PREY AVAILABILITY AND SELECTION BY MOUNTAIN LIONS IN ARAVAIPA-KLONDYKE AREA OF ARIZONA

Prey selection by mountain lions (Felis concolor) in the Aravaipa-Klondyke area (2,000 km) in southeastern Arizona was studied from February 1991 through September 1993. Overall diet from frequency of occurrence as determined from 370 scats was: 48% deer (white-tailed and mule deer combined), 34% cattle, 17% javelinas, 6% rabbit (cottontail and jackrabbit), 4% rodent, and 2% desert bighorn. Using a correction factor developed by Ackerman et al. (1984), we also estimated percent biomass and proportion of individuals killed. With respect to biomass consumed, cattle was 44%, deer 40%, javelinas 10.9%, rabbits 2.9%, and rodents 0.02%. Based on weights of prey consumed, proportion of individuals eaten changed to rabbits 52.7%, deer 16.3%, rodents 12%, javelinas 10% cattle 8%, and desert bighorn 0.5% Comparisons with availability as determined from four separate four-day double-count helicopter surveys found that lions selected calves (ate more than expected based on availability alone), killed and ate less deer than expected, and javelinas as would be expected

PREY AVAILABILITY AND SELECTION BY MOUNTAIN LIONS IN ARAVAIPA-KLONDYKE AREA OF ARIZONA

Prey selection by mountain lions (Felis concolor) in the Aravaipa-Klondyke area (2,000 km) in southeastern Arizona was studied from February 1991 through September 1993. Overall diet from frequency of occurrence as determined from 370 scats was: 48% deer (white-tailed and mule deer combined), 34% cattle, 17% javelinas, 6% rabbit (cottontail and jackrabbit), 4% rodent, and 2% desert bighorn. Using a correction factor developed by Ackerman et al. (1984), we also estimated percent biomass and proportion of individuals killed. With respect to biomass consumed, cattle was 44%, deer 40%, javelinas 10.9%, rabbits 2.9%, and rodents 0.02%. Based on weights of prey consumed, proportion of individuals eaten changed to rabbits 52.7%, deer 16.3%, rodents 12%, javelinas 10% cattle 8%, and desert bighorn 0.5% Comparisons with availability as determined from four separate four-day double-count helicopter surveys found that lions selected calves (ate more than expected based on availability alone), killed and ate less deer than expected, and javelinas as would be expected

The COVID-19 pandemic and obsessive-compulsive disorder in young people: Systematic review

BACKGROUND: The COVID-19 pandemic has impacted the world since the first cases were reported in China in January 2020. The secondary mental health impacts of the pandemic are thought to be significant. Obsessive-compulsive disorder is a condition defined by recurrent obsessions and compulsions. It has been hypothesised that the focus on hygiene and contamination during the pandemic could exacerbate obsessive-compulsive symptoms in young people. METHOD: A systematic literature review was conducted. Papers were sought looking at the effect of the pandemic on obsessive-compulsive disorder in young people. RESULTS: Six published cross-sectional and longitudinal studies were identified, of which four studies investigated clinic samples with a diagnosis of obsessive-compulsive disorder and two looked at community adolescent populations. Five out of the six studies found that obsessive-compulsive symptoms were exacerbated during the COVID-19 pandemic. CONCLUSION: The COVID-19 pandemic appears to be associated with a worsening of obsessive-compulsive symptoms in young people. Being in treatment seems to have a protective effect. Maintaining mental health services during a pandemic is vital. It is important to be aware of the implications of pandemic on obsessive-compulsive symptoms in young people in order to allow them to access appropriate treatments. More research is needed in this area

Low-loss flake-graphene saturable absorber mirror for laser mode-locking at sub-200-fs pulse duration

Saturable absorbers are a key component for mode-locking femtosecond lasers. Polymer films containing graphene flakes have recently been used in transmission as laser mode-lockers, but suffer from high nonsaturable loss, limiting their application in low-gain lasers. Here we present a saturable absorber mirror based on a film of pure graphene flakes. The device is used to mode lock an erbium-doped fiber laser, generating pulses with state-of-the-art, sub-200-fs duration. The laser characteristic indicate that the film exhibits low nonsaturable loss (13% per pass) and large absorption modulation depth (45% of low-power absorption)

Reducing Eating Disorder Onset in a Very High Risk Sample With Significant Comorbid Depression: A Randomized Controlled Trial

Objective: Eating disorders (EDs) are serious problems among college-age women and may be preventable. An indicated online eating disorder (ED) intervention, designed to reduce ED and comorbid pathology, was evaluated. Method: 206 women (M age = 20 +/- 1.8 years; 51% White/Caucasian, 11% African American, 10% Hispanic, 21% Asian/Asian American, 7% other) at very high risk for ED onset (i.e., with high weight/shape concerns plus a history of being teased, current or lifetime depression, and/or nonclinical levels of compensatory behaviors) were randomized to a 10-week, Internet-based, cognitive-behavioral intervention or waitlist control. Assessments included the Eating Disorder Examination (EDE, to assess ED onset), EDE-Questionnaire, Structured Clinical Interview for DSM Disorders, and Beck Depression Inventory-II. Results: ED attitudes and behaviors improved more in the intervention than control group (p = .02, d = 0.31); although ED onset rate was 27% lower, this difference was not significant (p = .28, NNT = 15). In the subgroup with highest shape concerns, ED onset rate was significantly lower in the intervention than control group (20% vs. 42%, p = .025, NNT = 5). For the 27 individuals with depression at baseline, depressive symptomatology improved more in the intervention than control group (p = .016, d = 0.96); although ED onset rate was lower in the intervention than control group, this difference was not significant (25% vs. 57%, NNT = 4). Conclusions: An inexpensive, easily disseminated intervention might reduce ED onset among those at highest risk. Low adoption rates need to be addressed in future research

Climate change promotes parasitism in a coral symbiosis.

