Characterization of FMP35: A novel gene and its role in mitochondrial DNA stability

Mitochondria are essential organelles for all eukaryotic organisms with very few exceptions. The life-giving processes contributed by mitochondria are the end result of many proteins that are encoded within the mitochondria. Many nuclear encoded proteins give mitochondrial DNA (mtDNA) the high stability needed so that life can thrive. Saccharomyces cerevisiae (baker\u27s yeast) has historically been a model organism for mitochondrial function studies. These yeast are categorized as facultative anarobes; meaning that they are able to respire or ferment depending on media available. Functional mitochondria allow baker\u27s yeast to thrive on a 3-carbon medium (p+), while mitochondrial dysfunctions due to mtDNA defects do not allow growth on the same medium (p-). The ease of visualizing this phenotype and culturing these organisms has made S. cerevisiae an important tool for mitochondrial studies. Nuclear encoded proteins such as Abf2p and Ilv5p have been implicated in offering a degree of stability to mtDNA. Many nuclear proteins have been localized to mtDNA, creating an essential DNA-protein complex called a nucleoid. One protein that has been defined as a mitochondrial protein is Fmp35p. This is a novel protein that remains uncharacterized. A fmp35Δ::URA3 gene knockout yields a p- phenotype as illustrated by a respiration loss assay. Furthermore, a significant decrease in direct repeat recombination has been described by this study. A less significant increase in polymerase slippage within microsatellites has also been documented. It is the conclusion of this study that Fmp35p plays a role in a recombination pathway that gives rise to wild type yeast with a full complement of functional mtDNA. When this gene is defective and the protein is not produced yeast will not thrive

HYBRID MEMBRANE-PSA SYSTEM FOR SEPARATING OXYGEN FROMAR

A portable, non-cryogenic, oxygen generation system capable of delivering oxygen gas at purities greater than 98% and flow rates of 15 L/min or more is described. The system consists of two major components. The first component is a high efficiency membrane capable of separating argon and a portion of the nitrogen content from air, yielding an oxygen-enriched permeate flow. This is then fed to the second component, a pressure swing adsorption (PSA) unit utilizing a commercially available, but specifically formulated zeolite compound to remove the remainder of the nitrogen from the flow. The system is a unique gas separation system that can operate at ambient temperatures, for producing high purity oxygen for various applications (medical, refining, chemical production, enhanced combustion, fuel cells, etc...) and represents a significant advance compared to current technologies

Advanced proton-exchange materials for energy efficient fuel cells.

The ''Advanced Proton-Exchange Materials for Energy Efficient Fuel Cells'' Laboratory Directed Research and Development (LDRD) project began in October 2002 and ended in September 2005. This LDRD was funded by the Energy Efficiency and Renewable Energy strategic business unit. The purpose of this LDRD was to initiate the fundamental research necessary for the development of a novel proton-exchange membranes (PEM) to overcome the material and performance limitations of the ''state of the art'' Nafion that is used in both hydrogen and methanol fuel cells. An atomistic modeling effort was added to this LDRD in order to establish a frame work between predicted morphology and observed PEM morphology in order to relate it to fuel cell performance. Significant progress was made in the area of PEM material design, development, and demonstration during this LDRD. A fundamental understanding involving the role of the structure of the PEM material as a function of sulfonic acid content, polymer topology, chemical composition, molecular weight, and electrode electrolyte ink development was demonstrated during this LDRD. PEM materials based upon random and block polyimides, polybenzimidazoles, and polyphenylenes were created and evaluated for improvements in proton conductivity, reduced swelling, reduced O{sub 2} and H{sub 2} permeability, and increased thermal stability. Results from this work reveal that the family of polyphenylenes potentially solves several technical challenges associated with obtaining a high temperature PEM membrane. Fuel cell relevant properties such as high proton conductivity (>120 mS/cm), good thermal stability, and mechanical robustness were demonstrated during this LDRD. This report summarizes the technical accomplishments and results of this LDRD

SEMA4D compromises blood–brain barrier, activates microglia, and inhibits remyelination in neurodegenerative disease

AbstractMultiple sclerosis (MS) is a chronic neuroinflammatory disease characterized by immune cell infiltration of CNS, blood–brain barrier (BBB) breakdown, localized myelin destruction, and progressive neuronal degeneration. There exists a significant need to identify novel therapeutic targets and strategies that effectively and safely disrupt and even reverse disease pathophysiology. Signaling cascades initiated by semaphorin 4D (SEMA4D) induce glial activation, neuronal process collapse, inhibit migration and differentiation of oligodendrocyte precursor cells (OPCs), and disrupt endothelial tight junctions forming the BBB. To target SEMA4D, we generated a monoclonal antibody that recognizes mouse, rat, monkey and human SEMA4D with high affinity and blocks interaction between SEMA4D and its cognate receptors. In vitro, anti-SEMA4D reverses the inhibitory effects of recombinant SEMA4D on OPC survival and differentiation. In vivo, anti-SEMA4D significantly attenuates experimental autoimmune encephalomyelitis in multiple rodent models by preserving BBB integrity and axonal myelination and can be shown to promote migration of OPC to the site of lesions and improve myelin status following chemically-induced demyelination. Our study underscores SEMA4D as a key factor in CNS disease and supports the further development of antibody-based inhibition of SEMA4D as a novel therapeutic strategy for MS and other neurologic diseases with evidence of demyelination and/or compromise to the neurovascular unit

Summary report : universal fuel processor.

