THE SYNTHESIS OF SPHERICAL SHAPE AMINO FUNCTIONALIZED PEI-TGIC COVALENT ORGANIC FRAMEWORKS: SYNTHESIS, CHARACTERIZATION, AND METHYL ORANGE ABSORPTION

In here, the synthesis of covalent organic framework (COF) from the reaction of polyethyleneimine (PEI) and triglycidyl isocyanurate (TGIC) in dimethylformamide at 90 oC were carried out. The surface are, pore volume and pore size values of PEI-TGIC COFs observed as 23.4 m2/g, 0.143 cm3/g, and 22.5 nm, respectively. Moreover, the surface charge of PEI-TGIC COFs increased to +46.1±2.6 mV from +18.3±1.7 mV, after protonation of amine groups of PEI-TGIC COFs. Moreover, the potential usage of PEI-TGIC based COFs in absorption methyl orange (MO) dye from aqueous media was testing. It was observed that, the PEI-TGIC COF absorbed 156.6±4.9 mg/g MO, and the protonated PEI-TGIC (p-PEI-TGIC) COFs absorbed 202.4±5.3 mg/g MO from aqueous media in 30 min. The MO absorption by PEI-TGIC COF fitted with pseudo-first-order kinetic model, whereas MO absorption by p-PEI-TGIC COF fitted with pseudo-second-order kinetic model

P (Tannic Acid) hydrogel template for in situ metal nanoparticle preparation and use as catalyst for elimination of toxic organic compounds

European Biotechnology Conference -- MAY 05-07, 2016 -- LATVIAWOS: 00038024030029

Blau syndrome with a rare mutation in exon 9 of NOD2 gene

Blau syndrome is an autosomal dominant rare disease caused by mutations in NOD2 gene. Less than 200 patients published with Blau Syndrome Worldwide. We reported a 41-year old female Turkish patient diagnosed as Blau syndrome. Granulomatous dermatitis and severe headache, as well as recurrent chest and pelvic pain have been present since she was 8 years old. Arthritis started when she was teenage, hypertension diagnosed when she was 20 and other symptoms also occurred during the lifetime (severe preeclampsia, ischemic stroke, recurrent hemiparesis, recurrent-transient-vision-loss and renal-artery-stenosis). Genomic DNA was isolated from peripheral blood and 12 genes sequenced in Autoinflammatory panel on IonTorrent-S5-NGS platform with Parseq-VariFind™AIPassay. NGS analysis showed 107 variants in in the index case, mainly benign with no strong association with Blau syndrome. Additionally, we identified one very rare missense mutation in NOD2 gene (c2803G>A, p.Val935Met) and in silico assessment of the mutation indicated possible pathogenic significance and strong association with Blau syndrome. In addition, we analyzed family members of the index case and identified the same mutation in NOD2 gene. The segregation analysis shows the presence of the same mutant allele in NOD2 gene in the index case affected sister, as well as in her son with arthralgia, while in her non affecter brother we didn’t detect the Val935Met mutation in NOD2 gene. Blau Syndrome is known as a very rare disease, mainly caused by mutations in NOD2 gene. Missense mutation diagnosed in our case could be responsible for the phenotype of the index case. Our results indicate the importance of NGS testing and its major role in the detection of rare mutations that may responsible for the onset of autoinflammatory disorders

P(TA) macro-, micro-, nanoparticle-embedded super porous p(HEMA) cryogels as wound dressing material

