A dissimilarity based on relevant population features

In this paper a dissimilarity index between statistical populations is pro-posed without the hypothesis of a specific statistical model. We assume that the studied populations differ on some relevant features which are measured trough convenient parameters of interest. We assume also that we dispose of adequate estimators for these parameters. To measure the differences be-tween populations with respect the parameters of interest, we construct an index inspired on some properties of the information metric which are also presented. Additionally, we consider several examples and compare the ob-tained dissimilarity index with some other distances, like Mahalanobis or Siegel distances

A graphical classification of European countries according to physical activity Level of its citizens

Data on self-reported frequency of exercising or playing sport of adults aged 15 and above in 27 EU countries were collected, from the European Commission's Special Eurobarometer. A graphical output was obtained using classical a statistical methodology known as metric Multidimensional Scaling method to better define the interrelationships between a large set of variables for the data from the 27 European countries and "average" country included in the study. People in Sweden, Denmark and Finland had the highest level of exercise and playing sport level. High level of exercise and play sport level were detected in Slovenia, the Netherlands, Belgium, Germany, Luxembourg, the United Kingdom and France while low level of exercise and play sport level were found in Romania, Hungary, Italy, Poland and Portugal. The lowest level of exercise and play sport was observed in Bulgaria and Greece. The groups of countries that result from this classification also are characterized by the extent of the difference between the lowest levels of activity (never practising) and the highest (regularly practising); Austria, Czech Republic and Slovakia, have the highest proportion of people who seldom practising. In 4 countries, Ireland, Malta, Republic of Cyprus and Portugal, the proportion of citizens who practice exercise or play sport regularly or never (extreme behaviour) is high. This study shows what a high level and regularly of exercise and playing sport are associated with adults participating in education and training, satisfaction with household financial situation and kind of work activity

Pràctiques d'estadística individualitzades integrant R+exams+Sweave en el banc de preguntes Moodle

Projecte 2014PID‐UB/056En aquest projecte hem utilitzat el paquet “exams” de R per tal d’implementar les pràctiques de les assignatures Estadística de primer curs dels graus de Bioquímica, Biotecnologia, CC. Biomèdiques i Estadística. Les pràctiques d’aquests graus havien utilitzat fins el curs passat un aplicatiu desenvolupat en Java pel propi grup. L’experiència adquirida utilitzant exams en un projecte d’innovació PMID a l'assignatura Disseny d'Experiments de quart curs del grau de CC Biomèdiques ens va animar a substituir la nostre anterior plataforma per la combinació “exams” i Sweave per tal de dissenyar una col·lecció de preguntes individualitzades (una mateixa pregunta pot tenir moltes variants, cadascuna amb diferents dades associades). La col·lecció de preguntes s’importa al banc de preguntes de Moodle i després s’integren en qüestionaris del campus. El campus virtual s’encarrega de subministrar aleatòriament a cada estudiant una d’aquestes variants i de corregir automàticament les respostes. Seguint aquesta tecnologia s’han confeccionat una vintena de qüestionaris equivalents en prestacions i facilitat d’ús a les activitats on-line dels projectes tradicionals Statmedia. Aprofitant el canvi tecnològic s’han revisat els continguts de totes les pràctiques. Els avantatges respecte a la situació anterior són: 1) Sweave és molt conegut entre els usuaris de R i facilita la creació i modificació de les pràctiques per part dels professors “creadors” de material, 2) la gestió d’usuaris, terminis d’activitats i notes queda resolta pel propi campus virtual i simplifica notablement la tasca d’administració dels professors del grup d’innovació Statmedia. Els estudiants han realitzat les pràctiques i complimentat els formularis sense incidències remarcables malgrat el doble canvi de tecnologia i de continguts. El resultat global ha estat molt satisfactori i estem disposats en un proper projecte PMID a dissenyar amb exams totes les activitats on-line dels cursos que imparteixi el grup.Projecte 2014PID‐UB/05

A high-corn-oil diet strongly stimulates mammary carcinogenesis, while a high extra virgin-olive-oil diet has a weak effect, through changes in metabolism, immune system function, and proliferation/apoptosis pathways.

