Intermittent Hypoxia Is Associated With High Hypoxia Inducible Factor-1α but Not High Vascular Endothelial Growth Factor Cell Expression in Tumors of Cutaneous Melanoma Patients

Epidemiological associations linking between obstructive sleep apnea and poorer solid malignant tumor outcomes have recently emerged. Putative pathways proposed to explain that these associations have included enhanced hypoxia inducible factor (HIF)-1α and vascular endothelial growth factor (VEGF) cell expression in the tumor and altered immune functions via intermittent hypoxia (IH). Here, we examined relationships between HIF-1α and VEGF expression and nocturnal IH in cutaneous melanoma (CM) tumor samples. Prospectively recruited patients with CM tumor samples were included and underwent overnight polygraphy. General clinical features, apnea-hypopnea index (AHI), desaturation index (DI4%), and CM characteristics were recorded. Histochemical assessments of VEGF and HIF-1α were performed, and the percentage of positive cells (0, 75%) was blindly tabulated for VEGF expression, and as 0, 0-5.9, 6.0-10.0, >10.0% for HIF-1α expression, respectively. Cases with HIF-1α expression >6% (high expression) were compared with those 75% of cells was compared with those with <75%. 376 patients were included. High expression of VEGF and HIF-1α were seen in 88.8 and 4.2% of samples, respectively. High expression of VEGF was only associated with increasing age. However, high expression of HIF-1α was significantly associated with age, Breslow index, AHI, and DI4%. Logistic regression showed that DI4% [OR 1.03 (95% CI: 1.01-1.06)] and Breslow index [OR 1.28 (95% CI: 1.18-1.46)], but not AHI, remained independently associated with the presence of high HIF-1α expression. Thus, IH emerges as an independent risk factor for higher HIF-1α expression in CM tumors and is inferentially linked to worse clinical CM prognostic indicators.IA is supported by SEPAR (086/2014 and 595/2017). MM-G is supported by the Instituto de Salud Carlos III (PI16/01772) and cofinanced by the European Development Regional Find “A way to achieve Europe” (ERDF) and SEPAR (211/2012). ER-F is the recipient of a postdoctoral fellowship from “Fundación Científica de la Asociación Española Contra el Cáncer” and supported by FMM-2013/0075 of “Fundación Mutua Madrileña.” JR-P is supported by FIS 2014/1737 from the Spanish Ministry of Health. RF is supported in part by the Spanish Ministry of Economy and Competitiveness—Instituto de Salud Carlos III (FIS-PI14/00004); DG is supported by National Institutes of Health grant 1R01HL130984 and by the Herbert T. Abelson Chair in Pediatrics

Intermittent hypoxia is associated with high hypoxia inducible factor-1α but not high vascular endothelial growth factor cell expression in tumors of cutaneous melanoma patients

Epidemiological associations linking between obstructive sleep apnea and poorer solid malignant tumor outcomes have recently emerged. Putative pathways proposed to explain that these associations have included enhanced hypoxia inducible factor (HIF)-1α and vascular endothelial growth factor (VEGF) cell expression in the tumor and altered immune functions via intermittent hypoxia (IH). Here, we examined relationships between HIF-1α and VEGF expression and nocturnal IH in cutaneous melanoma (CM) tumor samples. Prospectively recruited patients with CM tumor samples were included and underwent overnight polygraphy. General clinical features, apnea-hypopnea index (AHI), desaturation index (DI4%), and CM characteristics were recorded. Histochemical assessments of VEGF and HIF-1α were performed, and the percentage of positive cells (0, 75%) was blindly tabulated for VEGF expression, and as 0, 0-5.9, 6.0-10.0, >10.0% for HIF-1α expression, respectively. Cases with HIF-1α expression >6% (high expression) were compared with those 75% of cells was compared with those with <75%. 376 patients were included. High expression of VEGF and HIF-1α were seen in 88.8 and 4.2% of samples, respectively. High expression of VEGF was only associated with increasing age. However, high expression of HIF-1α was significantly associated with age, Breslow index, AHI, and DI4%. Logistic regression showed that DI4% [OR 1.03 (95% CI: 1.01-1.06)] and Breslow index [OR 1.28 (95% CI: 1.18-1.46)], but not AHI, remained independently associated with the presence of high HIF-1α expression. Thus, IH emerges as an independent risk factor for higher HIF-1α expression in CM tumors and is inferentially linked to worse clinical CM prognostic indicators

