10 research outputs found
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Dysregulation of lipidomic profile and antiviral immunity in response to hyaluronan in patients with severe asthma.
Acoustofluidic Transfer of Inflammatory Cells from Human Sputum Samples
For
sputum analysis, the transfer of inflammatory cells from liquefied
sputum samples to a culture medium or buffer solution is a critical
step because it removes the inflammatory cells from the presence of
residual dithiothreitol (DTT), a reagent that reduces cell viability
and interferes with further sputum analyses. In this work, we report
an acoustofluidic platform for transferring inflammatory cells using
standing surface acoustic waves (SSAW). In particular, we exploit
the acoustic radiation force generated from a SSAW field to actively
transfer inflammatory cells from a solution containing residual DTT
to a buffer solution. The viability and integrity of the inflammatory
cells are maintained during the acoustofluidic-based cell transfer
process. Our acoustofluidic technique removes residual DTT generated
in sputum liquefaction and facilitates immunophenotyping of major
inflammatory cells from sputum samples. It enables cell transfer in
a continuous flow, which aids the development of an automated, integrated
system for on-chip sputum processing and analysis
Acoustofluidic Transfer of Inflammatory Cells from Human Sputum Samples
For
sputum analysis, the transfer of inflammatory cells from liquefied
sputum samples to a culture medium or buffer solution is a critical
step because it removes the inflammatory cells from the presence of
residual dithiothreitol (DTT), a reagent that reduces cell viability
and interferes with further sputum analyses. In this work, we report
an acoustofluidic platform for transferring inflammatory cells using
standing surface acoustic waves (SSAW). In particular, we exploit
the acoustic radiation force generated from a SSAW field to actively
transfer inflammatory cells from a solution containing residual DTT
to a buffer solution. The viability and integrity of the inflammatory
cells are maintained during the acoustofluidic-based cell transfer
process. Our acoustofluidic technique removes residual DTT generated
in sputum liquefaction and facilitates immunophenotyping of major
inflammatory cells from sputum samples. It enables cell transfer in
a continuous flow, which aids the development of an automated, integrated
system for on-chip sputum processing and analysis
Acoustofluidic Transfer of Inflammatory Cells from Human Sputum Samples
For
sputum analysis, the transfer of inflammatory cells from liquefied
sputum samples to a culture medium or buffer solution is a critical
step because it removes the inflammatory cells from the presence of
residual dithiothreitol (DTT), a reagent that reduces cell viability
and interferes with further sputum analyses. In this work, we report
an acoustofluidic platform for transferring inflammatory cells using
standing surface acoustic waves (SSAW). In particular, we exploit
the acoustic radiation force generated from a SSAW field to actively
transfer inflammatory cells from a solution containing residual DTT
to a buffer solution. The viability and integrity of the inflammatory
cells are maintained during the acoustofluidic-based cell transfer
process. Our acoustofluidic technique removes residual DTT generated
in sputum liquefaction and facilitates immunophenotyping of major
inflammatory cells from sputum samples. It enables cell transfer in
a continuous flow, which aids the development of an automated, integrated
system for on-chip sputum processing and analysis