2,741 research outputs found
Detection of Toxoplasma gondii in the milk of experimentally infected Wistar female rats
The milk of experimentally infected rats was investigated for the presence and possible transmission of Toxoplasma gondii. Wistar (Rattus norvegicus) female rats were divided into three groups and orally inoculated with bradyzoites. Polymerase chain reaction (PCR) was employed to detect the parasite in the milk. Transmission to the offspring was verified by indirect immunofluorescence antibody test (IFAT), modified agglutination test (MAT), bioassay tests and PCR. Rat milk samples were PCR-positive, pups were serum-reactive to T. gondii and tissue samples also presented positive DNA results through PCR
Biological effects of soft denture reline materials on L929 cells in vitro.
Soft denture reline materials have been developed to help patients when their oral mucosa is damaged or affected due to ill-fitting dentures or post-implant surgery. Although reports have indicated that these materials leach monomers and other components that do affect their biocompatibility, there is little information on what cell molecules may be implicated in these material/tissue interactions. The biocompatibility of six soft liners (Ufi Gel P, Sofreliner S, Durabase Soft, Trusoft, Softone and Coe Comfort) was evaluated using a mouse fibroblast cell line, L929. Within 2 h of material disc preparation, each of the materials was exposed by direct contact to L929 cells for periods of 24 and 48 h. The effect of this interaction was assessed by alamarBlue assay (for cell survival). The expression of integrin α5β1 and transforming growth factor β1 was also assessed using plate assays such as enzyme-linked immunosorbent assay. Trusoft, Softone and Coe Comfort showed significantly reduced cell survival compared with the other soft lining materials at each incubation period. Furthermore, there were significant differences with these same materials in the expression of both integrin α5β1 and transforming growth factor β1. Soft liner materials may affect cell viability and cellular proteins that have important roles in wound healing and the preservation of cell viability and function in the presence of environmental challenges and stresses
Can the curriculum be used to estimate critical velocity in young competitive swimmers?
The aims of the present study were to assess critical velocity using the swimmer curriculum in front crawl events and to compare critical velocity to the velocity corresponding to a 4 mmol·l(-1) of blood lactate concentration and to the velocity of a 30 min test. The sample included 24 high level male swimmers ranged between 14 and 16 years old. For each subject the critical velocity, the velocity corresponding to a 4 mmol·l(-1) of blood lactate concentration and the mean velocity of a 30 min test were determined. The critical velocity was also estimated by considering the best performance of a swimmer over several distances based on the swimmer curriculum. Critical velocity including 100, 200 and 400 m events was not different from the velocity of 4 mmol·l(-1) of blood lactate concentration. Critical velocity including all the swimmer events was not different from the velocity of a 30 min test. The assessment of critical velocity based upon the swimmer curriculum would therefore seem to be a good approach to determine the aerobic ability of a swimmer. The selection of the events to be included in critical velocity assessment must be a main concern in the evaluation of the swimmer. Key pointsCritical velocity using 100, 200 and 400 m events was not different from the velocity of 4 mmol·l(-1) of blood lactate concentration.Critical velocity using all the swimmer events was not different from the velocity of a 30 min test.The assessment of critical velocity based upon the swimmer curriculum seemed to be a good approach to determine the aerobic capacity of a swimmer.The decision on the events to be analysed must be a main concern in the evaluation of the swimmer critical velocity.info:eu-repo/semantics/publishedVersio
Survival Analysis of COPD Patients in a 13-Year Nationwide Cohort Study of the Brazilian National Health System
Background: Chronic obstructive pulmonary disease (COPD) has an appreciable socioeconomical impact in low- and middle-income countries, but most epidemiological data originate from high-income countries. For this reason, it is especially important to understand survival and factors associated with survival in COPD patients in these countries. Objective: To assess survival of COPD patients in Brazil, to identify risk factors associated with overall survival, including treatment options funded by the Brazilian National Health System (SUS). Methodology: We built a retrospective cohort study of patients dispensed COPD treatment in SUS, from 2003 to 2015 using a National Database created from the record linkage of administrative databases. We further matched patients 1:1 based on sex, age and year of entry to assess the effect of the medicines on patient survival. We used the Kaplan-Meier method to estimate overall survival of patients, and Cox's model of proportional risks to assess risk factors. Result: Thirty seven thousand and nine hundred and thirty eight patients were included. Patient's survival rates at 1 and 10 years were 97.6% (CI 95% 97.4–97.8) and 83.1% (CI 95% 81.9–84.3), respectively. The multivariate analysis showed that male patients, over 65 years old and underweight had an increased risk of death. Therapeutic regimens containing a bronchodilator in a free dose along with a fixed-dose combination of corticosteroid and bronchodilator seem to be a protective factor when compared to other regimens. Conclusion: Our findings contribute to the knowledge of COPD patients' profile, survival rate and related risk factors, providing new evidence that supports the debate about pharmacological therapy and healthcare of these patients
Does combined dry land strength and aerobic training inhibit performance of young competitive swimmers?
