Dimensionamento de sistemas de tratamento (decantador e lagoas) e utilização de dejetos suínos.

Características dos dejetos; Equalizador; Decantador; Lagoas de tratamento; Adubação orgânica.bitstream/item/58411/1/CUsersPiazzonDocuments234.pd

Avaliação dos métodos de suscetibilidade antifúngica: Etest e macrodiluição em caldo para leveduras do gênero Candida isoladas da cavidade bucal de pacientes com AIDS

A comparison of the Etest and the reference broth macrodilution susceptibility test for fluconazole, ketoconazole, itraconazole and amphotericin B was performed with 59 of Candida species isolated from the oral cavities of AIDS patients. The Etest method was performed according to the manufacturer's instructions, and the reference method was performed according to National Committee for Clinical Laboratory Standards document M27-A guidelines. Our data showed that there was a good correlation between the MICs obtained by the Etest and broth dilution methods. When only the MIC results at ± 2 dilutions for both methods were considered, the agreement rates were 90.4% for itraconazole, ketoconazole and amphotericin B and 84.6% for fluconazole of the C. albicans tested. In contrast, to the reference method, the Etest method classified as susceptible three fluconazole-resistant isolates and one itraconazole-resistant isolate, representing four very major errors. These results indicate that Etest could be considered useful for antifungal sensitivity evaluation of yeasts in clinical laboratories.Foram comparados dois testes de suscetibilidade in vitro, macrodiluição em caldo e Etest através da determinação das concentrações inibitórias mínimas de fluconazol, cetoconazol, itraconazol e anfotericina B para 59 isolados de Candida obtidos da cavidade bucal de pacientes com AIDS. O método de Etest foi feito de acordo com as instruções do fabricante e o método de macrodiluição em caldo foi realizado seguindo as normas do NCCLS (National Committee for Clinical Laboratoratory Standards), documento M27-A, considerado método de referência. Nossos dados mostraram uma boa correlação entre os valores de concentração inibitória mínima encontrados para o Etest e o método de macrodiluição em caldo. Foi verificada concordância entre os resultados de CIM, de 90,4% para itraconazol, cetoconazol e anfotericina B e de 84,6% para fluconazol, quando se analisou isolados de C. albicans. A comparação de breakpoints mostrou que havia quatro grandes discrepâncias de resultados entre os dois métodos, sendo que pelo método de referência três isolados de Candida albicans classificados como resistentes ao fluconazol e um de C. krusei considerado resistente ao itraconazol foram sensíveis ao Etest. Estes resultados mostraram que o Etest é de grande valor nos testes de suscetibilidade antifúngica para leveduras do gênero Candida

Milhocultores optam por híbridos com alto teto produtivo.

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Antioxidant Effects of a Hydroxytyrosol-Based Pharmaceutical Formulation on Body Composition, Metabolic State, and Gene Expression: A Randomized Double-Blinded, Placebo-Controlled Crossover Trial

Hydroxytyrosol (HT) plays a significant role in cardiovascular disease (CVD) protection, and its metabolites are able to protect from the endothelial dysfunction commonly present in atherosclerosis. This randomized double-blinded, placebo-controlled crossover trial determined the effect in healthy volunteers of two gastroresistant capsules containing 15 mg/day of HT, for a 3-week period (HTT). Evaluation of nutritional status, serum metabolites, oxidative stress biomarkers, and gene expression of 9 genes related to oxidative stress, inflammation, and CVDs was performed. Oxidation biomarkers like thiol group (p = 0.001), total antioxidant status (TAS) (p = 0.001), superoxide dismutase 1 (SOD1) (2−ΔΔCt = 3.7), and plasma concentration of HT (2.83 μg·mL−1) were significantly increased,while nitrite (p = 0.001), nitrate (p = 0.001), and malondialdehyde (MDA) (p = 0.02), were drastically reduced after HTT. A significant reduction of body fat mass percentage (p = 0.01), suprailiac skinfold (p = 0.01), and weight (p = 0.04; Δ% = −0.46%) was observed after HTT. This study shows that regular intake of 15 mg/day of HT changed body composition parameters and modulated the antioxidant profile and the expression of inflammation and oxidative stress-related genes. However, it is advisable to personalize HT doses in order to exert its health benefits in CVD prevention and protection of LDL-C particles from oxidative damage. This trial is registered with ClinicalTrials.gov NCT01890070

Renewed global partnerships and redesigned roadmaps for rabies prevention and control

Canine rabies, responsible for most human rabies deaths, is a serious global public health concern. This zoonosis is entirely preventable, but by focusing solely upon rabies prevention in humans, this "incurable wound" persists at high costs. Although preventing human deaths through canine rabies elimination is feasible, dog rabies control is often neglected, because dogs are not considered typical economic commodities by the animal health sector. Here, we demonstrate that the responsibility of managing rabies falls upon multiple sectors, that a truly integrated approach is the key to rabies elimination, and that considerable progress has been made to this effect. Achievements include the construction of global rabies networks and organizational partnerships; development of road maps, operational toolkits, and a blueprint for rabies prevention and control; and opportunities for scaling up and replication of successful programs. Progress must continue towards overcoming the remaining challenges preventing the ultimate goal of rabies elimination

Mapeamento da Ocorrência do Mal-do-Panamá em Rondônia.

Em Rondônia, o Mal-do-Panamá apresenta-se juntamente com a Sigatoka Negra e Amarela as doenças de maior relevância da bananicultura. Foi detectado Mal-doPanamá em diferentes municípios do Estado, tais como Mirante da Serra, Governador Jorge Teixeira, São Miguel do Guaporé, Machadonho do Oeste e Alvorada do Oeste, dentre outros. A doença é causada pelo fungo Fusarium oxysporum f.sp. cubense (E.F. Smith) Sn & Hansen. Este fungo pertence à classe dos deuteromicetos e caracteriza-se pela grande capacidade de sobrevivência na ausência do hospedeiro, permanecendo no solo por longos períodos de tempo...bitstream/CPAF-RO-2010/13273/1/cot330-maldopanama.pd

Sigatoka Negra em Rondônia.

O presente trabalho visa apresentar dados sobre a ocorrência da sigatoka negra nos diferentes municípios do Estado de Rondônia, tendo como base os dados obtidos a partir do mapeamento da ocorrência de sigatoka negra realizado pela Embrapa Rondônia em parceria com a Agência de Defesa Agrossilvopastoril de Rondônia - Idaron, o qual teve início em 2004. Os dados apresentados representam coletas realizadas no período de janeiro de 2004 a junho de 2007 por técnicos da agência Idaron nos diferentes municípios do Estado.bitstream/CPAF-RO-2009-09/12229/1/cot324_sigatokanegra.pd