21 research outputs found

    SALDI-MS and SERS Multimodal Imaging: One Nanostructured Substrate to Rule Them Both

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    Imaging techniques based on mass spectrometry or spectroscopy methods inform in situ about the chemical composition of biological tissues or organisms, but they are sometimes limited by their specificity, sensitivity, or spatial resolution. Multimodal imaging addresses these limitations by combining several imaging modalities; however, measuring the same sample with the same preparation using multiple imaging techniques is still uncommon due to the incompatibility between substrates, sample preparation protocols, and data formats. We present a multimodal imaging approach that employs a gold-coated nanostructured silicon substrate to couple surface-assisted laser desorption/ionization mass spectrometry (SALDI-MS) and surface-enhanced Raman spectroscopy (SERS). Our approach integrates both imaging modalities by using the same substrate, sample preparation, and data analysis software on the same sample, allowing the coregistration of both images. We transferred molecules from clean fingertips and fingertips covered with plasticine modeling clay onto our nanostructure and analyzed their chemical composition and distribution by SALDI-MS and SERS. Multimodal analysis located the traces of plasticine on fingermarks and provided chemical information on the composition of the clay. Our multimodal approach effectively combines the advantages of mass spectrometry and vibrational spectroscopy with the signal enhancing abilities of our nanostructured substrate

    (1)H-NMR-based metabolomic analysis of the effect of moderate wine consumption on subjects with cardiovascular risk factors

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    Moderate wine consumption is associated with health-promoting activities. An H-NMR-based metabolomic approach was used to identify urinary metabolomic differences of moderate wine intake in the setting of a prospective, randomized, crossover, and controlled trial. Sixty-one male volunteers with high cardiovascular risk factors followed three dietary interventions (28 days): dealcoholized red wine (RWD) (272mL/day, polyphenol control), alcoholized red wine (RWA) (272mL/day) and gin (GIN) (100mL/day, alcohol control). After each period, 24-h urine samples were collected and analyzed by (1) H-NMR. According to the results of a one-way ANOVA, significant markers were grouped in four categories: alcohol-related markers (ethanol); gin-related markers; wine-related markers; and gut microbiota markers (hippurate and 4-hydroxphenylacetic acid). Wine metabolites were classified into two groups; first, metabolites of food metabolome: tartrate (RWA and RWD), ethanol, and mannitol (RWA); and second, biomarkers that relates to endogenous modifications after wine consumption, comprising branched-chain amino acid (BCAA) metabolite (3-methyl-oxovalerate). Additionally, a possible interaction between alcohol and gut-related biomarkers has been identified. To our knowledge, this is the first time that this approach has been applied in a nutritional intervention with red wine. The results show the capacity of this approach to obtain a comprehensive metabolome picture including food metabolome and endogenous biomarkers of moderate wine intake

    Metabolomics reveals impaired maturation of HDL particles in adolescents with hyperinsulinaemic androgen excess.

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    Hyperinsulinaemic androgen excess (HIAE) in prepubertal and pubertal girls usually precedes a broader pathological phenotype in adulthood that is associated with anovulatory infertility, metabolic syndrome and type 2 diabetes. The metabolic derangements that determine these long-term health risks remain to be clarified. Here we use NMR and MS-based metabolomics to show that serum levels of methionine sulfoxide in HIAE girls are an indicator of the degree of oxidation of methionine-148 residue in apolipoprotein-A1. Oxidation of apo-A1 in methionine-148, in turn, leads to an impaired maturation of high-density lipoproteins (HDL) that is reflected in a decline of large HDL particles. Notably, such metabolic alterations occur in the absence of impaired glucose tolerance, hyperglycemia and hypertriglyceridemia, and were partially restored after 18 months of treatment with a low-dose combination of pioglitazone, metformin and flutamide

    Determinación de la longitud de la falla y velocidad de ruptura para terremotos de magnitud media

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    En el proceso de inversión de la función de directividad para la obtención de la longitud de falla y velocidad de mptura, en el caso de terremotos de magnitud media, es muy dificil obtener ambos parámetros independientemente y deben hacerse hipótesis sobre uno de los dos parámetros. Proponemos una solución a este problema mediante un proceso iterativo, que iniciamos con un valor hipotético de la velocidad de ruptura. El resultado final resulta ser independiente del valor de partida. Mediante este proceso hemos obtenido la longitud de falla y velocidad de ruptura de dos terremotos, el del 18-nov-1970, ocurrido en la Dorsal Oriental del Pacifico, y el del 4-jul-1966, ocurrido en la Dorsal Centro-Atlántica. Para el sismo del 18-nov-1970 los valores obtenidos en la b = (18 4- 1) km v = (2.3 f 0.1) kmls y para el sismo del 4-jul-1966 b = (19 f 2) km, v = (2.1 i 0.1) kmls. Las desviaciones tipicas de estos parámetros, del orden del 5-10 %, son inferiores a las obtenidas en trabajos previos

    Determinación de la longitud de la falla y velocidad de ruptura para terremotos de magnitud media

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    En el proceso de inversión de la función de directividad para la obtención de la longitud de falla y velocidad de mptura, en el caso de terremotos de magnitud media, es muy dificil obtener ambos parámetros independientemente y deben hacerse hipótesis sobre uno de los dos parámetros. Proponemos una solución a este problema mediante un proceso iterativo, que iniciamos con un valor hipotético de la velocidad de ruptura. El resultado final resulta ser independiente del valor de partida. Mediante este proceso hemos obtenido la longitud de falla y velocidad de ruptura de dos terremotos, el del 18-nov-1970, ocurrido en la Dorsal Oriental del Pacifico, y el del 4-jul-1966, ocurrido en la Dorsal Centro-Atlántica. Para el sismo del 18-nov-1970 los valores obtenidos en la b = (18 4- 1) km v = (2.3 f 0.1) kmls y para el sismo del 4-jul-1966 b = (19 f 2) km, v = (2.1 i 0.1) kmls. Las desviaciones tipicas de estos parámetros, del orden del 5-10 %, son inferiores a las obtenidas en trabajos previos

    La ciclicidad en los nuevos planes de estudio

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    Determinación de la longitud de la falla y velocidad de ruptura para terremotos de magnitud media

    No full text
    En el proceso de inversión de la función de directividad para la obtención de la longitud de falla y velocidad de mptura, en el caso de terremotos de magnitud media, es muy dificil obtener ambos parámetros independientemente y deben hacerse hipótesis sobre uno de los dos parámetros. Proponemos una solución a este problema mediante un proceso iterativo, que iniciamos con un valor hipotético de la velocidad de ruptura. El resultado final resulta ser independiente del valor de partida. Mediante este proceso hemos obtenido la longitud de falla y velocidad de ruptura de dos terremotos, el del 18-nov-1970, ocurrido en la Dorsal Oriental del Pacifico, y el del 4-jul-1966, ocurrido en la Dorsal Centro-Atlántica. Para el sismo del 18-nov-1970 los valores obtenidos en la b = (18 4- 1) km v = (2.3 f 0.1) kmls y para el sismo del 4-jul-1966 b = (19 f 2) km, v = (2.1 i 0.1) kmls. Las desviaciones tipicas de estos parámetros, del orden del 5-10 %, son inferiores a las obtenidas en trabajos previos
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