The animal brain in 3D (first part)

El objetivo de este trabajo es hacer modelos del encéfalo en 3D en las especies de elección, perro y rata. Estos modelos se han llevado a cabo a partir de secciones encefálicas sin teñir, teñidas con técnicas histológicas y resonancias magnéticas. En estas secciones encefálicas se han identificado estructuras de interés. Esta información se ha utilizado para hacer un atlas 2D de cada una de las especies. Por último, del material tridimensional se han realizado impresiones 3D, fundamentalmente dirigidas al estudio de la neuroanatomía por estudiantes de los Grados en veterinaria y Ciencias Biológicas.The goal of this project is to obtain 3D models of the brain in the species of choice, dog and rat. These models proceed from unstained brain sections, stained sections with histological techniques and magnetic resonance imaging. Structures of interest have been identified in these brain sections. This information has been used to make a 2D atlas of each of the species. Finally, 3D impressions have been made of the three-dimensional material, fundamentally aimed at the study of neuroanatomy by students of the Degrees in Veterinary Medicine and Biological Sciences.Sección Deptal. de Anatomía y Embriología (Veterinaria)Fac. de VeterinariaFALSEsubmitte

The animals`brain in 3D (second part)

El trabajo resulta de interés para estudiantes y profesionales veterinarios y biólogos, así como para cualquierpersona interesada en el sistema nervioso de los animales.El concepto de “mascota” ha variado con el tiempo. Hace años, con este término, se identificaban perros, gatos, pequeños mamíferos o algunas aves domésticas. Hoy es relativamente normal encontrar, en domicilios particulares o en colecciones privadas, todo tipo de anfibios, reptiles, aves o mamíferos considerados como “exóticos”. Esta circunstancia ha generado la necesidad de un un conocimiento más amplio de ellos. Este mejor conocimiento resulta importante para los estudiantes del Grado en Veterinaria y en Ciencias Biológicas, los del Máster en Neurociencia, los del Diploma de Especialización en Animales Exóticos y Salvajes (ambos de la UCM) y, por supuesto, para profesionales veterinarios y biólogos. La neuroanatomía animal es una ciencia aún poco conocida. En nuestra propuesta, la elección de un reptil (la tortuga) y de un mamífero (el gato) no es casual. Es en los reptiles en los que aparece un primer esbozo de una corteza nueva que en los mamçiferos superiores constituirá lo que conocemos como hemisferios cerebrales. Esta corteza no existe en el grupo inmediatamente anterior, los anfibios. En cuanto al gat, es el animal doméstico por excelencia, junto con el perro, objeto de estudio en un proyecto anterior. Por las razones antes comentadas, el objetivo de la propuesta es poner a disposición de alumnos y profesionales un recurso educativo, en abierto, dinámico, y adaptado a la enseñanza virtual. Lo que plantea nuestro grupo es dar continuidad a un proyecto anterior (88/2021), con un mismo planteamiento, es decir, el de asociar resonancias magnéticas con imágenes de secciones cerebrales e impresiones 3D, para hacer una herramienta virtual, dinámica e interactiva. Dicha herramienta se puede alojar en el campus virtual de la UCM o en cualquier otro espacio virtual.Universidad Complutense de MadridDepto. de Anatomía y EmbriologíaDepto. de Biología CelularDepto. de Medicina y Cirugía AnimalFac. de VeterinariaFac. de Ciencias BiológicasFALSEsubmitte

Intermittent hypoxia is associated with high hypoxia inducible factor-1α but not high vascular endothelial growth factor cell expression in tumors of cutaneous melanoma patients

