δ Orionis: Further temporal variability and evidence for small-scale structure in the interstellar medium

We report here the detection of both spatial and temporal variations in interstellar absorption in the line of sight to δ Orionis. First, we present new high-resolution (R≈110 000) observations of the interstellar D lines of Na i towards both δ Ori A and C. Comparison of these spectra highlights variations in absorption between the two stars, indicative of small-scale spatial structure in the interstellar medium in this direction over distances of less than ≈15 000 au (the projected separation of the two stars). Components with the largest Na i column densities and lowest velocity dispersions are, in general, found to be subject to the greatest differences; in fact the narrowest component detected is only observed in one of the sightlines. This effect has also been reported by Meyer & Blades. Secondly, we present new ultra-high-resolution (R≈900 000) Na i D1 observations and high-resolution (R≈110 000) Ca ii H & K observations of δ Ori A which, through ultra-high-resolution work conducted between 1994 and 2000, has been shown to exhibit a time-variable interstellar Na i absorption component. These new observations, while revealing the further reduction in intensity of the time-variable Na i absorption, indicate constant Ca ii absorption over the same period. This results in a dramatic reduction in the Na°/Ca+ abundance ratio, perhaps indicating the line of sight to be gradually probing a less-dense outer region of an absorbing filament

Detection of a variable interstellar absorption component towards δ Orionis A

Observations of δ Ori A made with the UHRF in its highest resolution mode (R≈900 000) have revealed the presence of a cool (Tk⩽350 K) variable absorption component at a heliocentric velocity of +21.3 km s−1. The component is detected in Na I D1, where clear hyperfine splitting is seen, and Ca II K. Comparison of our data with existing spectra suggests that the component has consistently increased in strength from 1966 to 1994, and subsequently reduced in intensity by 1999. Following a discussion of the possible origins of this component it is concluded that an interstellar, rather than circumstellar, origin is most likely. This is one of very few detections of variable interstellar absorption reported in the literature, and we suggest an origin within filamentary material associated with the expanding H I shell surrounding the Orion-Eridanus superbubble

A comprehensive framework for assessing the life-cycle energy of building construction assemblies

Building environmental design typically focuses on improvements to operational efficiencies such as building thermal performance and system efficiency. Often the impacts occurring across the other stages of a building\u27s life are not considered or are seen as insignificant in comparison. However, previous research shows that embodied impacts can be just as important. There is limited consistent and comprehensive information available for building designers to make informed decisions in this area. Often the information that is available is from disparate sources, which makes comparison of alternative solutions unreliable. It is also important to ensure that strategies to reduce environmental impacts from one life cycle stage do not come at the expense of an increase in overall life-cycle impacts. A consistent and comprehensive framework for assessing and specifying building assemblies for enhanced environmental outcomes does not currently exist. This article presents the initial findings of a project that aims to establish a database of life cycle energy requirements for a broad range of construction assemblies, based on a comprehensive assessment framework. Life cycle energy requirements have been calculated for eight residential construction assemblies integrating an innovative embodied energy assessment technique with thermal performance modelling and ranked according to their performance. © #2010 Earthscan ISSN: 0003-8628

An ultra-high-resolution study of the interstellar medium towards Orion

We report ultra-high-resolution observations graphic of Na I, Ca II, K I, CH and CH+ for interstellar sightlines towards 12 bright stars in Orion. These data enable the detection of many more absorption components than previously recognized, providing a more accurate perspective on the absorbing medium. This is especially so for the line of sight to the Orion nebula, a region not previously studied at very high resolution. Model fits have been constructed for the absorption-line profiles, providing estimates for the column density, velocity dispersion and central velocity for each constituent velocity component. A comparison between the absorption occurring in sightlines with small angular separations has been used, along with comparisons with other studies, to estimate the line-of-sight velocity structures. Comparisons with earlier studies have also revealed temporal variability in the absorption-line profile of ζ Ori, highlighting the presence of small-scale spatial structure in the interstellar medium on scales of ≈10 au. Where absorption from both Na0 and K0 is observed for a particular cloud, a comparison of the velocity dispersions measured for each of these species provides rigorous limits on both the kinetic temperature and turbulent velocity prevailing in each cloud. Our results indicate the turbulent motions to be subsonic in each case. graphic abundance ratios are derived for individual clouds, providing an indication of their physical state

Pre-Existing Mature Oligodendrocytes Do Not Contribute to Remyelination following Toxin-Induced Spinal Cord Demyelination.

Remyelination is the regenerative response to demyelination. Although the oligodendrocyte progenitor is established as the major source of remyelinating cells, there is no conclusive evidence on whether mature, differentiated oligodendrocytes can also contribute to remyelination. Using two different inducible myelin-CreER mouse strains in which mature oligodendrocytes were prelabeled by the expression of membrane-bound Green fluorescent protein, we found that after focal spinal cord demyelination, the surrounding surviving labeled oligodendrocytes did not proliferate but remained at a consistent density. Furthermore, existing (prelabeled) oligodendrocytes showed no evidence of incorporation or migration into the lesioned area, or of process extension from the peripheral margins into the lesion. Thus, mature oligodendrocytes do not normally contribute to remyelination and are therefore not a promising target for regenerative therapy.Supported by European Research Council grant agreement 293544 (W.D.R.), Wellcome Trust grant WT100269AIA, Medical Research Council grant G0800575, a Royal Society-USA/Canada Exchange Fellowship (I.M.), the UK Multiple Sclerosis Society, and a Wellcome Trust Integrated Veterinary Training Fellowship (A.H.C.)

