12 research outputs found

    Bioprospecção de bacterias produtoras de polihidroxialcanoatos a partir de oleo de andiroba / Bioprospecting of bacteria producing polyhydroxyalkanoates from andiroba oil

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    O objetivo desse estudo foi a bioprospecção de bactérias produtoras de polihidroxialcanoatos. Amostras de óleo de andiroba foram cedidas pela COOPERAR. As amostras foram plaqueadas em meio TSB. As colônias foram analisadas quanto a presença de PHAs utilizando a coloração Sudan Black. O DNA das colônias bacterianas positivas para a produção de PHA foi extraído com a utilização de kits de extração. Após extração de DNA as amostras foram submetidas a PCR e algumas amostras foram enviadas para sequenciamento genético. Das 250 colônias obtidas em meio TSB, 110 mostrara-se positivas para a produção de PHA através da coloração Sudan Black. Das 110 colônias, foram realizadas extrações de DNA de 10 amostras. E as 3 melhores amostras foram sequenciadas. No sequenciamento genético os seguintes gêneros foram identificados: Bacillus subtilis, Enterococccus faeccium e Proteus mirabilis. Visto que o gênero Proteus relatado pela primeira vez como produtor de polihidroxialcanoatos

    Uso das Pironofatoquinona como antiviral; composição farmacêutica contendo as Piranonaftoquinonas; medicamento contendo as Piranonaftoquinonas para tratamento de infecções causadas por virus da dengue

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    DepositadaDescreve o uso das Piranonaftoquinonas, assim como seus derivados, seus isômeros e seus sais como um agente antiviral, por inibir as enzimas ATPases viral. Além disso, também, faz parte desta invenção uma composição farmacêutica contendo as Piranonaftoquinonas descritas

    Compostos peptídeos miméticos derivados do ácido tartárico potencialmente ativos contra vírus da Hepatite C e composição farmacêutica contendo tais compostos

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    DepositadaA presente invenção se refere a compostos peptídeo mimético derivados do ácido tartárico potencialmente ativos contra vírus da Hepatite C e composição farmacêutica contendo tais compostos, desenhados como inibidores de polimerases e serina protease do vírus da Hepatite C (HCV), sintéticos, caracterizados por possuir uma estrutura do tipo 1,4:3,6-dianidromanitol e um cerne tipo ácido tartárico. As porções laterais são caracterizadas por possuir ligações peptídeos miméticas provenientes do acoplamento com diversos aminoésteres

    Effects of pH on NS3 binding to ssDNA.

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    <p>Increasing protein concentrations (from 0 to 10 µM NS3hel and 0 to 5 µM NS3FL) were used to compare NS3hel (A) and NS3FL (B) binding to a fluorescently-labeled ssDNA at pH 6.4 and 7.2 and to calculate the dissociation constants (K<sub>d</sub>) between ssDNA and the constructs. Closed (pH 6.4) and open circles (pH 7.2) represent the mean of fluorescence anisotropy obtained in three independent experiments. Data were obtained at 25°C and assay buffers contained 25 mM MOPS-NaOH (pH 6.4 or 7.2), 2 mM MgCl<sub>2</sub> and 25 nM of the fluorescently labeled ssDNA.</p

    Effects of ssDNA binding on NS3 structure monitored by Trp fluorescence quenching and bis-ANS binding.

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    <p>Increasing ssDNA concentrations (from 0 to 1 µM) were used to compare the Trp fluorescence quenching of NS3hel and NS3FL (A and B, respectively) and the bis-ANS binding to these proteins (C and D, respectively) at pH 6.4 (closed circles) and 7.2 (open circles). Each point represents the mean of Trp fluorescence quenching or bis-ANS binding obtained in three independent experiments. Spectra were obtained at 25°C and assay buffers contained 25 mM MOPS-NaOH (pH 6.4 or 7.2), 2 mM MgCl<sub>2</sub> and 1 µM of purified proteins.</p

    Fluorescence quenching of NS3 Trp residues by acrylamide at pH 6.4 and 7.2.

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    <p>Acrylamide concentrations ranging from 0 to 156 mM were used to monitor the exposure of the Trp residues of NS3hel (A) and NS3FL (B) at pH 6.4 (closed circles) and 7.2 (open circles) and to calculate the Stern-Volmer constant (K<sub>sv</sub>) using <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0115941#pone.0115941.e003" target="_blank">Equation 3</a> (<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0115941#s4" target="_blank">Material and Methods</a>). Each point corresponds to the mean of tryptophan fluorescence quenching by acrylamide obtained in three independent experiments. Spectra were acquired at 25°C in buffer solutions composed of 50 mM MOPS-NaOH (pH 6.4 or 7.2), 200 mM NaCl, 5 mM β-mercaptoethanol and 5% glycerol. The protein concentration was 1 µM.</p

    Effects of bis-ANS binding on ATPase activity at different pHs.

