, where A is the area of the spherical acceleration horizon, which coincides with the boundary of H. We propose that S is the entropy of entanglement between quantum gravitational degrees of freedom supporting the interior and the exterior of the sphere H.Comment: 9 pages, 1 figure; v2: published version including updated reference

A hole-ographic spacetime

We embed spherical Rindler space -- a geometry with a spherical hole in its center -- in asymptotically AdS spacetime and show that it carries a gravitational entropy proportional to the area of the hole. Spherical AdS-Rindler space is holographically dual to an ultraviolet sector of the boundary field theory given by restriction to a strip of finite duration in time. Because measurements have finite durations, local observers in the field theory can only access information about bounded spatial regions. We propose a notion of Residual Entropy that captures uncertainty about the state of a system left by the collection of local, finite-time observables. For two-dimensional conformal field theories we use holography and the strong subadditivity of entanglement to propose a formula for Residual Entropy and show that it precisely reproduces the areas of circular holes in AdS3. Extending the notion to field theories on strips with variable durations in time, we show more generally that Residual Entropy computes the areas of all closed, inhomogenous curves on a spatial slice of AdS3. We discuss the extension to higher dimensional field theories, the relation of Residual Entropy to entanglement between scales, and some implications for the emergence of space from the RG flow of entangled field theories.Comment: v3: minor typos correcte

In situ measurements of HCN and CH3CN over the Pacific Ocean: Sources, sinks, and budgets

We report the first in situ measurements of hydrogen cyanide (HCN) and methyl cyanide (CH3CN, acetonitrile) from the Pacific troposphere (0-12 km) obtained during the NASA Transport and Chemical Evolution over the Pacific (TRACE-P) airborne mission (February-April 2001). Mean HCN and CH3CN mixing ratios of 243 ± 118 (median 218) ppt and 149 ± 56 (median 138) ppt, respectively, were measured. These in situ observations correspond to a mean tropospheric HCN column of 4.2 × 1015 molecules cm-2 and a CH3CN column of 2.5 × 1015 molecules cm-2. This is in good agreement with the 0-12 km HCN column of 4.4 (±0.6) × 1015 molecules cm-2 derived from infrared solar spectroscopic observations over Japan. Mixing ratios of HCN and CH3CN were greatly enhanced in pollution outflow from Asia and were well correlated with each other as well as with known tracers of biomass combustion (e.g., CH3Cl, CO). Volumetric enhancement (or emission) ratios (ERs) relative to CO in free tropospheric plumes, likely originating from fires, were 0.34% for HCN and 0.17% for CH3CN. ERs with respect to CH3Cl and CO in selected biomass burning (BB) plumes in the free troposphere and in boundary layer pollution episodes are used to estimate a global BB source of 0.8 ± 0.4 Tg (N) yr-1 for HCN and 0.4 ± 0.1 Tg (N) yr-1 for CH3CN. In comparison, emissions from industry and fossil fuel combustion are quite small (<0.05 Tg (N) yr-1). The vertical structure of HCN and CH3CN indicated reduced mixing ratios in the marine boundary layer (MBL). Using a simple box model, the observed gradients across the top of the MBL are used to derive an oceanic loss rate of 8.8 × 10-15 g (N) cm-2 s-1 for HCN and 3.4 × 10-15 g (N) cm-2 s-1 for CH3CN. An air-sea exchange model is used to conclude that this flux can be maintained if the oceans are undersaturated in HCN and CH3CN by 27% and 6%, respectively. These observations also correspond to an open ocean mean deposition velocity (vd) of 0.12 cm s-1 for HCN and 0.06 cm s-1 for CH3CN. It is inferred that oceanic loss is a dominant sink for these cyanides and that they deposit some 1.4 Tg (N) of nitrogen annually to the oceans. Assuming loss to the oceans and reaction with OH radicals as the major removal processes, a mean atmospheric residence time of 5.0 months for HCN and 6.6 months for CH3CN is calculated. A global budget analysis shows that the sources and sinks of HCN and CH3CN are roughly in balance but large uncertainties remain in part due to a lack of observational data from the atmosphere and the oceans. Pathways leading to the oceanic (and soil) degradation of these cyanides are poorly known but are expected to be biological in nature

Loop-mediated isothermal amplification (LAMP) assays for the species-specific detection of Eimeria that infect chickens

<p>Abstract</p> <p>Background</p> <p><it>Eimeria </it>parasites can cause the disease coccidiosis in poultry and even subclinical infection can incur economic loss. Diagnosis of infection predominantly relies on traditional techniques including lesion scoring and faecal microscopy despite the availability of sensitive molecular assays, largely due to cost and the requirement for specialist equipment. Despite longstanding proven efficacy these traditional techniques demand time and expertise, can be highly subjective and may under-diagnose subclinical disease. Recognition of the tight economic margins prevailing in modern poultry production and the impact of avian coccidiosis on poverty in many parts of the world has highlighted a requirement for a panel of straightforward and sensitive, but cost-effective, <it>Eimeria </it>species-specific diagnostic assays.</p> <p>Results</p> <p>Loop-mediated isothermal amplification (LAMP) is an uncomplicated, quick and relatively inexpensive diagnostic tool. In this study we have developed a panel of species-specific LAMP assays targeting the seven <it>Eimeria </it>species that infect the chicken. Each assay has been shown to be genuinely species-specific with the capacity to detect between one and ten eimerian genomes, equivalent to less than a single mature schizont. Development of a simple protocol for template DNA preparation from tissue collected post mortem with no requirement for specialist laboratory equipment supports the use of these assays in routine diagnosis of eimerian infection. Preliminary field testing supports this hypothesis.</p> <p>Conclusions</p> <p>Development of a panel of sensitive species-specific LAMP assays introduces a valuable new cost-effective tool for use in poultry husbandry.</p