Survivin as a therapeutic target in Sonic hedgehog-driven medulloblastoma.

Medulloblastoma (MB) is a highly malignant brain tumor that occurs primarily in children. Although surgery, radiation and high-dose chemotherapy have led to increased survival, many MB patients still die from their disease, and patients who survive suffer severe long-term side effects as a consequence of treatment. Thus, more effective and less toxic therapies for MB are critically important. Development of such therapies depends in part on identification of genes that are necessary for growth and survival of tumor cells. Survivin is an inhibitor of apoptosis protein that regulates cell cycle progression and resistance to apoptosis, is frequently expressed in human MB and when expressed at high levels predicts poor clinical outcome. Therefore, we hypothesized that Survivin may have a critical role in growth and survival of MB cells and that targeting it may enhance MB therapy. Here we show that Survivin is overexpressed in tumors from patched (Ptch) mutant mice, a model of Sonic hedgehog (SHH)-driven MB. Genetic deletion of survivin in Ptch mutant tumor cells significantly inhibits proliferation and causes cell cycle arrest. Treatment with small-molecule antagonists of Survivin impairs proliferation and survival of both murine and human MB cells. Finally, Survivin antagonists impede growth of MB cells in vivo. These studies highlight the importance of Survivin in SHH-driven MB, and suggest that it may represent a novel therapeutic target in patients with this disease

Diel turbidity cycles in a headwater stream: evidence of nocturnal bioturbation?

Purpose: A small number of recent studies have linked daily cycles in stream turbidity to nocturnal bioturbation by aquatic fauna, principally crayfish, and demonstrated this process can significantly impact upon water quality under baseflow conditions. Adding to this limited body of research, we use high-resolution water quality monitoring data to investigate evidence of diel turbidity cycles in a lowland, headwater stream with a known signal crayfish (Pacifastacus leniusculus) population and explore a range of potential causal mechanisms. Materials and methods: Automatic bankside monitoring stations measured turbidity and other water quality parameters at 30-min resolution at three locations on the River Blackwater, Norfolk, UK during 2013. Specifically, we focused on two 20-day periods of baseflow conditions during January and April 2013 which displayed turbidity trends typical of winter and spring seasons, respectively. The turbidity time-series, which were smoothed with 6.5 hour Savitzky-Golay filters to highlight diel trends, were correlated against temperature, stage, dissolved oxygen and pH to assess the importance of abiotic influences on turbidity. Turbidity was also calibrated against suspended particulate matter (SPM) over a wide range of values via linear regression. Results and discussion: Pronounced diel turbidity cycles were found at two of the three sites under baseflow conditions during April. Spring night-time turbidity values consistently peaked between 21:00 and 04:00 with values increasing by ~10 nephelometric turbidity units (NTU) compared with the lowest recorded daytime values which occurred between 10:00 and 14:00. This translated into statistically significant increases in median midnight SPM concentration of up to 76% compared with midday, with night-time (18:00 – 05:30) SPM loads also up to 30% higher than that recorded during the daytime (06:00 – 17:30). Relating turbidity to other water quality parameters exhibiting diel cycles revealed there to be neither any correlation that might indicate a causal link, nor any obvious mechanistic connections to explain the temporal turbidity trends. Diel turbidity cycles were less prominent at all sites during the winter. Conclusions: Considering the seasonality and timing of elevated turbidity, visual observations of crayfish activity, and an absence of mechanistic connections with other water quality parameters, the results presented here are consistent with the hypothesis that nocturnal bioturbation is responsible for generating diel turbidity cycles under baseflow conditions in headwater streams. However, further research in a variety of fluvial environments is required to better assess the spatial extent, importance and causal mechanisms of this phenomenon

Fragile X syndrome: Diagnostic and carrier testing

The following are the recommendations of the American College of Medical Genetics (ACMG) Professional Practice and Guidelines Committee, convened to assist health care professionals in making decisions regarding genetic diagnosis and testing. The purpose of this document is to provide a brief overview of fragile X syndrome (FXS), and to make recommendations that can serve as general guidelines to aid clinicians in making referrals for diagnostic and carrier testing for this condition. Fragile X syndrome is the most common cause of inherited mental retardation and is caused by a mutation in the X-linked FMR1 gene. DNA studies are used for testing individuals with symptoms of FXS and individuals at risk for carrying the mutation. Genotypes are determined by examining the size of the trinucleotide repeat segment and the methylation status of the FMR1 gene. These guidelines supersede the 1994 ACMG statement of the same name