Breast cancer is the most common malignancy in women worldwide, and dietary lipids are important environmental factors influencing its etiology. We have investigated the effects, and the mechanisms associated, of high-fat diets on 7,12-dimethylbenz(a)anthracene-induced rat mammary tumors. Animals were fed a low-fat, a high-corn-oil (HCO) or a high-extra-virgin-olive-oil (HOO) diet from weaning or after induction. The HCO diet had a clear stimulating effect on mammary carcinogenesis, especially when dietary intervention started after induction, whereas the tumors from HOO diet groups exhibited clinical and morphological characteristics similar to those from low-fat controls. Transcriptomic and further protein and immunohistochemical analyses of tumors also indicated different modulatory effects of high-fat diets affecting relevant biological functions: metabolism, immunosurveillance and proliferation/apoptosis pathways. Thus, the results suggested different metabolic adaptations with increased glycolysis by effect of HOO diet. Moreover, leukocyte tumor infiltration and inflammation mediators showed increased cytotoxic T cells and decreased TGFβ1 expression by the HOO diet, while the HCO one increased arginase expression and IL-1α plasma levels. Furthermore, the study of proteins controlling proliferation/apoptosis pathways (Sema3A, Stat5, Smad1, Casp3) suggested an increase in proliferation by the HCO diet and an increase of apoptosis by the diet rich in olive oil. In conclusion, the HCO diet clearly stimulated mammary carcinogenesis, especially in the promotion phase, and induced molecular changes suggesting increased tumor proliferation/apoptosis balance and a proinflammatory microenvironment. The HOO diet, despite being high fat, had a weaker effect on tumorigenesis probably related to metabolic adaptations, enhanced immunosurveillance and increased apoptosis

A high-corn-oil diet strongly stimulates mammary carcinogenesis, while a high-extra-virgin-olive-oil diet has a weak effect, through changes in metabolism, immune system function and proliferation/apoptosis pathways

Breast cancer is the most common malignancy in women worldwide, and dietary lipids are important environmental factors influencing its etiology. We have investigated the effects, and the mechanisms associated, of high-fat diets on 7,12-dimethylbenz(a)anthracene-induced rat mammary tumors. Animals were fed a low-fat, a high-corn-oil (HCO) or a high-extra-virgin-olive-oil (HOO) diet from weaning or after induction. The HCO diet had a clear stimulating effect on mammary carcinogenesis, especially when dietary intervention started after induction, whereas the tumors from HOO diet groups exhibited clinical and morphological characteristics similar to those from low-fat controls. Transcriptomic and further protein and immunohistochemical analyses of tumors also indicated different modulatory effects of high-fat diets affecting relevant biological functions: metabolism, immunosurveillance and proliferation/apoptosis pathways. Thus, the results suggested different metabolic adaptations with increased glycolysis by effect of HOO diet. Moreover, leukocyte tumor infiltration and inflammation mediators showed increased cytotoxic T cells and decreased TGFβ1 expression by the HOO diet, while the HCO one increased arginase expression and IL-1α plasma levels. Furthermore, the study of proteins controlling proliferation/apoptosis pathways (Sema3A, Stat5, Smad1, Casp3) suggested an increase in proliferation by the HCO diet and an increase of apoptosis by the diet rich in olive oil. In conclusion, the HCO diet clearly stimulated mammary carcinogenesis, especially in the promotion phase, and induced molecular changes suggesting increased tumor proliferation/apoptosis balance and a proinflammatory microenvironment. The HOO diet, despite being high fat, had a weaker effect on tumorigenesis probably related to metabolic adaptations, enhanced immunosurveillance and increased apoptosis

Generación automática de cuestionarios Moodle con el paquete exams de R

Se presenta como se puede generar automáticamente cuestionarios Moodle usando el paquete exams de R usando informes dinámicos con latex y R

Circulating EZH2-positive T cells are decreased in multiple sclerosis patients

BACKGROUND: Recent studies in experimental autoimmune encephalomyelitis, an animal model of multiple sclerosis (MS), suggest an involvement of the histone methyltransferase enhancer of zeste 2 polycomb repressive complex 2 subunit (EZH2) in important processes such as cell adhesion and migration. METHODS: Here, we aimed to expand these initial observations by investigating the role of EZH2 in MS. mRNA expression levels for EZH2 were measured by real-time PCR in peripheral blood mononuclear cells (PBMC) from 121 MS patients (62 untreated and 59 receiving treatment) and 24 healthy controls. RESULTS: EZH2 expression levels were decreased in PBMC from untreated patients compared to that from controls, and treatment significantly upregulated EZH2 expression. Expression of miR-124 was increased in MS patients compared to controls. Blood immunophenotyping revealed EZH2 expression mostly restricted to CD4+ and CD8+ T cells, and circulating EZH2+ CD4+ and CD8+ T cells were decreased in untreated MS patients compared to controls. CD8+ T cells expressing EZH2 exhibited a predominant central memory phenotype, whereas EZH2+ CD4+ T cells were of effector memory nature, and both T cell subsets produced TNF-α. EZH2+ T cells were enriched in the cerebrospinal fluid compartment compared to blood and were found in chronic active lesions from MS patients. EZH2 inhibition and microarray analysis in PBMC was associated with significant downregulation of key T cell adhesion molecules. CONCLUSION: These findings suggest a role of EZH2 in the migration of T cells in MS patients. The observation of TNF-α expression by CD4+ and CD8+ T cells expressing EZH2 warrants additional studies to explore more in depth the pathogenic potential of EZH2+-positive cells in MS

Hospital at Home treatment with Remdesivir for patients with COVID-19: Real life experience.

