EXPRESSION IN ESCHERICHIA-COLI OF A DOMINANT IMMUNOGEN OF TRYPANOSOMA-CRUZI RECOGNIZED BY HUMAN CHAGASIC SERA

28351952

The effect of basic fibroblast growth factor on regeneration in a surgical wound model of rat submandibular glands

This study developed an animal model of surgically wounded submandibular glands (SMGs) and investigated the effects of collagen gel with basic fibroblast growth factor (bFGF) on tissue regeneration of surgically wounded SMGs in vivo. The animal model was produced by creating a surgical wound using a 3-mm diameter biopsy punch in SMGs. The wound was filled with collagen gel with bFGF (bFGF group) or without bFGF (control group). In the animal model of surgically wounded SMGs, salivary glands without scar tissue around the wound area were observed with smaller areas of collagen gel. Small round and spindle-shape cells invaded the collagen gel in both groups after operation day (AOD) 5, and this invasion dramatically increased at AOD 7. Host tissue completely replaced the collagen gel at AOD 21. The invading immune cells in the group treated with collagen gel with bFGF were positive for vimentin, α-smooth muscle actin (αSMA), CD49f, c-kit and AQP5 at AOD 7. Similarly, the mRNA expression of vimentin, αSMA, CD49f, keratin19 and AQP5 was also increased. This study suggests that the use of collagen gels with bFGF improves salivary gland regeneration

TRYPANOSOMA-CRUZI - CLONING AND EXPRESSION OF AN ANTIGEN RECOGNIZED BY ACUTE AND CHRONIC HUMAN CHAGASIC SERA

71328429

Álcool, drogas e crime Alcohol, drugs and crime

OBJETIVO: Este artigo descreve duas situações complexas e duplamente preocupantes em termos de saúde pública, seja, por sua alta prevalência e/ou por suas conseqüências. Trata-se dos transtornos do uso de substâncias psicoativas e a criminalidade. Será relatada a forma como diversos pesquisadores as associam, bem como a situação das perícias de usuários e dependentes de álcool e drogas. MÉTODO: Realizou-se uma revisão das publicações sobre o tema, utilizando-se, como bancos de dados, o Medline e o Lilacs, cobrindo o período de 1986 a 2006. Os descritores usados foram: "alcoholism", "drug dependence", "drug abuse" e "crime". Resumos de congressos, artigos e livros relevantes sobre o tema, publicados por diferentes autoridades no assunto, em diversas fases de pesquisa, foram consultados e incluídos. CONCLUSÃO: As diversas pesquisas coincidem na afirmação de uma associação entre transtornos do uso de substâncias psicoativas e criminalidade. O que é possível constatar é a alta proporção de atos violentos quando o álcool ou as drogas ilícitas estão presentes entre agressores, suas vítimas ou em ambos. Quando se realiza um exame pericial em autores que alegam alguma relação do ato praticado com consumo de álcool/drogas, esta perícia deve levar em consideração a substância em uso, o quadro clínico por ela causado, bem como verificar a presença de um diagnóstico, a existência de nexo causal e possíveis alterações na capacidade de entendimento e/ou determinação do agente.<br>OBJECTIVE: This article describes two complex and doubly preoccupying situations in terms of public health, either, for its high prevalence and/or its consequences. These problems are the psychoactive substance use disorder and crime. The form will be told as diverse researchers associate them, as well as the situation of the exam of users and alcohol dependents and drugs. METHOD: A revision of publications was become fulfilled on the subject, using Medline and Lilacs as data bases, covering the period of 1986 the 2006. The keywords used had been: "Alcoholism", "drug dependence", "drug abuses" and "crime". Summaries of congresses, articles and excellent books on the subject, published for different authorities in the subject, in diverse phases of research, had been consulted and enclosed. CONCLUSION: The diverse research coincide in the affirmation of an association between psychoactive substances use disorders and crime. What it is possible to evidence is the high ratio of violent acts when the alcohol or the illicit drugs is used by aggressors, its victims or in both. When it carries through an expert examination in authors who allege some relation of the practiced act with alcohol consumption/drugs, this exam must take in consideration the substance in use, the clinical symptom for caused it, as well as verifying the presence of a diagnosis, the existence of causal nexus and possible alterations in the understanding capacity and/or determination of the agent

Lack of evidence for the pathogenic role of iron and HFE gene mutations in Brazilian patients with nonalcoholic steatohepatitis

The hypothesis of the role of iron overload associated with HFE gene mutations in the pathogenesis of nonalcoholic steatohepatitis (NASH) has been raised in recent years. In the present study, biochemical and histopathological evidence of iron overload and HFE mutations was investigated in NASH patients. Thirty-two NASH patients, 19 females (59%), average 49.2 years, 72% Caucasians, 12% Mulattoes and 12% Asians, were submitted to serum aminotransferase and iron profile determinations. Liver biopsies were analyzed for necroinflammatory activity, architectural damage and iron deposition. In 31 of the patients, C282Y and H63D mutations were tested by PCR-RFLP. Alanine aminotransferase levels were increased in 30 patients, 2.42 ± 1.12 times the upper normal limit on average. Serum iron concentration, transferrin saturation and ferritin averages were 99.4 ± 31.3 g/dl, 33.1 ± 12.7% and 219.8 ± 163.8 µg/dl, respectively, corresponding to normal values in 93.5, 68.7 and 78.1% of the patients. Hepatic siderosis was observed in three patients and was not associated with architectural damage (P = 0.53) or with necroinflammatory activity (P = 0.27). The allelic frequencies (N = 31) found were 1.6 and 14.1% for C282Y and H63D, respectively, which were compatible with those described for the local population. In conclusion, no evidence of an association of hepatic iron overload and HFE mutations with NASH was found. Brazilian NASH patients comprise a heterogeneous group with many associated conditions such as hyperinsulinism, environmental hepatotoxin exposure and drugs, but not hepatic iron overload, and their disease susceptibility could be related to genetic and environmental features other than HFE mutations