WOS: 000388046900039PubMed ID: 27770897Super porous poly(2-hydroxy ethyl methacrylate) (p(HEMA)) cryogel was successfully synthesized by using polyethylene glycol diacrylate (p(EGDA)) crosslinker under cryogenic conditions. Poly(Tannic acid) (p(TA)) macro-, micro-, and nanopartides prepared from a natural polyphenol, tannic acid (TA), were embedded into p(HEMA) cryogel networks to obtain composite p(TA) particle-embedded p(HEMA) cryogel. Different size ranges of spherical p(TA) particles, 2000-500 mu m, 500-200 mu m, 200-20 mu m, and 20-0.5 mu m size, were included in the cryogel network and illustrated by digital camera, optic microscope, and SEM images of the microgelcryogel network. The swelling properties and moisture content of p(TA) microgel-embedded p(HEMA) cryogel were investigated at wound healing pH conditions such as pH 5.4, 7A, and 9 at 37.5 degrees C, and the highest swelling capacity was found at pH 9 with 972 +/- 2% swelling in 30 s. Higher amounts of DI water were quickly absorbed by p(HEMA)-based cryogel, and moisture retention within the cryogel structure for a longer time period at room temperature is due to existence of p(TA) particles. Degradation profiles of p(TA) particle-embedded p(HEMA) cryogel were shown to be controlled by different pH conditions, and a linear release profile was found with total cumulative release of 5.8 +/- 0.8 mg/g TA up to 12 days at pH 7A and 37.5 degrees C. The antioxidant behavior of degraded p(TA) particles from p(HEMA) cryogel were found as 46 +/- 1 mu g mL(-1) gallic acid equivalent and 165 +/- 18 mM trolox equivalent g(-1). The p(TA) particle-embedded p(HEMA) cryogel has high hemocompatibility with 0.0158 +/- 0.0126% hemolysis ratio, and effective hemostatic properties with 8.1 +/- 0.9 blood clotting index. (C) 2016 Elsevier B.V. All rights reserved.Scientific and Technological Research Council of TurkeyTurkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) [113Z238]; Canakkale Onsekiz Mart UniversityCanakkale Onsekiz Mart University [TSA-2014-362]This work is supported by the Scientific and Technological Research Council of Turkey (113Z238). Also, financial support from Canakkale Onsekiz Mart University, TSA-2014-362 is greatly appreciated

Psychoactive Drug Related Traumatic Deaths in Istanbul between 1990-2000

The objective of this study was to identify characteristics of drug related traumatic deaths in Istanbul between 1990 and 2000. This retrospective descriptive study was set in the Council of Forensic Medicine, Ministry of Justice. During the period studied 143 people (86 % males, 14 % females) who took a drug died after traumatic events. The mean age was 37.7 +/- 13.6 years. The most common causes of deaths were asphyxia and blunt force injury. Benzodiazepines, cannabis and heroin were the most commonly used drugs. While benzodiazepines are common in self-directed violence cases such as suicide by asphyxia, cannabis was frequent among interpersonal violence cases as homicide by shooting. Heroin was approximately equally seen in all traumatic deaths. Benzodiazepine and heroin use are especially frequent among non-violence deaths (road traffic accidents)

Biocompatible and biodegradable poly(Tannic Acid) hydrogel with antimicrobial and antioxidant properties

WOS: 000367425100020PubMed ID: 26526171A novel resourceful bulk poly(Tannic Acid) (p(TA)) hydrogel was prepared by crosslinking TA molecules with an epoxy crosslinker, trimethylolpropane triglycidyl ether (TMPGDE), in an autoclave at 90 degrees C for 2 h. The obtained p(TA) hydrogels were in disk form and have highly porous morphology. The swelling characteristics of p(TA) hydrogels were investigated in wound healing pH conditions of pH 5.4, 7.4, and 9 at 37.5 degrees C, and the hydrogels showed good swelling and moisture content behavior. Especially, p(TA) hydrogels were found to be sensitive to pH 9 with 1669% maximum swelling. P(TA) hydrogels were completely degraded at pH 9 hydrolytically in 9 days. Total phenol contents and the effects of scavenging ABTS radicals of degraded p(TA) hydrogels at pH 5.4, 7.4, and 9 were evaluated and calculated in terms of gallic acid equivalent and trolox equivalent antioxidant capacity, respectively, and found to be very effective. Moreover, degraded p(TA) hydrogels display strong antimicrobial behavior against gram positive Staphylococcus aureus, Bacillus subtilis, gram negative Pseudomonas aeruginosa bacteria strains and Candida albicans fungus strain. The WST-1 results indicated that bulk p(TA) hydrogels have no cyctotoxicity to the L929 fibroblast cell line in vitro. (C) 2015 Elsevier B.V. All rights reserved.Scientific and Technological Research Council of TurkeyTurkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) [113Z238]This work is supported by The Scientific and Technological Research Council of Turkey (113Z238)

