Rediscovery of Ooline, Cadellia pentastylis, near Gunnedah : notes on the habitat and ecology of this dry rainforest tree

Cadellia pentastylis F.Muell., (family Surianaceae), a dry rainforest tree with a conservation listing of Vulnerable at state and national levels, was first collected from the Gunnedah area by the botanical collector J.L. Boorman in 1907. We report the first record of Cadellia pentastylis from the Gunnedah area (30°58’49’’S, 150°15’15’’E) since 1907, and provide details of the community and habitat where it occurs, on the lower slopes of Black Jack Mountain. Although this population is one of the smallest in New South Wales, it is significant as it is at its southern distributional limit, and is found adjacent to semi-evergreen vine thicket, another type of dry rainforest, on the same hillslope. We list the New South Wales occurrences of this species and discuss aspects of its flowering phenology

A broad typology of dry rainforests on the western slopes of New South Wales

Dry rainforests are those communities that have floristic and structural affinities to mesic rainforests and occur in parts of eastern and northern Australia where rainfall is comparatively low and often highly seasonal. The dry rainforests of the western slopes of New South Wales are poorly-understood compared to other dry rainforests in Australia, due to a lack of regional scale studies. This paper attempts to redress this by deriving a broad floristic and structural typology for this vegetation type. Phytogeographical analysis followed full floristic surveys conducted on 400 m2 plots located within dry rainforest across the western slopes of NSW. Cluster analysis and ordination of 208 plots identified six floristic groups. Unlike in some other regional studies of dry rainforest these groups were readily assigned to Webb structural types, based on leaf size classes, leaf retention classes and canopy height. Five community types were described using both floristic and structural data: 1) Ficus rubiginosa–Notelaea microcarpa notophyll vine thicket, 2) Ficus rubiginosa–Alectryon subcinereus–Notelaea microcarpa notophyll vine forest, 3) Elaeodendron australe–Notelaea microcarpa–Geijera parviflora notophyll vine thicket, 4) Notelaea microcarpa– Geijera parviflora–Ehretia membranifolia semi-evergreen vine thicket, and 5) Cadellia pentastylis low microphyll vine forest. Floristic groupings were consistent with those described by previous quantitative studies which examined smaller portions of this study area. There was also general agreement between the present analytical study and a previous intuitive classification of dry rainforest vegetation throughout the study area, but little concurrence with a continental scale floristic classification of rainforest

MARQUIS: A multiplex method for absolute quantification of peptides and posttranslational modifications

Absolute quantification of protein expression and posttranslational modifications by mass spectrometry has been challenging due to a variety of factors, including the potentially large dynamic range of phosphorylation response. To address these issues, we have developed MARQUIS—Multiplex Absolute Regressed Quantification with Internal Standards—a novel mass spectrometry-based approach using a combination of isobaric tags and heavy-labelled standard peptides, to construct internal standard curves for peptides derived from key nodes in signal transduction networks. We applied MARQUIS to quantify phosphorylation dynamics within the ​EGFR network at multiple time points following stimulation with several ligands, enabling a quantitative comparison of ​EGFR phosphorylation sites and demonstrating that receptor phosphorylation is qualitatively similar but quantitatively distinct for each ​EGFR ligand tested. MARQUIS was also applied to quantify the effect of ​EGFR kinase inhibition on glioblastoma patient-derived xenografts. MARQUIS is a versatile method, broadly applicable and extendable to multiple mass spectrometric platforms.United States-Israel Binational Science FoundationNational Institutes of Health (U.S.) (Grant U54 CA112967)National Institutes of Health (U.S.) (Grant R01 CA118705)National Institutes of Health (U.S.) (Grant R01 CA096504)Mayo Brain Tumor SPORE CA10896

Computer aided manual validation of mass spectrometry-based proteomic data

Advances in mass spectrometry-based proteomic technologies have increased the speed of analysis and the depth provided by a single analysis. Computational tools to evaluate the accuracy of peptide identifications from these high-throughput analyses have not kept pace with technological advances; currently the most common quality evaluation methods are based on statistical analysis of the likelihood of false positive identifications in large-scale data sets. While helpful, these calculations do not consider the accuracy of each identification, thus creating a precarious situation for biologists relying on the data to inform experimental design. Manual validation is the gold standard approach to confirm accuracy of database identifications, but is extremely time-intensive. To palliate the increasing time required to manually validate large proteomic datasets, we provide computer aided manual validation software (CAMV) to expedite the process. Relevant spectra are collected, catalogued, and pre-labeled, allowing users to efficiently judge the quality of each identification and summarize applicable quantitative information. CAMV significantly reduces the burden associated with manual validation and will hopefully encourage broader adoption of manual validation in mass spectrometry-based proteomics.National Institutes of Health (U.S.) (Grant R24DK090963)National Institutes of Health (U.S.) (Grant U54CA112967)National Cancer Institute (U.S.). Integrative Cancer Biology Program (Fellowship)Charles S. Krakauer FellowshipHugh Hampton Young Fellowshi

Reducing Substance Abuse Stigma in Employment Application

A history of substance use and having a history of legal charges can be a barrier to employment. Available research shows individuals with a criminal history of substance related charges have greater difficulty obtaining employment due to the presence of substance abuse stigma and criminal history stigma. Research also shows that employers with higher levels of fundamentalism and conservativism are more likely to negatively evaluate applicants with a criminal history of substance charges. Furthermore, available research demonstrates employers in rural areas are more likely to deny employment to an individual with a legal history of substance charges based upon higher rates of familiarity within the community. Stigma research shows personal contact with a stigmatized population can reduce stigma. This study set out to determine if changing the response on the legal history section of an employment application would have an effect on mock employer’s evaluations of job applications. In order to examine this, 458 participants were randomly assigned to read an employment application in one of three conditions. The only difference in the applications was the legal history section, which varied as “none,” “possession of a controlled substance,” or “will discuss.” Participants also completed measures of substance use stigma, conservativism, and fundamentalism. Further, participants’ geographic region (rural vs. non-rural) was examined. Results showed the only significant predictor of not granting an interview was the presence of a legal charge. The findings demonstrated listing ‘will discuss’ on the legal history section reduces the chances of obtaining an interview. Implications of the current study indicate that personal values or rural status should not be a main focus of intervention when trying to reduce stigma for individuals with a legal history of substance charges. Limitations of the study include lack of a robust manipulation check and limited risk in granting an interview. Future studies should focus on research designs that incorporate a limited number of available interview opportunities, and examine whether other legal charges would have similar effects in employment application

THIRD PARTY EFFECTS OF AFFECTIONATE COMMUNICATION IN FAMILY SUBSYSTEMS: EXAMINING INFLUENCE ON AFFECTIONATE COMMUNICATION, MENTAL WELL-BEING, AND FAMILY SATISFACTION

This study examined the links between affectionate communication expressed within family dyads and affectionate communication expressed among other dyads, as well as individual reports of satisfaction with family life and mental well-being. Overall, the study showed that a child’s report of affectionate communication exchanged in the child/father subsystem is associated with mother’s satisfaction with family life. Additionally, mother reports of affectionate communication exchanged in the spousal relationships were positively associated with child reports of child/father affectionate communication exchanged. Finally, both perceptions of affectionate exchange in dyads outside and inside of one’s direct experience correlated with satisfaction with family life and mental well-being. The results offer new insights into the nature of affectionate relationships in families