The aim of the current study was twofold: (i) to examine the
effects of eight weeks of combined dry land strength and aerobic
swimming training for increasing upper and lower body
strength, power and swimming performance in young competitive
swimmers and, (ii) to assess the effects of a detraining
period (strength training cessation) on strength and swimming
performance. The participants were divided into two groups: an
experimental group (eight boys and four girls) and a control
group (six boys and five girls). Apart from normal practice
sessions (six training units per week of 1 h and 30 min per day),
the experimental group underwent eight weeks (two sessions per
week) of strength training. The principal strength exercises were
the bench press, the leg extension, and two power exercises such
as countermovement jump and medicine ball throwing. Immediately
following this strength training program, all the swimmers
undertook a 6 week detraining period, maintaining the normal
swimming program, without any strength training. Swimming
(25 m and 50 m performances, and hydrodynamic drag values),
and strength (bench press and leg extension) and power (throwing
medicine ball and countermovement jump) performances
were tested in three moments: (i) before the experimental period,
(ii) after eight weeks of combined strength and swimming
training, and (iii) after the six weeks of detraining period. Both
experimental and control groups were evaluated. A combined
strength and aerobic swimming training allow dry land strength
developments in young swimmers. The main data can not
clearly state that strength training allowed an enhancement in
swimming performance, although a tendency to improve sprint
performance due to strength training was noticed. The detraining
period showed that, although strength parameters remained
stable, swimming performance still improved.info:eu-repo/semantics/publishedVersio
Validação conceitual das características definidoras de diagnósticos de enfermagem respiratórios em neonatos
OBJECTIVE:To develop and validate conceptual and operational definitions for the defining characteristics of the respiratory nursing diagnoses, ineffective breathing pattern, impaired gas exchange and impaired spontaneous ventilation, in newborns.METHODS:This was a methodological study of conceptual validation of the defining characteristics of three respiratory nursing diagnoses, by consensus analysis of a committee of five specialist nurses, and then a group of five non-nursing professionals, using the Delphi technique.RESULTS:After two rounds of evaluation, consensus was obtained that was equal to or greater than 80% on all of the definitions, which were then considered validated.CONCLUSION:The definitions developed for the defining characteristics of three nursing diagnoses were validated with a high level of consensus.OBJETIVO:Elaborar e validar definições conceituais e operacionais para as características definidoras dos diagnósticos de enfermagem respiratórios, Padrão Respiratório Ineficaz, Troca de Gases Prejudicada e Ventilação Espontânea Prejudicada em recém-nascidos.MÉTODOS:Estudo metodológico, de validação conceitual das características definidoras dos três diagnósticos de enfermagem respiratórios por meio da análise de consenso de um comitê de cinco enfermeiras especialistas e de cinco profissionais não enfermeiros, utilizando a técnica Delphi.RESULTADOS:Após duas rodadas de avaliação, obteve-se consenso igual ou superior a 80% na totalidade das definições, sendo consideradas validadas.CONCLUSÃO:As definições elaboradas para as características definidoras dos três diagnósticos de enfermagem foram validadas com elevado grau de consenso.Universidade Federal de São Paulo (UNIFESP) Escola Paulista de EnfermagemUNIFESP, EPESciEL
The use of neural network technology to model swimming performance
to identify the factors which are able to explain the performance in the 200 meters individual medley and 400 meters front crawl events in young swimmers, to model the performance in those events using non-linear mathematic methods through artificial neural networks (multi-layer perceptrons) and to assess the neural network models precision to predict the performance. A sample of 138 young swimmers (65 males and 73 females) of national level was submitted to a test battery comprising four different domains: kinanthropometric evaluation, dry land functional evaluation (strength and flexibility), swimming functional evaluation (hydrodynamics, hydrostatic and bioenergetics characteristics) and swimming technique evaluation. To establish a profile of the young swimmer non-linear combinations between preponderant variables for each gender and swim performance in the 200 meters medley and 400 meters font crawl events were developed. For this purpose a feed forward neural network was used (Multilayer Perceptron) with three neurons in a single hidden layer. The prognosis precision of the model (error lower than 0.8% between true and estimated performances) is supported by recent evidence. Therefore, we consider that the neural network tool can be a good approach in the resolution of complex problems such as performance modeling and the talent identification in swimming and, possibly, in a wide variety of sports. Key pointsThe non-linear analysis resulting from the use of feed forward neural network allowed us the development of four performance models.The mean difference between the true and estimated results performed by each one of the four neural network models constructed was low.The neural network tool can be a good approach in the resolution of the performance modeling as an alternative to the standard statistical models that presume well-defined distributions and independence among all inputs.The use of neural networks for sports sciences application allowed us to create very realistic models for swimming performance prediction based on previous selected criterions that were related with the dependent variable (performance).info:eu-repo/semantics/publishedVersio
ISSR markers for genetic relationships in Caricaceae and sex differentiation in papaya
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