Analysis of the atmospheric distribution, sources, and sinks of oxygenated volatile organic chemicals based on measurements over the Pacific during TRACE-P

Airborne measurements of a large number of oxygenated volatile organic chemicals (OVOC) were carried out in the Pacific troposphere (0.1 - 12 km) in winter/spring of 2001 (24 February to 10 April). Specifically, these measurements included acetone (CH3COCHA3), methylethyl ketone (CH3COC2H5, MEK), methanol (CH3OH), ethanol (C2H5OH), acetaldehyde (CH3CHO), propionaldehyde C2H 5CHO), peroxyacylnitrates (PANs) (CnH 2n+1COO2NO2), and organic nitrates (CnH2n+1ONO2). Complementary measurements of formaldehyde (HCHO), methyl hydroperoxide (CH 3OOH), and selected tracers were also available. OVOC were abundant in the clean troposphere and were greatly enhanced in the outflow regions from Asia. Background mixing ratios were typically highest in the lower troposphere and declined toward the upper troposphere and the lowermost stratosphere. Their total abundance (ΣOVOC) was nearly twice that of nonmethane hydrocarbons (Σ C2-C8 NMHC. Throughout the troposphere, the OH reactivity of OVOC is comparable to that of methane and far exceeds that of NMHC. A comparison of these data with western Pacific observations collected some 7 years earlier (February-March 1994) did not reveal significant differences. Mixing ratios of OVOC were strongly correlated with each other as well as with tracers of fossil and biomass/biofuel combustion. Analysis of the relative enhancement of selected OVOC with respect to CH 3Cl and CO in 12 plumes originating from fires and sampled in the free troposphere (3-11 km) is used to assess their primary and secondary emissions from biomass combustion. The composition of these plumes also indicates a large shift of reactive nitrogen into the PAN reservoir thereby limiting ozone formation. A three-dimensional global model that uses state of the art chemistry and source information is used to compare measured and simulated mixing ratios of selected OVOC. While there is reasonable agreement in many cases, measured aldehyde concentrations are significantly larger than predicted. At their observed levels, acetaldehyde mixing ratios are shown to be an important source of HCHO (and HOx) and PAN in the troposphere. On the basis of presently known chemistry, measured mixing ratios of aldehydes and PANs are mutually incompatible. We provide rough estimates of the global sources of several OVOC and conclude that collectively these are extremely large (150-500 Tg C yr-1) but remain poorly quantified. Copyright 2004 by the American Geophysical Union

Immunotherapy for Infarcts: In Vivo Postinfarction Macrophage Modulation Using Intramyocardial Microparticle Delivery of Map4k4 Small Interfering RNA

The myeloid cells infiltrating the heart early after acute myocardial infarction elaborate a secretome that largely orchestrates subsequent ventricular wall repair. Regulating this innate immune response could be a means to improve infarct healing. To pilot this concept, we utilized (beta1,3-d-) glucan-encapsulated small interfering RNA (siRNA)-containing particles (GeRPs), targeting mononuclear phagocytes, delivered to mice as a one-time intramyocardial injection immediately after acute infarction. Findings demonstrated that cardiac macrophages phagocytosed GeRPs in vivo and had little systemic dissemination, thus providing a means to deliver local therapeutics. Acute infarcts were then injected in vivo with phosphate-buffered saline (PBS; vehicle) or GeRPs loaded with siRNA to Map4k4, and excised hearts were examined at 3 and 7 days by quantitative polymerase chain reaction, flow cytometry, and histology. Compared with infarcted PBS-treated hearts, hearts with intrainfarct injections of siRNA-loaded GeRPs exhibited 69-89% reductions in transcripts for Map4k4 (mitogen-activated protein kinase kinase kinase kinase 4), interleukin (IL)-1beta, and tumor necrosis factor alpha at 3 days. Expression of other factors relevant to matrix remodeling-monocyte chemoattractant protein-1 (MCP-1), matrix metalloproteinases, hyaluronan synthases, matricellular proteins, and profibrotic factors transforming growth factor beta (TGF-beta), and connective tissue growth factor (CTGF)-were also decreased. Most effects peaked at 3 days, but, in some instances (Map4k4, IL-1beta, TGF-beta, CTGF, versican, and periostin), suppression persisted to 7 days. Thus, direct intramyocardial GeRP injection could serve as a novel and clinically translatable platform for in vivo RNA delivery to intracardiac macrophages for local and selective immunomodulation of the infarct microenvironment