Dual function of HYPONASTIC LEAVES 1 during early skotomorphogenic growth in Arabidopsis

Seeds germinating underground display a specific developmental programme, termed skotomorphogenesis, to ensure survival of the emerging seedlings until they reach the light. They rapidly elongate the hypocotyl and maintain the cotyledons closed, forming a hook with the hypocotyl in order to protect apical meristematic cells from mechanical damage. Such crucial events for the fate of the seedling are tightly regulated and although some transcriptional regulators and phytohormones are known to be implicated in this regulation, we are still far from a complete understanding of these biological processes. Our work provides information on the diverse roles in skotomorphogenesis of the core components of microRNA biogenesis in Arabidopsis, HYL1, DCL1, and SE. We show that hypocotyl elongation is promoted by all these components, probably through the action of specific miRNAs. Hook development also depends on these proteins however, remarkably, HYL1 exerts its role in an opposite way to DCL1 and SE. Interestingly, we found that a specific HYL1 domain involved in protein–protein interaction is required for this function. Genetic evidences also point to the phosphorylation status of HYL1 as important for this function. We propose that HYL1 help maintain the hook closed during early skotomorphogenesis in a microprocessor-independent manner by repressing the activity of HY5, the transcriptional master regulator that triggers light responses. This work uncovers a previously unnoticed link between components of the miRNA biogenesis machinery, the skotomorphogenic growth, and hook development in Arabidopsis.Fil: Sacnun, Juan Manuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; ArgentinaFil: Crespo, Roberta. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; ArgentinaFil: Palatnik, Javier Fernando. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; ArgentinaFil: Rasia, Rodolfo Maximiliano. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; ArgentinaFil: Gonzalez Schain, Nahuel Damian. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentin

A massive nebula around the Luminous Blue Variable star RMC143 revealed by ALMA

The luminous blue variable (LBV) RMC143 is located in the outskirts of the 30~Doradus complex, a region rich with interstellar material and hot luminous stars. We report the $3\sigma$ sub-millimetre detection of its circumstellar nebula with ALMA. The observed morphology in the sub-millimetre is different than previously observed with HST and ATCA in the optical and centimetre wavelength regimes. The spectral energy distribution (SED) of RMC143 suggests that two emission mechanisms contribute to the sub-mm emission: optically thin bremsstrahlung and dust. Both the extinction map and the SED are consistent with a dusty massive nebula with a dust mass of $0.055\pm0.018~M_{\odot}$ (assuming $\kappa_{850}=1.7\rm\,cm^{2}\,g^{-1}$). To date, RMC143 has the most dusty LBV nebula observed in the Magellanic Clouds. We have also re-examined the LBV classification of RMC143 based on VLT/X-shooter spectra obtained in 2015/16 and a review of the publication record. The radiative transfer code CMFGEN is used to derive its fundamental stellar parameters. We find an effective temperature of $\sim 8500$~K, luminosity of log$(L/L_{\odot}) = 5.32$, and a relatively high mass-loss rate of $1.0 \times 10^{-5}~M_{\odot}$~yr$^{-1}$. The luminosity is much lower than previously thought, which implies that the current stellar mass of $\sim8~M_{\odot}$ is comparable to its nebular mass of $\sim 5.5~M_{\odot}$ (from an assumed gas-to-dust ratio of 100), suggesting that the star has lost a large fraction of its initial mass in past LBV eruptions or binary interactions. While the star may have been hotter in the past, it is currently not hot enough to ionize its circumstellar nebula. We propose that the nebula is ionized externally by the hot stars in the 30~Doradus star-forming region.Comment: Paper accepted by A&A on 09/05/2019 and in proof stage. Second comments by referee are included in this versio

