929 research outputs found

    Agricultural development on the Wet Coast of North Queensland, 1880-1970

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    Audio Fingerprinting to Identify Multiple Videos of an Event

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    The proliferation of consumer recording devices and video sharing websites makes the possibility of having access to multiple recordings of the same occurrence increasingly likely. These co-synchronous recordings can be identified via their audio tracks, despite local noise and channel variations. We explore a robust fingerprinting strategy to do this. Matching pursuit is used to obtain a sparse set of the most prominent elements in a video soundtrack. Pairs of these elements are hashed and stored, to be efficiently compared with one another. This fingerprinting is tested on a corpus of over 700 YouTube videos related to the 2009 U.S. presidential inauguration. Reliable matching of identical events in different recordings is demonstrated, even under difficult conditions

    Detection of Leishmania infantum by PCR, serology and cellular immune response in a cohort study of Brazilian dogs

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    The sensitivity and specificity of PCR, serology (ELISA) and lymphoproliferative response to Leishmania antigen for the detection of Leishmania infantum infection were evaluated in a cohort of 126 dogs exposed to natural infection in Brazil. For PCR, Leishmania DNA from bone-marrow was amplified with both minicircle and ribosomal primers. The infection status and time of infection of each dog were estimated from longitudinal data. The sensitivity of PCR in parasite-positive samples was 98%. However, the overall sensitivity of PCR in post-infection samples, from dogs with confirmed infection, was only 68%. The sensitivity of PCR varied during the course of infection, being highest (78–88%) 0–135 days post-infection and declining to around 50% after 300 days. The sensitivity of PCR also varied between dogs, and was highest in sick dogs. The sensitivity of serology was similar in parasite-positive (84%), PCR-positive (86%) and post-infection (88%) samples. The sensitivity of serology varied during the course of infection, being lowest at the time of infection and high (93–100%) thereafter. Problems in determining the specificity of serology are discussed. The sensitivity and specificity of cellular responsiveness were low. These data suggest that PCR is most useful in detecting active or symptomatic infection, and that serology can be a more sensitive technique for the detection of all infected dogs

    Environmental monitoring of Mycobacterium bovis in badger feces and badger sett soil by real-time PCR, as confirmed by immunofluorescence, immunocapture, and cultivation

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    Real-time PCR was used to detect and quantify Mycobacterium bovis cells in naturally infected soil and badger faeces. Immunomagnetic capture, immunofluorescence and selective culture confirmed species identification and cell viability. These techniques will prove useful for monitoring M. bovis in the environment and for elucidating transmission routes between wildlife and cattle

    Performance of an environmental test to detect Mycobacterium bovis infection in badger social groups

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    A study by Courtenay and others (2006) demonstrated that the probability of detecting Mycobacterium bovis by PCR in soil samples from the spoil heaps of main badger setts correlated with the prevalence of excretion (infectiousness) of captured badgers belonging to the social group. It has been proposed that such a test could be used to target badger culling to setts containing infectious animals (Anon 2007). This short communication discusses the issues surrounding this concept, with the intention of dispelling any misconceptions among relevant stakeholders (farmers, policy makers and conservationists)
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