Epidemiological associations linking between obstructive sleep apnea and poorer solid malignant tumor outcomes have recently emerged. Putative pathways proposed to explain that these associations have included enhanced hypoxia inducible factor (HIF)-1α and vascular endothelial growth factor (VEGF) cell expression in the tumor and altered immune functions via intermittent hypoxia (IH). Here, we examined relationships between HIF-1α and VEGF expression and nocturnal IH in cutaneous melanoma (CM) tumor samples. Prospectively recruited patients with CM tumor samples were included and underwent overnight polygraphy. General clinical features, apnea-hypopnea index (AHI), desaturation index (DI4%), and CM characteristics were recorded. Histochemical assessments of VEGF and HIF-1α were performed, and the percentage of positive cells (0, 75%) was blindly tabulated for VEGF expression, and as 0, 0-5.9, 6.0-10.0, > 10.0% for HIF-1α expression, respectively. Cases with HIF-1α expression > 6% (high expression) were compared with those 75% of cells was compared with those with < 75%. 376 patients were included. High expression of VEGF and HIF-1α were seen in 88.8 and 4.2% of samples, respectively. High expression of VEGF was only associated with increasing age. However, high expression of HIF-1α was significantly associated with age, Breslow index, AHI, and DI4%. Logistic regression showed that DI4% [OR 1.03 (95% CI: 1.01-1.06)] and Breslow index [OR 1.28 (95% CI: 1.18-1.46)], but not AHI, remained independently associated with the presence of high HIF-1α expression. Thus, IH emerges as an independent risk factor for higher HIF-1α expression in CM tumors and is inferentially linked to worse clinical CM prognostic indicators

Intermittent hypoxia is associated with high hypoxia inducible factor-1α but not high vascular endothelial growth factor cell expression in tumors of cutaneous melanoma patients

Epidemiological associations linking between obstructive sleep apnea and poorer solid malignant tumor outcomes have recently emerged. Putative pathways proposed to explain that these associations have included enhanced hypoxia inducible factor (HIF)-1α and vascular endothelial growth factor (VEGF) cell expression in the tumor and altered immune functions via intermittent hypoxia (IH). Here, we examined relationships between HIF-1α and VEGF expression and nocturnal IH in cutaneous melanoma (CM) tumor samples. Prospectively recruited patients with CM tumor samples were included and underwent overnight polygraphy. General clinical features, apnea-hypopnea index (AHI), desaturation index (DI4%), and CM characteristics were recorded. Histochemical assessments of VEGF and HIF-1α were performed, and the percentage of positive cells (0, 75%) was blindly tabulated for VEGF expression, and as 0, 0-5.9, 6.0-10.0, >10.0% for HIF-1α expression, respectively. Cases with HIF-1α expression >6% (high expression) were compared with those 75% of cells was compared with those with <75%. 376 patients were included. High expression of VEGF and HIF-1α were seen in 88.8 and 4.2% of samples, respectively. High expression of VEGF was only associated with increasing age. However, high expression of HIF-1α was significantly associated with age, Breslow index, AHI, and DI4%. Logistic regression showed that DI4% [OR 1.03 (95% CI: 1.01-1.06)] and Breslow index [OR 1.28 (95% CI: 1.18-1.46)], but not AHI, remained independently associated with the presence of high HIF-1α expression. Thus, IH emerges as an independent risk factor for higher HIF-1α expression in CM tumors and is inferentially linked to worse clinical CM prognostic indicators

Si l@s estudiantes universitari@s no eligen ingenierías que las ingenierías vayan al colegio.

La pandemia provocada por el virus SARS-CoV-2 ha puesto de manifiesto la imperiosa necesidad de construir una sociedad basada en el conocimiento. Otros grandes retos sociales como son revertir el cambio climático y la conservación y/o recuperación del entorno natural hacen énfasis en la necesidad de un desarrollo sostenible e igualitario. En este sentido es necesario concienciar a las nuevas generaciones del papel primordial que deben jugar las ingenieras e ingenieros en el desarrollo futuro como motores de ese cambio necesario. Sin embargo, el número de estudiantes de ingenierías disminuye paulatinamente, siendo además profesiones copadas mayoritariamente por hombres. En el presente proyecto se plantea ir a colegios e institutos de la Comunidad de Madrid a realizar actividades que pongan de manifiesto lo que la ingeniería puede hacer por la sociedad, presentado por los y las estudiantes de ingeniería química de la UCM que servirán de referentes tanto femeninos como masculinos para las generaciones venideras.Oficina Universitaria ApS UCMDepto. de Ingeniería Química y de MaterialesFac. de Ciencias QuímicasTRUEsubmitte