Dust properties inside molecular clouds from coreshine modeling and observations

Context. Using observations to deduce dust properties, grain size distribution, and physical conditions in molecular clouds is a highly degenerate problem. Aims. The coreshine phenomenon, a scattering process at 3.6 and 4.5 $\mu$m that dominates absorption, has revealed its ability to explore the densest parts of clouds. We want to use this effect to constrain the dust parameters. The goal is to investigate to what extent grain growth (at constant dust mass) inside molecular clouds is able to explain the coreshine observations. We aim to find dust models that can explain a sample of Spitzer coreshine data. We also look at the consistency with near-infrared data we obtained for a few clouds. Methods. We selected four regions with a very high occurrence of coreshine cases: Taurus-Perseus, Cepheus, Chameleon and L183/L134. We built a grid of dust models and investigated the key parameters to reproduce the general trend of surface bright- nesses and intensity ratios of both coreshine and near-infrared observations with the help of a 3D Monte-Carlo radiative transfer code. The grid parameters allow to investigate the effect of coagulation upon spherical grains up to 5 $\mu$m in size derived from the DustEm diffuse interstellar medium grains. Fluffiness (porosity or fractal degree), ices, and a handful of classical grain size distributions were also tested. We used the near- and mostly mid-infrared intensity ratios as strong discriminants between dust models. Results. The determination of the background field intensity at each wavelength is a key issue. In particular, an especially strong background field explains why we do not see coreshine in the Galactic plane at 3.6 and 4.5 $\mu$m. For starless cores, where detected, the observed 4.5 $\mu$m / 3.6 $\mu$m coreshine intensity ratio is always lower than $\sim$0.5 which is also what we find in the models for the Taurus-Perseus and L183 directions. Embedded sources can lead to higher fluxes (up to four times greater than the strongest starless core fluxes) and higher coreshine ratios (from 0.5 to 1.1 in our selected sample). Normal interstellar radiation field conditions are sufficient to find suitable grain models at all wavelengths for starless cores. The standard interstellar grains are not able to reproduce observations and, due to the multi-wavelength approach, only a few grain types meet the criteria set by the data. Porosity does not affect the flux ratios while the fractal dimension helps to explain coreshine ratios but does not seem able to reproduce near-infrared observations without a mix of other grain types. Conclusions. Combined near- and mid-infrared wavelengths confirm the potential to reveal the nature and size distribution of dust grains. Careful assessment of the environmental parameters (interstellar and background fields, embedded or nearby reddened sources) is required to validate this new diagnostic

Anomalous Microwave Emission in HII Regions: Is it Really Anomalous? The Case of RCW 49

The detection of an excess of emission at microwave frequencies with respect to the predicted free–free emission has been reported for several Galactic H ii regions. Here, we investigate the case of RCW 49, for which the Cosmic Background Imager tentatively (~3σ) detected Anomalous Microwave Emission (AME) at 31 GHz on angular scales of 7'. Using the Australia Telescope Compact Array, we carried out a multi-frequency (5, 19, and 34 GHz) continuum study of the region, complemented by observations of the H109α radio recombination line. The analysis shows that: (1) the spatial correlation between the microwave and IR emission persists on angular scales from 3farcm4 to 0farcs4, although the degree of the correlation slightly decreases at higher frequencies and on smaller angular scales; (2) the spectral indices between 1.4 and 5 GHz are globally in agreement with optically thin free–free emission, however, ~30% of these are positive and much greater than −0.1, consistent with a stellar wind scenario; and (3) no major evidence for inverted free–free radiation is found, indicating that this is likely not the cause of the Anomalous Emission in RCW 49. Although our results cannot rule out the spinning dust hypothesis to explain the tentative detection of AME in RCW 49, they emphasize the complexity of astronomical sources that are very well known and studied, such as H ii regions, and suggest that, at least in these objects, the reported excess of emission might be ascribed to alternative mechanisms such as stellar winds and shocks

Briefing: improving children and young people's mental health services: local data insights from England, Scotland and Wales.

In this briefing, we present analysis from the Networked Data Lab (NDL). Led by the Health Foundation, the NDL is a collaborative network of local analytical teams across England, Scotland and Wales. These teams analysed local, linked data sources to explore trends in mental health presentations across primary, specialist and acute services. This briefing includes: a) background on the trends in mental health disorders among children and young people and existing pressures on services, as well as an overview of the main policies in place in England, Scotland, and Wales to improve children and young people's mental health b) findings from NDL partners: we examine trends and patterns of service use, including the use of primary care, specialist mental health care and acute services, along with differences by demographic and socioeconomic characteristics c) examples of how local NDL teams used linked data to improve services in their area d) insights for national and local policymakers

GESTIÓN DE CONOCIMIENTOS PARA UN DESARROLLO SOSTENIBLE: CONTEXTUALIZACIÓN EN LAS CIENCIAS ALIMENTARIAS (Original)

The current investigation started from a diagnostic to identify the knowledge management insufficiencies for a development related to the practical activities, of first year Food Sciences sustainable students. Its objective is aimed at elaborating a methodology that allows the knowledge management for a sustainable development from the Biology subject during the practical activities. The hermeneutical method was applied from the holistic interpretation that allowed developing the knowledge management for a sustainable development during the biological practical activity. The methodology presents as the main practical result, the transformation in knowledge management for the sustainable development, the traditional searching of knowledge from the Biology subject. The practical results of the proposal were valued from the students´ performance in various evaluations applied and through the methodological triangulation that allowed its effectiveness.La investigación partió de un diagnóstico para identificar las insuficiencias en la gestión de conocimientos para el desarrollo sostenible vinculados con las actividades prácticas, en los estudiantes de 1er año, en la carrera de Licenciatura en Ciencias Alimentarias. En la misma se plantea como objetivo elaborar una metodología que permita la gestión del conocimiento para el desarrollo sostenible desde la asignatura Biología durante la actividad práctica. Se aplicó el método hermenéutico desde su interpretación holística que permitió desarrollar la gestión del conocimiento para el desarrollo sostenible durante la actividad práctica biológica. La metodología exhibe como resultado práctico principal, la transformación en gestión del conocimiento para el desarrollo sostenible, la búsqueda tradicional del conocimiento desde la Biología. Los resultados prácticos de la propuesta fueron valorados a partir del desempeño de los estudiantes en las diversas evaluaciones realizadas y mediante una triangulación metodológica aplicada que permitió avalar la efectividad de la misma

