Neuronal Cell Model and Methods of Use Thereof

We previously described a novel in vitro model of a non-productive herpes simplex virus type 1 (HSV-1) infection in neurally-differentiated (ND)-PC12 cells that allows for inducible virus replication upon forskolin and heat stress (HS) treatment. In this research, we further characterized the model with respect to HSV-2 strain 333. We found that: (i) ND-PC12 cells are non-permissive to HSV-2 replication; (ii) HSV-2 can establish a quiescent infection, like HSV-1, in ND-PC12 cells with the transient use of acycloguanosine (ACV); however unlike HSV-1, anti-viral conditions are not obligatory to establish and maintain a quiescent state; (iii) the quiescent state is maintained in the presence of Vero cell cocultivation indicating that such cultures are free of infectious virus; and (iv) a high percentage of quiescently infected (QIF)-PC 12 cell cultures (80-100%) produce HSV-2 in response to forskolin and HS (43° C., 3 h) treatment for as long as 4 weeks post infection. These findings indicate that ND-PC12 cells can harbor HSV-2 in a cryptic and non-productive state that is reversible. This model has appealing features for studying gene expression during the establishment, maintenance and reactivation phases of the HSV-2 quiescent state in cell culture. Journal of Neuro Virology (2000) 6, 296-302

Neuronal Cell Model and Methods of Use Therefor

The present invention provides, inter alia, a PC12 cell quiescently infected with a neurotropic virus. Preferred quiescently-infected neural cells are those wherein the neurotropic virus is a neurotropic herpes virus. Methods related to these and other materials are also provided

Interagency Science and Research: Final Project Report

The major accomplishment of this project was the development of the SNAP Science and Research Strategy: an 11-chapter document designed to integrate and coordinate scientific research programs in Southern Nevada and improve the efficiency and effectiveness of these programs. The Strategy is intended to inform and guide SNAP agencies in identifying and articulating highest priority science and research needs, sharing resources and funds from other sources, and eliminating redundancy between research programs within their focus areas. The major focus areas of the Strategy are Fire, Invasive Species, Watersheds and Landscapes, Biodiversity, Cultural Resources, Historic Content, Recreation, Land Use, and Education. The Strategy was approved by the SNAP Board in March 2009

Analysis of multistory frames with light gauge steel panel infills

PREFACE This report was originally presented as a thesis to the Faculty of the Graduate School of Cornell University in partial fulfillment of the requirements for the degree of Doctor of Philosophy, conferred in August 1972. The author wishes to thank Professor Arthur H. Nilson, Project Director, and Professor Robert G. Sexsmith, Principal Investigator, for the help and guidance that made this work possible. This investigation was supported by the American Iron and Steel Institute

The Importance of Background in the Detection and Identification of Gas Plumes Using Emissive Infrared Hyperspectral Sensing

Using a Fourier transform infrared field spectrometer, spectral infrared radiance measurements were made of several generated gas plumes against both a uniform sky and terrestrial background. Background temperature, spectral complexity, and physical homogeneity each influenced the success of emissive infrared spectral sensing technology in detecting and identifying the presence of a gas plume and its component constituents. As expected, high temperature contrast and uniform backgrounds provided the best conditions for detectability and diagnostic identification. This report will summarize some of SITAC’s findings concerning plume detectability, including the importance of plume cooling, plumes in emission and absorption, the effects of optical thickness, and the effects of condensing plumes on gas detection

Quantum wires from coupled InAs/GaAs strained quantum dots

The electronic structure of an infinite 1D array of vertically coupled InAs/GaAs strained quantum dots is calculated using an eight-band strain-dependent k-dot-p Hamiltonian. The coupled dots form a unique quantum wire structure in which the miniband widths and effective masses are controlled by the distance between the islands, d. The miniband structure is calculated as a function of d, and it is shown that for d>4 nm the miniband is narrower than the optical phonon energy, while the gap between the first and second minibands is greater than the optical phonon energy. This leads to decreased optical phonon scattering, providing improved quantum wire behavior at high temperatures. These miniband properties are also ideal for Bloch oscillation.Comment: 5 pages revtex, epsf, 8 postscript figure

January 1 - December 31, 2012

This report summarizes training, education, and outreach activities for calendar 2012 of PTI and affiliated organizations, including the School of Informatics and Computing, Office of the Vice President for Information Technology, and Maurer School of Law. Reported activities include those led by PTI Research Centers (Center for Applied Cybersecurity Research, Center for Research in Extreme Scale Technologies, Data to Insight Center, Digital Science Center) and Service and Cyberinfrastructure Centers (Research Technologies Division of University Information Technology Services, National Center for Genome Assembly Support

Feeding selectivity and rapid particle processing by deep-sea megafaunal deposit feeders: A 234Th tracer approach

Deposit-feeding megafauna occur in virtually all deep-sea environments, yet their feeding selectivity and particle processing rates are poorly known. Excess 234Th activity is commonly used asa geochemical tracer for recently settled (, 100-d old) particles in the quiescent deep sea, but it has rarely been applied to the study of deposit feeders. To explore the selectivity and rates of megafaunal deposit feeding, we compared excess 234Th activities in the gut contents of deposit feeders from Santa Catalina Basin (SCB) (~ 1200 m depth) and the Hawaiian slope (~ 1680 m) to the activity of surface sediments and, in SCB, to material from sediment traps moored, 150 m above the seafloor. We also measured concentrations of chlorophylla and phaeopigments in animal guts and surface sediments to evaluate feeding selectivity. In the SCB, excess 234Th (234Thxs) activities in the guts of four species of surface-deposit feeders were 14–17 fold greater than those of the top 5 mm of sediment. Pannychiamoseleyi and Scotoplanes globosa, two highly mobile, surface-deposit-feeding elasipodid holothuri-ans, were the most enriched in gut 234Thxs activity, suggesting that these species fed very selectivelyon particles settled to the seafloor within the previous, 20 d. Pannychia moseleyi guts also exhibited 500-fold enrichment of chlorophyll a relative to surface sediments indicating highly selective ingestion of phytodetritus. Chiridota sp., a burrowing, surface-deposit-feeding, chiridotid holothurian, and Bathybembix bairdii, a surface-deposit-feeding trochid gastropod, were less enriched in gut 234Thxs activity, reflecting lower mobility and/or less selectivity at time of particle pickup. A subsurface-deposit-feeding, molpadiid holothurian was not enriched in gut 234Thxs activity compared to surface sediments, but was greatly enriched compared to average activities at its presumed feeding depth of 6–7 cm. On the Hawaiian slope, gut contents of two surface-deposit feeders, the synallactidholothurians Mesothuria carnosa and Paleopatides retifer, were not enriched in 234Thxs activity;however, M. carnosa and Phryssocystis sp. (a surface-deposit-feeding echinoid) were enriched in chlorophyll a, suggesting that the Hawaiian slope species are also selective feeders. Presumably, frequent sediment resuspension makes 234Thxs activity a poor tracer for recently settled, food-rich particles on the Hawaiian slope. Based on a newly developed 234Th-flux model, we calculate that the three dominant megafaunal, surface-deposit feeders in SCB consumed on average 39–52% (s.e.13–27%) of the daily flux of 234Thxs activity to the SCB floor. By chemically altering (e.g., digesting) and redistributing recently settled particulate organic matter, these megafauna are likely to substantially influence carbon diagenesis and food-web structure in this bathyal habitat