Background : Access and appropriateness of therapeutics for COVID-19 vary due to access or regulatory barriers, severity of disease, and for some therapies, stage of the pandemic and circulating variants. Remdesivir has shown benefits in clinical recovery and is the treatment of choice for selected patients, both hospitalized and non-hospitalized, in main international guidelines. The use of Remdesivir in alternatives to conventional hospitalization such as Hospital at Home units remain incompletely explored. In this work, we aim to describe the real-life experience of outpatient remdesivir infusion for COVID-19 in a Hospital at Home unit. Methods : We selected all the consecutive patients receiving remdesivir from a prospective cohort of 507 COVID-19 patients admitted at a Hospital at Home unit. Admission criteria included COVID-19 with FiO2 requirement under 0.35 and respiratory rate under 22 rpm. Patients were daily assessed in-person by a nurse and a physician. Results : Two-hundred thirty-six patients admitted in HaH received remdesivir, from whom 172 were treated at home. Only 2% presented any adverse event related to the infusion, all of them mild. HaH saved 1416 day-beds, with only 5% of the patients requiring transfer back to hospital. Conclusions : Remdesivir infusion in Hospital at Home units seems to be a safe and efficient alternative to conventional hospitalization for treating non-severe COVID-19 patients

Metabolome biomarkers linking dietary fibre intake with cardiometabolic effects: results from the Danish Diet, Cancer and Health-Next Generations MAX study

Biomarkers associated with dietary fibre intake, as complements to traditional dietary assessment tools, may improve the understanding of its role in human health. Our aim was to discover metabolite biomarkers related to dietary fibre intake and investigate their association with cardiometabolic risk factors. We used data and samples from the Danish Diet Cancer and Health Next Generation (DCH-NG) MAX-study, a one-year observational study with evaluations at baseline, six and 12 months (n = 624, 55% female, mean age: 43 years, 1353 observations). Direct associations between fibre intake and plasma concentrations of 2,6-dihydroxybenzoic acid (2,6-DHBA) and indolepropionic acid were observed at the three time-points. Both metabolites showed an intraclass-correlation coefficient (ICC) > 0.50 and were associated with the self-reported intake of wholegrain cereals, and of fruits and vegetables, respectively. Other metabolites associated with dietary fibre intake were linolenoyl carnitine, 2-aminophenol, 3,4-DHBA, and proline betaine. Based on the metabolites associated with dietary fibre intake we calculated predicted values of fibre intake using a multivariate, machine-learning algorithm. Metabolomics-based predicted fibre, but not self-reported fibre values, showed negative associations with cardiometabolic risk factors (i.e. high sensitivity C-reactive protein, systolic and diastolic blood pressure, all FDR-adjusted p-values <0.05). Furthermore, different correlations with gut microbiota composition were observed. In conclusion, 2,6-DHBA and indolepropionic acid in plasma may better link dietary fibre intake with its metabolic effects than self-reported values. These metabolites may represent a novel class of biomarkers reflecting both dietary exposure and host and/or gut microbiota characteristics providing a read-out that is differentially related to cardiometabolic risk

Medicalized Hotel as an Alternative to Hospital Care for Management of Noncritical COVID-19

Background: Since the first wave of COVID-19, alternatives to conventional hospitalization have been proposed for the provision of different levels of care, ranging from shelter during quarantine to hospital-level medical care. Objective: To describe the adaptation of a hotel by a hospital-at-home team to provide hospital-level care to patients with COVID-19 during the first wave of the pandemic in Barcelona, Spain. Methods: Hospital Clínic de Barcelona (HCB) is a 750-bed, public, tertiary teaching hospital serving 560 000 persons in the metropolitan area of Barcelona, Spain. In March 2020, the hospital-at-home unit was instructed to medicalize a hotel ('health hotel' [HH]) in downtown Barcelona. The aim of this initiative was to help decongest hospitals in the area by admitting patients with low dependency (Barthel Index score >60) and mild to severe COVID-19 from emergency departments or COVID-19 hospital wards, according to Centers for Disease Control and Prevention clinical guideline