Enhancement of homology-directed repair with chromatin donor templates in cells.

A key challenge in precise genome editing is the low efficiency of homology-directed repair (HDR). Here we describe a strategy for increasing the efficiency of HDR in cells by using a chromatin donor template instead of a naked DNA donor template. The use of chromatin, which is the natural form of DNA in the nucleus, increases the frequency of HDR-edited clones as well as homozygous editing. In addition, transfection of chromatin results in negligible cytotoxicity. These findings suggest that a chromatin donor template should be useful for a wide range of HDR applications such as the precise insertion or replacement of DNA fragments that contain the coding regions of genes

Larval description and chaetotaxic analysis of dineutus sinuosipennis laporte, 1840, with a key for the identification of larvae of the tribe Dineutini (Coleoptera, Gyrinidae)

The larvae of the Malagasy whirligig beetle Dineutus sinuosipennis Laporte, 1840, identified using DNA sequence data, are described and illustrated for the first time, including detailed morphometric and chaetotaxic analyses of selected structures and a description of larval habitat. Larvae of the genus Dineutus Macleay, 1825 are diagnosed, and a key to identify the genera of the tribe Dineutini is presented. Larvae of Dineutus exhibit the characters traditionally recognized as autapomorphies of the Gyrinidae: body less sclerotized, egg bursters located on the parietal, one additional sensorial plate on the third antennomere, cardo and lacinia well developed, prementum completely divided, abdominal tracheal gills, and four terminal hooks on the pygopod. They also share with larvae of the other Dineutini genera these putative synapomorphies: numerous minute pore-like additional structures on the ultimate maxillary and labial palpomeres, coxal primary seta CO12 inserted submedially, and trochanteral primary seta TR2 absent. Larvae of Dineutus can be distinguished from those of other known genera of Dineutini by the posterior margin of the lacinia not dentate, tracheal gills plumose, parietal seta PA5 inserted relatively far from setae PA7–9, mandibular pores MNb and MNc inserted relatively far from each other, and tarsal seta TA1 inserted submedially.Fil: Michat, Mariano Cruz. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Biodiversidad y Biología Experimental y Aplicada. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Biodiversidad y Biología Experimental y Aplicada; ArgentinaFil: Gustafson, Grey T.. University of Kansas; Estados UnidosFil: Bergsten, Johannes. Swedish Museum Of Natural History; Sueci

The On-Site and Downstream Costs of Soil Erosion in the Magat and Pantabangan Watersheds

This article report is a condensed version of “The On-Site and Downstream Costs of Soil Erosion,” PIDS Technical Report 87-04 of the Upland Resource Policy Program. It estimates the economic costs arising from indiscriminate exploitation of the uplands. It also offers a practical methodology for assessing the economic impact of soil erosion and illustrates the methodology with results from two major watersheds--Magat and Pantabangan.watershed, uplands, soil erosion, soil conservation

The On-Site and Downstream Costs of Soil Erosion in the Magat and Pantabangan Watersheds

This article report is a condensed version of “The On-Site and Downstream Costs of Soil Erosion,” PIDS Technical Report 87-04 of the Upland Resource Policy Program. It estimates the economic costs arising from indiscriminate exploitation of the uplands. It also offers a practical methodology for assessing the economic impact of soil erosion and illustrates the methodology with results from two major watersheds--Magat and Pantabangan.watershed, uplands, soil erosion, soil conservation

Report on "Scheduling in a factory"

In order to carry out their orders of shoe soles, this company has a number of tasks T_1, ..., T_n of different lengths to be assigned to groups of machines. Each group is operated by one worker (two in one case), and an operation cycle corresponds to injection, cooling, and removal of the sole. The time taken at each step varies from one order to another, and when starting a new task a machine needs to be tuned, which takes some extra time. Machines are working in parallel. At the moment the assignment is carried out empirically, and the problem proposed is to optimize the procedure