The Complete Mitogenome of the Mountain Chicken Frog, Leptodactylus fallax

The mountain chicken frog (Leptodactylus fallax) is a critically endangered frog native to the Caribbean islands of Dominica and Montserrat. Over the past 25 years their populations have declined by over 85%, largely due to a chytridiomycosis outbreak that nearly wiped out the Montserratian population. Within the context of developing tools that can aid in the conservation of the mountain chicken frog, we assembled its complete mitochondrial genome, contributing the first complete mitogenome of the genus Leptodactylus (Genbank Accession number MW260634). The circular genome is 18,669 bp long and contains 37 genes. A phylogenetic analysis reveals that L. fallax forms a clade with Leptodactylus melanonotus, highlighting the close relationship of Leptodactylus spp. relative to other species from the superfamily Hyloidea included in the analysis

The Complete Mitogenome of the Mountain Chicken Frog, Leptodactylus fallax

The mountain chicken frog (Leptodactylus fallax) is a critically endangered frog native to the Caribbean islands of Dominica and Montserrat. Over the past 25 years their populations have declined by over 85%, largely due to a chytridiomycosis outbreak that nearly wiped out the Montserratian population. Within the context of developing tools that can aid in the conservation of the mountain chicken frog, we assembled its complete mitochondrial genome, contributing the first complete mitogenome of the genus Leptodactylus (Genbank Accession number MW260634). The circular genome is 18,669 bp long and contains 37 genes. A phylogenetic analysis reveals that L. fallax forms a clade with Leptodactylus melanonotus, highlighting the close relationship of Leptodactylus spp. relative to other species from the superfamily Hyloidea included in the analysis

Detecting 2-joins faster

2-joins are edge cutsets that naturally appear in the decomposition of several classes of graphs closed under taking induced subgraphs, such as balanced bipartite graphs, even-hole-free graphs, perfect graphs and claw-free graphs. Their detection is needed in several algorithms, and is the slowest step for some of them. The classical method to detect a 2-join takes $O(n^3m)$ time where $n$ is the number of vertices of the input graph and $m$ the number of its edges. To detect \emph{non-path} 2-joins (special kinds of 2-joins that are needed in all of the known algorithms that use 2-joins), the fastest known method takes time $O(n^4m)$. Here, we give an $O(n^2m)$-time algorithm for both of these problems. A consequence is a speed up of several known algorithms

Dynamic Transition Theory for Thermohaline Circulation

The main objective of this and its accompanying articles is to derive a mathematical theory associated with the thermohaline circulations (THC). This article provides a general transition and stability theory for the Boussinesq system, governing the motion and states of the large-scale ocean circulation. First, it is shown that the first transition is either to multiple steady states or to oscillations (periodic solutions), determined by the sign of a nondimensional parameter $K$, depending on the geometry of the physical domain and the thermal and saline Rayleigh numbers. Second, for both the multiple equilibria and periodic solutions transitions, both Type-I (continuous) and Type-II (jump) transitions can occur, and precise criteria are derived in terms of two computable nondimensional parameters $b_1$ and $b_2$. Associated with Type-II transitions are the hysteresis phenomena, and the physical reality is represented by either metastable states or by a local attractor away from the basic solution, showing more complex dynamical behavior. Third, a convection scale law is introduced, leading to an introduction of proper friction terms in the model in order to derive the correct circulation length scale. In particular, the dynamic transitions of the model with the derived friction terms suggest that the THC favors the continuous transitions to stable multiple equilibria. Applications of the theoretical analysis and results to different flow regimes will be explored in the accompanying articles

Accelerating Primary Care Transformation at Jefferson (JeffAPCT): Reflections from a Five- Year HRSA Grant

JeffAPCT Overview Five year HRSA-funded grant (7/1/15-6/30/20) Leadership team from Family Medicine, Internal Medicine, Physician Assistant Program Objective 1: To improve/ expand primary care and population health curriculum across the continuum of primary care providers and trainees (students, residents, and practitioners) Objective 2: To create an enhanced, sustainable model of primary care physician faculty development for PCMH Transformation Objective 3: To create a new, sustainable model of faculty development for community-based primary care preceptors (MD/DO, PA, NP, others

Killer acquisitions

This paper argues incumbent firms may acquire innovative targets solely to discontinue the target’s innovation projects and preempt future competition. We call such acquisitions “killer acquisitions.” We develop a model illustrating this phenomenon. Using pharmaceutical industry data, we show that acquired drug projects are less likely to be developed when they overlap with the acquirer’s existing product portfolio, especially when the acquirer’s market power is large due to weak competition or distant patent expiration. Conservative estimates indicate 5.3 percent to 7.4 percent of acquisitions in our sample are killer acquisitions. These acquisitions disproportionately occur just below thresholds for antitrust scrutiny

Woodland birds and rural towns: artificial clutch survival in fragmented Box-Ironbark forests

Woodland birds are declining throughout the agricultural landscapes of south-eastern Australia, but the specific mechanisms driving these declines remain unclear. Reproductive failure via clutch depredation could conceivably contribute to these declines. Although site-scale habitat may influence the risk of clutch failure, larger-scale influences, such as whether a landscape contains a rural town or not (‘landscape type’), may also play a role. This study monitored artificial open-cup nests deployed in three pairs of the two landscape types and: 1) indexed clutch survival and predator assemblage; and 2) determined if clutch survival was influenced by landscape type and/or local habitat characteristics. High levels of clutch depredation were observed in both landscape types and for all landscapes, with no evidence to suggest that landscape type or habitat characteristics influenced clutch survival or the time-to-first-predator visit. Predator assemblage also was consistent between landscape types. Generalist avian predators were the most common egg predators. Such egg predators may be ubiquitous throughout the fragmented Box-Ironbark woodlands of south-eastern Australia

The thoughtful self

The relationship between a concept in the external world (e.g., the self), and its representation in cognition

Evidence for directional selection at a novel major histocompatibility class I marker in wild common frogs (Rana temporaria) exposed to a viral pathogen (Ranavirus).

(c) 2009 Teacher et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.Whilst the Major Histocompatibility Complex (MHC) is well characterized in the anuran Xenopus, this region has not previously been studied in another popular model species, the common frog (Rana temporaria). Nor, to date, have there been any studies of MHC in wild amphibian host-pathogen systems. We characterise an MHC class I locus in the common frog, and present primers to amplify both the whole region, and specifically the antigen binding region. As no more than two expressed haplotypes were found in over 400 clones from 66 individuals, it is likely that there is a single class I locus in this species. This finding is consistent with the single class I locus in Xenopus, but contrasts with the multiple loci identified in axolotls, providing evidence that the diversification of MHC class I into multiple loci likely occurred after the Caudata/Anura divergence (approximately 350 million years ago) but before the Ranidae/Pipidae divergence (approximately 230 mya). We use this locus to compare wild populations of common frogs that have been infected with a viral pathogen (Ranavirus) with those that have no history of infection. We demonstrate that certain MHC supertypes are associated with infection status (even after accounting for shared ancestry), and that the diseased populations have more similar supertype frequencies (lower F(ST)) than the uninfected. These patterns were not seen in a suite of putatively neutral microsatellite loci. We interpret this pattern at the MHC locus to indicate that the disease has imposed selection for particular haplotypes, and hence that common frogs may be adapting to the presence of Ranavirus, which currently kills tens of thousands of amphibians in the UK each year

Paradigm shift in public administration: Implications for teaching in professional training programs

10.1111/j.1540-6210.2009.02085.xPublic Administration Review69SUPPL.