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    <p>Increasing bis-ANS concentrations (from 0 to 300 µM) were used to compare the effect of bis-ANS binding on the ATPase activity of NS3hel (A) and NS3FL (B). Closed (pH 6.4) and open circles (pH 7.2) represent the mean of the residual ATPase activity obtained in three independent experiments and bars indicate the standard error. Reactions were performed at 30°C during 60 min using 40 mM MES-KOH (pH 6.4) or Tris-HCl (pH 7.2), 5 mM DTT, 5 mM MgCl<sub>2</sub>, 100 mM KCl, 1 mM ATP and 0.1 µM of purified proteins. IC<sub>50</sub> values were calculated using <i>Sigma plot</i> ver. 10.0 after plotting the dose-response curve of bis-ANS concentration versus residual ATPase activity.</p

    Analysis of the pH effects on the NS3 secondary structure upon chemical denaturation.

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    <p>A) CD spectra of 10 µM NS3hel acquired at pH 7.2 in the absence of Gdn.HCl (solid line), or presence of 2.5 M (dashed line) and 5 M (dotted line) Gdn.HCl. The spectra were the average of three scans after subtracting the buffer baselines. Each spectrum was converted into molar ellipticity using <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0115941#pone.0115941.e004" target="_blank">Equation 4</a> (<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0115941#s4" target="_blank">Material and Methods</a>). B) The ellipticity values at 222 nm (θ<sub>222</sub>) at each Gdn.HCl concentration (from 0 to 5 M) were used to compare the secondary structure stability of NS3hel at pH 6.4 and 7.2 and to calculate the degree of denaturation using <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0115941#pone.0115941.e005" target="_blank">Equation 5</a> (<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0115941#s4" target="_blank">Material and Methods</a>). Closed (pH 6.4) and open circles (pH 7.2) represent the degree of denaturation at each Gdn.HCl concentration. Spectra were acquired at 25°C in buffer solutions composed of 50 mM MOPS-NaOH (pH 6.4 or 7.2), 200 mM NaCl, 5 mM β-mercaptoethanol and 5% glycerol. The protein concentration was 10 µM.</p

    Interaction of the fluorescent extrinsic probe bis-ANS with NS3 at pH 6.4 and 7.2.

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    <p>bis-ANS concentrations ranging from 0 to 8 µM were used to compare NS3hel (A) and NS3FL (B) hydrophobic clefts exposure at pH 6.4 (closed circles) and 7.2 (open circles). The inset in the graph A shows a reduction in the y-axis scale to demonstrate more clearly the effect of increasing bis-ANS fluorescence at both pH. Each point corresponds to the mean of the normalized bis-ANS fluorescence intensity obtained in three independent experiments. Spectra were acquired at 25°C in buffer solutions composed of 50 mM MOPS-NaOH (pH 6.4 or 7.2), 200 mM NaCl, 5 mM β-mercaptoethanol and 5% glycerol. The protein concentration was 1 µM.</p

    Use of medication and associated factors in adults living in Rio Branco, Acre. Use of medication in adults

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    We analyzed use of medication and associated factors in adults aged 18-59 years living in Rio Branco, Acre. This is a cross-sectional and population-based study that used a probabilistic sample of the population from rural and urban areas of the city of Rio Branco, Acre. The Prevalence Ratio (PR) was calculated with 95% confidence intervals and associations were estimated by Poisson regression. This study found a 29.4% prevalence ratio of use of medication among individuals aged from 18 to 59 years (685 adults: 473 women and 212 men; producing estimates for 211,902 adults: 110,769 women and 101,133 men). After adjusted analysis, their use was associated with: age (50-59 years, PR: 2.36; 95%CI: 2.29-2.43); women (PR: 1.25; 95%CI: 1.23-1.27); up to elementary school (PR: 1.13; 95%CI: 1.11-1.15); and poor or very poor self-rated health (PR: 1.47; 95%CI: 1.43-1.51). The health conditions associated with use of medication were: number of comorbidities, hypertension, diabetes, insomnia, depression, number of health complaints and use of health services. The most frequently used drugs were those belonging to the following ATC categories: alimentary tract and metabolism, cardiovascular system, nervous system, and the musculoskeletal system
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