Sodium Thiosulfate For Recovery Of Bond Strength To Dentin Treated With Sodium Hypochlorite

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Introduction: The aim of this study was to evaluate the efficacy of sodium thiosulfate (Na2S2O3) for restoring adhesion to pulp chamber dentin treated with sodium hypochlorite (NaOCl) and EDTA. Methods: Sixty-three crowns of bovine incisors were cut to expose the dentin pulp chamber. The specimens were polished and randomly distributed into 9 groups (n = .7) according to the following protocols used: 0.9% sodium chloride for 30 minutes (negative control), 5.25% NaOCl for 30 minutes, 17% EDTA for 3 minutes, and 5.25% NaOCl for 1 minute (positive control). The other groups, after treatments with NaOCl and EDTA, were immersed in 0.5% or 5% Na2S2O3 for 1, 5, and 10 minutes or. just immersed in an inert solution for 10 minutes (0.9% sodium chloride). After drying the specimens, Scotchbond Multi-Purpose (3M ESPE, St Paul, MN) was applied to the pulp chamber dentin followed by Filtek Z250 composite (3M ESPE). Six rectangular slabs were obtained from each specimen, and the dentin/resin interface was tested by using a universal testing machine. The resulting data were submitted to 1-way analysis of variance and the Duncan test (P = .05). Results: There was a significant decrease in bond strength regarding NaOCl and EDTA (P < .05). When 5% Na2S2O3 was used for 10 minutes, the bond strength was found to be statistically equal to the negative control and higher than the positive control (P < .05). Conclusions: The use of Na2S2O3 can significantly increase the bond strength of composite resin to NaOCl/EDTA-treated dentin, allowing adhesive restorations to be immediately applied after endodontic treatment.42284288PAPESP [11/05302-0]CAPES PROEX [33003033008P8]Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES

P5-07-06: Effect of Angiotensin-(1-7) and Angiotensin II on T47D Breast Cancer Cells in the Proliferation and cAMP Production.

Abstract Angiotensin-(1-7) [Ang(1-7)] is a peptide hormone that produces opposite responses to those of the well-characterized peptide angiotensin II (Ang II). The main actions of Ang(1-7) and AII occur, respectively, via MAS and Angiotensin type 1 receptor (AT1R), respectively. Ang (1-7) has blood pressure and proliferative effects contrary to those of AngII, exhibits significant antiangiogenic activity and may be a novel therapeutic agent for lung cancer treatment targeting a specific AT(1-7) receptor (Soto-Pantoja et al., 2009). Here, our aim was to evaluate T47D breast cancer cell proliferation and cAMP production after treatment with Ang (1-7) and AngII.By means of the cyclic AMP (cAMP) competitive enzymeimmunoassay system we measured cAMP content of T47D cells. Cell proliferation was measured using incorporation of BrdU after Ang (1-7) and AII stimulation. Intracellular cAMP increasing drugs like Forskolin (FK) and isobutylmethylxanthine (IBMX) were also used to stimulate T47D cells.AII inhibited cAMP production (−39%) and A-(1-7) increased its formation (48%). Pattern of cAMP production was not altered when cells were previously incubated with FK. Results were inverted after IBMX incubation. In proliferation, opposite responses were observed for Ang II and Ang (1-7), that is, increasing (7.6 times) and decreasing (7.5 times) proliferation rates respectively. Also both peptides decreased proliferation when cells were stimulated by FK. Exactly the opposite happened after IBMX stimulation, both peptides increased proliferation. These results suggest that Ang (1-7) inhibits growth of T47D cells probably through inhibition of the via RAS/RAF/MAP quinase, what corroboraties previous findings. On the other hand, AngII can induce cell proliferation by an alternative via (CREB/growth factor). Therefore, Ang (1-7) seems to be not only a promising target to develop novel lung cancer therapeutic drugs, but it also might help to prevent the molecular changes that lead the normal breast epithelial cells to cancer initiation. Financial Support: FAPESP. Citation Information: Cancer Res 2011;71(24 Suppl):Abstract nr P5-07-06.</jats:p

Educação em saúde no serviço de radiologia: orientações para punção aspirativa de mama e tireóide

Trata-se de pesquisa convergente assistencial desenvolvida no Serviço de Radiologia de hospital-escola, cujo objetivoé conhecer as expectativas e dúvidas dos clientes submetidos à Punção Aspirativa por Agulha Fina de mama e tireoide. A coleta de dados ocorreu de setembro de 2010 a abril de 2011, por meio de 10 encontros educativos em sala de espera e entrevistas com 88 clientes. Os resultados apresentam: percepção dos clientes em face do exame,o câncer como estigma e a educação em saúde como estratégia de enfrentamento. Revelaram também presença de sentimento de medo do procedimento e do diagnóstico de câncer. A prática educativa em sala de espera é um espaçoque minimiza a ansiedade e permite a troca de experiências e conhecimentos entre profissional e cliente, bem como favorece uma rede de apoio entre os clientes. Caracteriza-se como espaço importante de atuação do enfermeiro no Serviço de Radiologia