Attenuation of partial unilateral ureteral obstruction – induced renal damage with hyperbaric oxygen therapy in a rat model

ABSTRACT Objective: The objective of the present study was to evaluate the effectiveness of HBO therapy on biochemical parameters, renal morphology and renal scintigraphy in rats undergoing chronic unilateral partial ureteral obstruction (UPUO). Material and methods: Thirty-five rats were divided into five equal groups: Control group; Sham group; HBO group; UPUO group and UPUO/HBO group. The effects of HBO therapy were examined using biochemical parameters and histopathological changes. After calculating the score for each histopathological change, the total histopathological score was obtained by adding all the scores. In addition, dynamic renal scintigraphy findings were evaluated. Results: Serum parameters indicating inflammation, serum tumor necrosis factor- alpha, ischemia modified-albumin, IMA/albumin ratio and Pentraxin-3 levels, were observed to be high in the UPUO group and low in the UPUO/HBO treatment group. Similarly, in the treatment group, the reduction in malondialdehyde, total oxidant status and oxidative stress index levels and increase in total antioxidant capacity values were observed to be statistically significant compared to the UPUO group (p<0.001, p=0.007, p<0.001, p=0.001, respectively). The total score and apoptosis index significantly decreased after administration of HBO treatment. Dynamic 99mTc-MAG3 renal scintigraphy also showed convincing evidence regarding the protective nature of HBO against kidney injury. In the UPUO/HBO therapy group, the percentage contribution of each operated kidney increased significantly compared to the UPUO group (41.73% versus 32.72%). Conclusion: The findings of this study indicate that HBO therapy had a reno-protective effect by reducing inflammation and oxidative stress, and preserving renal function after renal tissue damage due to induction of UPUO

Attenuation of partial unilateral ureteral obstruction – induced renal damage with hyperbaric oxygen therapy in a rat model

<div><p>ABSTRACT Objective: The objective of the present study was to evaluate the effectiveness of HBO therapy on biochemical parameters, renal morphology and renal scintigraphy in rats undergoing chronic unilateral partial ureteral obstruction (UPUO). Material and methods: Thirty-five rats were divided into five equal groups: Control group; Sham group; HBO group; UPUO group and UPUO/HBO group. The effects of HBO therapy were examined using biochemical parameters and histopathological changes. After calculating the score for each histopathological change, the total histopathological score was obtained by adding all the scores. In addition, dynamic renal scintigraphy findings were evaluated. Results: Serum parameters indicating inflammation, serum tumor necrosis factor- alpha, ischemia modified-albumin, IMA/albumin ratio and Pentraxin-3 levels, were observed to be high in the UPUO group and low in the UPUO/HBO treatment group. Similarly, in the treatment group, the reduction in malondialdehyde, total oxidant status and oxidative stress index levels and increase in total antioxidant capacity values were observed to be statistically significant compared to the UPUO group (p<0.001, p=0.007, p<0.001, p=0.001, respectively). The total score and apoptosis index significantly decreased after administration of HBO treatment. Dynamic 99mTc-MAG3 renal scintigraphy also showed convincing evidence regarding the protective nature of HBO against kidney injury. In the UPUO/HBO therapy group, the percentage contribution of each operated kidney increased significantly compared to the UPUO group (41.73% versus 32.72%). Conclusion: The findings of this study indicate that HBO therapy had a reno-protective effect by reducing inflammation and oxidative stress, and preserving renal function after renal tissue damage due to induction of UPUO.</p></div