Coastal oceans are increasingly eutrophic, warm and acidic through the addition of anthropogenic nitrogen and carbon, respectively. Among the most sensitive taxa to these changes are scleractinian corals, which engineer the most biodiverse ecosystems on Earth. Corals' sensitivity is a consequence of their evolutionary investment in symbiosis with the dinoflagellate alga, Symbiodinium. Together, the coral holobiont has dominated oligotrophic tropical marine habitats. However, warming destabilizes this association and reduces coral fitness. It has been theorized that, when reefs become warm and eutrophic, mutualistic Symbiodinium sequester more resources for their own growth, thus parasitizing their hosts of nutrition. Here, we tested the hypothesis that sub-bleaching temperature and excess nitrogen promotes symbiont parasitism by measuring respiration (costs) and the assimilation and translocation of both carbon (energy) and nitrogen (growth; both benefits) within Orbicella faveolata hosting one of two Symbiodinium phylotypes using a dual stable isotope tracer incubation at ambient (26 °C) and sub-bleaching (31 °C) temperatures under elevated nitrate. Warming to 31 °C reduced holobiont net primary productivity (NPP) by 60% due to increased respiration which decreased host %carbon by 15% with no apparent cost to the symbiont. Concurrently, Symbiodinium carbon and nitrogen assimilation increased by 14 and 32%, respectively while increasing their mitotic index by 15%, whereas hosts did not gain a proportional increase in translocated photosynthates. We conclude that the disparity in benefits and costs to both partners is evidence of symbiont parasitism in the coral symbiosis and has major implications for the resilience of coral reefs under threat of global change

A multi-institutional analysis of sternoclavicular joint coverage following osteomyelitis

BACKGROUND: Sternoclavicular joint (SCJ) osteomyelitis is a rare pathology requiring urgent intervention. Several operative approaches have been described with conflicting reports. Here, we present a multi-institutional study utilizing multiple surgical pathways for SCJ reconstruction. METHODS: A multi-institutional retrospective cohort study was conducted to identify patients who underwent surgical repair for sternoclavicular osteomyelitis between 2008 and 2019. Patients were stratified according to reconstruction approach: single-stage reconstruction with advancement flap and delayed-reconstruction with flap following initial debridement. Demographics, operative approach, type of reconstruction, and postoperative outcomes were analyzed. RESULTS: Thirty-two patients were identified. Mean patient age was 56.2±13.8 years and 68.8% were male. The average body mass index (BMI) was 30.0±8.8 kg/m2. The most common infection etiologies were intravenous drug use and bacteremia (both 25%). Fourteen patients (43.8%) underwent one-stage reconstruction and 18 (56.2%) underwent delayed twostaged reconstruction. Both single and delayed-stage groups had comparable rates of reinfection (7.1% vs. 11.1%, respectively), surgical site complications (21.4% vs. 27.8%), readmissions (7.1% vs. 16.6%), and reoperations (7.1% vs. 5.6%; all P\u3e0.05). The single-stage reconstruction group had a significantly lower BMI (26.2±5.7 kg/m2 vs. 32.9±9.1 kg/m2; P CONCLUSIONS: Both single and delayed-stage approaches are appropriate methods with comparable outcomes for reconstruction for SCJ osteomyelitis. When clinically indicated, a single-stage reconstruction approach may be preferable in order to avoid a second operation as associated with the delayed phase, and possibly shortening total hospital length of stay

The NlpD Lipoprotein Is a Novel Yersinia pestis Virulence Factor Essential for the Development of Plague

Yersinia pestis is the causative agent of plague. Previously we have isolated an attenuated Y. pestis transposon insertion mutant in which the pcm gene was disrupted. In the present study, we investigated the expression and the role of pcm locus genes in Y. pestis pathogenesis using a set of isogenic surE, pcm, nlpD and rpoS mutants of the fully virulent Kimberley53 strain. We show that in Y. pestis, nlpD expression is controlled from elements residing within the upstream genes surE and pcm. The NlpD lipoprotein is the only factor encoded from the pcm locus that is essential for Y. pestis virulence. A chromosomal deletion of the nlpD gene sequence resulted in a drastic reduction in virulence to an LD50 of at least 107 cfu for subcutaneous and airway routes of infection. The mutant was unable to colonize mouse organs following infection. The filamented morphology of the nlpD mutant indicates that NlpD is involved in cell separation; however, deletion of nlpD did not affect in vitro growth rate. Trans-complementation experiments with the Y. pestis nlpD gene restored virulence and all other phenotypic defects. Finally, we demonstrated that subcutaneous administration of the nlpD mutant could protect animals against bubonic and primary pneumonic plague. Taken together, these results demonstrate that Y. pestis NlpD is a novel virulence factor essential for the development of bubonic and pneumonic plague. Further, the nlpD mutant is superior to the EV76 prototype live vaccine strain in immunogenicity and in conferring effective protective immunity. Thus it could serve as a basis for a very potent live vaccine against bubonic and pneumonic plague