Spitzer Characterization of Dust in the Ionized Medium of the Large Magellanic Cloud

A systematic investigation of dust emission associated with the ionized gas has so far been performed only in our Galaxy and for wavelengths longer than 60 {\mu}m. Newly available Spitzer data now offer the opportunity to carry out a similar analysis in the Large Magellanic Cloud (LMC). By cross-correlating Spitzer SAGE (Surveying the Agents of a Galaxy's Evolution) data with the ATCA/Parkes HI 21-cm data, the NANTEN 12CO (J=1-0) data, and both the SHASSA H{\alpha} and the Parkes 6-cm data, we investigate the physical properties of dust associated with the different phases of the gas (atomic, molecular and ionized). In particular, we study the presence and nature of dust from 3.6 to 160 {\mu}m and for various regimes of the ionized gas, spanning emission measures (EM) from \sim 1 pc cm-6 (diffuse component) to \sim 10^3 pc cm-6 (HII regions). Using a dust emission model, and testing our results with several radiation field spectra, we show that dust in the ionized gas is warmer than dust associated with other phases (atomic and molecular). We also find a decrease of the polycyclic aromatic hydrocarbons (PAH) relative abundance with respect to big grains (BGs), as well as an increase of the near infrared (NIR) continuum. These three results (e.g. warmer temperature, decrease of PAH abundance and increase of the NIR continuum) are found consistently for all regimes of the ionized gas. On the contrary, the molecular phase appears to provide favorable conditions for the survival of PAHs. Furthermore, the very small grain (VSG) relative abundance tends to increase in the ionized phase, especially in bright HII regions. Last but not least, our analysis shows that the emissivity of dust associated with the ionized gas is lower in the LMC than in our Galaxy, and that this difference is not accounted for by the lower metallicity of the LMC.Comment: Accepted for publication in ApJ, 15 pages, 5 figures, 3 table

A INSERÇÃO DO RESIDENTE EM ENFERMAGEM EM UMA UNIDADE DE INTERNAÇÃO CIRÚRGICA: PRÁTICAS E DESAFIOS

The inclusion of nursing residents can promote changes in the care environment in the hospital ambit. This research aimed to understand whether changes took place or not in the nursing practices, as a result of the insertion of residents of this category in an inpatient unit in a University Hospital, in the perception of the nursing team. This research is exploratory-descriptive, has a qualitative approach, and uses the Bardin analysis technique, from which two categories resulted: The perspective of the nurse technicians and The perspective of the nurses. The participants did not perceive substantive changes in the practices resulting from the insertion of the nursing residents. Intermediate situations were identified, which were understood as challenges to the Program. The improvement of modes of insertion of the resident in the practice settings could contribute to education in nursing, to the process of continuing education in health, and to the care given in the institutions to society.A inclusão de residentes em enfermagem pode promover mudanças no ambiente do cuidado em âmbito hospitalar. Esta pesquisa objetivou compreender se houve, ou não, mudança nas práticas de enfermagem, a partir da inserção de residentes dessa categoria em uma unidade de internação de Hospital Universitário, na percepção da equipe de enfermagem. Trata-se de pesquisa exploratório-descritiva, de abordagem qualitativa, utilizando a técnica de análise de Bardin e da qual resultaram duas categorias: Perspectiva do profissional técnico de enfermagem e Perspectiva das enfermeiras. Os participantes perceberam não haver mudanças substantivas nas práticas a partir da inserção dos residentes de enfermagem. Foram identificadas situações intermediárias, entendidas como desafios ao Programa. O aperfeiçoamento dos modos de inserção do residente nos cenários de prática poderá contribuir para a educação em enfermagem, o processo de educação permanente em saúde, e para o cuidado prestado nas instituições à sociedade.La inclusión de residentes en enfermería puede promover cambios en el ambiente del cuidado en ámbito hospitalar. Esta investigación tuvo el objetivo de comprender se hubo o no cambio en las prácticas de enfermería observando la inserción de residentes de esa categoría en una unidad de internación de Hospital Universitario, en la percepción del equipo de enfermería. Es una investigación exploratorio descriptiva, de abordaje cualitativo, utilizando la técnica de análisis de Bardin y de la cual resultaron dos categorías: Perspectiva del profesional técnico de enfermería y Perspectiva de las enfermeras. Los participantes percibieron no haber cambios sustantivos en las prácticas a partir de la inserción de los residentes de enfermería. Fueron identificadas situaciones intermediarias, entendidas como desafíos al Programa. El perfeccionamiento de los modos de inserción del residente en los escenarios de práctica podrá contribuir para la educación en enfermería, el proceso de educación permanente en salud, y para el cuidado prestado en las instituciones a la sociedad