The Influence of the Hudson's Bay Company in the Exploration and Settlement of the Red River Valley of the North

As beaver became scarcer in the east, the quest for Castor Canadensis sent traders into the northern plains. Reluctant explorers, traders looked for easier access and cheaper means of transport. Initially content to wait on the shores of the Bay, HBC was forced to meet their competitors in the natives? homelands. The Red River Valley was easily accessed from Hudson?s Bay, becoming the center of the fur trade in the northern plains. HBC helped colonize the first permanent settlement west of the Great Lakes in the Red River Valley. Allowing white women and introducing cultivation into the area was a necessary change. The influence of the fur trade in North Dakota and of the Hudson?s Bay Company on the exploration and settlement of the Red River Valley cannot be overemphasized

Rural Implications of Geographic Rating of Health Insurance Premiums

This brief examines how and to what extent states allow health plans to vary premiums by geographic rating area and, using insurance data from selected states, assesses the direction and magnitude of variations in rural and urban geographic rating factors. The authors conclude with a discussion of strategies that federal and state policymakers might use to help ensure that premium variations based on geography are justified. KEY POINTS: There is no clear pattern of geographic rating factors favoring rural or urban areas. This lack of a clear pattern suggests that health plans may use geographic rating for business purposes other than adjusting for underlying cost/price differences. Geographic rating could reduce insurance risk pooling and be used as a proxy for experience rating. To limit the effect of market segmentation resulting from geographic rating, rate bands could be imposed on area rating factors

Cryo-EM structure of the monomeric Rhodobacter sphaeroides RC-LH1 core complex at 2.5 Å.

Reaction centre light-harvesting 1 (RC-LH1) complexes are the essential components of bacterial photosynthesis. The membrane-intrinsic LH1 complex absorbs light and the energy migrates to an enclosed RC where a succession of electron and proton transfers conserves the energy as a quinol, which is exported to the cytochrome bc1 complex. In some RC-LH1 variants quinols can diffuse through small pores in a fully circular, 16-subunit LH1 ring, while in others missing LH1 subunits create a gap for quinol export. We used cryogenic electron microscopy to obtain a 2.5 Å resolution structure of one such RC-LH1, a monomeric complex from Rhodobacter sphaeroides. The structure shows that the RC is partly enclosed by a 14-subunit LH1 ring in which each αβ heterodimer binds two bacteriochlorophylls and, unusually for currently reported complexes, two carotenoids rather than one. Although the extra carotenoids confer an advantage in terms of photoprotection and light harvesting, they could impede passage of quinones through small, transient pores in the LH1 ring, necessitating a mechanism to create a dedicated quinone channel. The structure shows that two transmembrane proteins play a part in stabilising an open ring structure; one of these components, the PufX polypeptide, is augmented by a hitherto undescribed protein subunit we designate as protein-Y, which lies against the transmembrane regions of the thirteenth and fourteenth LH1α polypeptides. Protein-Y prevents LH1 subunits 11-14 adjacent to the RC QB site from bending inwards towards the RC and, with PufX preventing complete encirclement of the RC, this pair of polypeptides ensures unhindered quinone diffusion