Hydrodynamic Coupling of Two Brownian Spheres to a Planar Surface

We describe direct imaging measurements of the collective and relative diffusion of two colloidal spheres near a flat plate. The bounding surface modifies the spheres' dynamics, even at separations of tens of radii. This behavior is captured by a stokeslet analysis of fluid flow driven by the spheres' and wall's no-slip boundary conditions. In particular, this analysis reveals surprising asymmetry in the normal modes for pair diffusion near a flat surface.Comment: 4 pages, 4 figure

Repeated stimulation of the HPA axis alters white blood cell count without increasing oxidative stress or inflammatory cytokines in fasting elephant seal pups

The hypothalamic-pituitary-adrenal (HPA) axis controls the release of glucocorticoids, which regulate immune and inflammatory function by modulating cytokines, white blood cells and oxidative stress via glucocorticoid receptor (GR) signaling. Although the response to HPA activation is well characterized in many species, little is known about the impacts of HPA activation during extreme physiological conditions. Hence, we challenged 18 simultaneously fasting and developing elephant seal pups with daily intramuscular injections of adrenocorticotropin (ACTH), a GR antagonist (RU486), or a combination of the two (ACTH+RU486) for 4 days. We collected blood at baseline, 2 h and 4 days after the beginning of treatment. ACTH and ACTH+RU486 elevated serum aldosterone and cortisol at 2 h, with effects diminishing at 4 days. RU486 alone induced a compensatory increase in aldosterone, but not cortisol, at 4 days. ACTH decreased neutrophils at 2 h, while decreasing lymphocytes and increasing the neutrophil:lymphocyte ratio at 4 days. These effects were abolished by RU486. Despite alterations in white blood cells, there was no effect of ACTH or RU486 on transforming growth factor-β or interleukin-6 levels; however, both cytokines decreased with the 4 day fasting progression. Similarly, ACTH did not impact protein oxidation, lipid peroxidation or antioxidant enzymes, but plasma isoprostanes and catalase activity decreased while glutathione peroxidase increased with fasting progression. These data demonstrate differential acute (2 h) and chronic (4 days) modulatory effects of HPA activation on white blood cells and that the chronic effect is mediated, at least in part, by GR. These results also underscore elephant seals\u27 extraordinary resistance to oxidative stress derived from repeated HPA activation

Faraday Tomography of the North Polar Spur: Constraints on the distance to the Spur and on the Magnetic Field of the Galaxy

We present radio continuum and polarization images of the North Polar Spur (NPS) from the Global Magneto-Ionic Medium Survey (GMIMS) conducted with the Dominion Radio Astrophysical Observatory 26-m Telescope. We fit polarization angle versus wavelength squared over 2048 frequency channels from 1280 to 1750 MHz to obtain a Faraday Rotation Measure (RM) map of the NPS. Combining this RM map with a published Faraday depth map of the entire Galaxy in this direction, we derive the Faraday depth introduced by the NPS and the Galactic interstellar medium (ISM) in front of and behind the NPS. The Faraday depth contributed by the NPS is close to zero, indicating that the NPS is an emitting only feature. The Faraday depth caused by the ISM in front of the NPS is consistent with zero at b>50 degree, implying that this part of the NPS is local at a distance of approximately several hundred parsecs. The Faraday depth contributed by the ISM behind the NPS gradually increases with Galactic latitude up to b=44 degree, and decreases at higher Galactic latitudes. This implies that either the part of the NPS at b<44 degree is distant or the NPS is local but there is a sign change of the large-scale magnetic field. If the NPS is local, there is then no evidence for a large-scale anti-symmetry pattern in the Faraday depth of the Milky Way. The Faraday depth introduced by the ISM behind the NPS at latitudes b>50 degree can be explained by including a coherent vertical magnetic field.Comment: 9 pages, 8 figures, accepted for publication in ApJ. Some figures have been degraded to reduce sizes, for a high resolution version, see http://physics.usyd.edu.au/~xhsun/ms_nps.pd

Strong Attraction between Charged Spheres due to Metastable Ionized States

We report a mechanism which can lead to long range attractions between like-charged spherical macroions, stemming from the existence of metastable ionized states. We show that the ground state of a single highly charged colloid plus a few excess counterions is overcharged. For the case of two highly charged macroions in their neutralizing divalent counterion solution we demonstrate that, in the regime of strong Coulomb coupling, the counterion clouds are very likely to be unevenly distributed, leading to one overcharged and one undercharged macroion. This long-living metastable configuration in turn leads to a long range Coulomb attraction.Comment: REVTEX-published versio