The United States produces only about 1/3 of the more than 20 million barrels of petroleum that it consumes daily. Oil imports into the country are roughly equivalent to the amount consumed in the transportation sector. Hence the nation in general, and the transportation sector in particular, is vulnerable to supply disruptions and price shocks. The situation is anticipated to worsen as the competition for limited global supplies increases and oil-rich nations become increasingly willing to manipulate the markets for this resource as a means to achieve political ends. The goal of this project was the development and improvement of technologies and the knowledge base necessary to produce and qualify a universal fuel from diverse feedstocks readily available in North America and elsewhere (e.g. petroleum, natural gas, coal, biomass) as a prudent and positive step towards mitigating this vulnerability. Three major focus areas, feedstock transformation, fuel formulation, and fuel characterization, were identified and each was addressed. The specific activities summarized herein were identified in consultation with industry to set the stage for collaboration. Two activities were undertaken in the area of feedstock transformation. The first activity focused on understanding the chemistry and operation of autothermal reforming, with an emphasis on understanding, and therefore preventing, soot formation. The second activity was focused on improving the economics of oxygen production, particularly for smaller operations, by integrating membrane separations with pressure swing adsorption. In the fuel formulation area, the chemistry of converting small molecules readily produced from syngas directly to fuels was examined. Consistent with the advice from industry, this activity avoided working on improving known approaches, giving it an exploratory flavor. Finally, the fuel characterization task focused on providing a direct and quantifiable comparison of diesel fuel and JP-8

Means to an End: An Assessment of the Status-blind Approach to Protecting Undocumented Worker Rights

This article applies the tenets of bureaucratic incorporation theory to an investigation of bureaucratic decision making in labor standards enforcement agencies (LSEAs), as they relate to undocumented workers. Drawing on 25 semistructured interviews with high-level officials in San Jose and Houston, I find that bureaucrats in both cities routinely evade the issue of immigration status during the claims-making process, and directly challenge employers’ attempts to use the undocumented status of their workers to deflect liability. Respondents offer three institutionalized narratives for this approach: (1) to deter employer demand for undocumented labor, (2) the conviction that the protection of undocumented workers is essential to the agency’s ability to regulate industry standards for all workers, and (3) to clearly demarcate the agency’s jurisdictional boundaries to preserve institutional autonomy and scarce resources. Within this context, enforcing the rights of undocumented workers becomes simply an institutional means to an end

HYBRID MEMBRANE-PSA SYSTEM FOR SEPARATING OXYGEN FROMAR

A portable, non-cryogenic, oxygen generation system capable of delivering oxygen gas at purities greater than 98% and flow rates of 15 L/min or more is described. The system consists of two major components. The first component is a high efficiency membrane capable of separating argon and a portion of the nitrogen content from air, yielding an oxygen-enriched permeate flow. This is then fed to the second component, a pressure swing adsorption (PSA) unit utilizing a commercially available, but specifically formulated zeolite compound to remove the remainder of the nitrogen from the flow. The system is a unique gas separation system that can operate at ambient temperatures, for producing high purity oxygen for various applications (medical, refining, chemical production, enhanced combustion, fuel cells, etc...) and represents a significant advance compared to current technologies

Why do situational interviews predict job performance? The role of interviewees’ ability to identify criteria

Purpose: This study aimed at shedding light on why situational interviews (SIs) predict job performance. We examined an explanation based upon the importance of interviewees’ Ability to Identify Criteria (ATIC, i.e., to read the targeted interview dimensions) for SI performance. Design/Methodology/Approach: Data were obtained from 97 interviewees who participated in a mock interview to train for future applications. This approach enabled us to conduct the SIs under standardized conditions, to assess interviewees’ ATIC, and at the same time, to collect job performance data from interviewee’s current supervisors. Findings: We found that interviewees’ ATIC scores were not only positively related to their interview performance, but also predicted job performance as rated by their supervisors. Furthermore, controlling for interviewees’ ATIC significantly lowered the relationship between performance in the SI and job performance. Implications: Better understanding of the mechanisms that underlie the criterion-related validity of SIs is crucial for theoretical progress and improving personnel selection procedures. This study highlights the relevance of interviewees’ ATIC for predicting job performance. It also underscores the importance of constructing interviews to enable candidates to show their criterion-relevant abilities. Originality/Value: This study shows that interviewees’ ATIC contributes to a better understanding of why the SI predicts job performance

Why do Situational Interviews Predict Job Performance? The Role of Interviewees' Ability to Identify Criteria

Purpose: This study aimed at shedding light on why situational interviews (SIs) predict job performance. We examined an explanation based upon the importance of interviewees' Ability to Identify Criteria (ATIC, i.e., to read the targeted interview dimensions) for SI performance. Design/Methodology/Approach: Data were obtained from 97 interviewees who participated in a mock interview to train for future applications. This approach enabled us to conduct the SIs under standardized conditions, to assess interviewees' ATIC, and at the same time, to collect job performance data from interviewee's current supervisors. Findings: We found that interviewees' ATIC scores were not only positively related to their interview performance, but also predicted job performance as rated by their supervisors. Furthermore, controlling for interviewees' ATIC significantly lowered the relationship between performance in the SI and job performance. Implications: Better understanding of the mechanisms that underlie the criterion-related validity of SIs is crucial for theoretical progress and improving personnel selection procedures. This study highlights the relevance of interviewees' ATIC for predicting job performance. It also underscores the importance of constructing interviews to enable candidates to show their criterion-relevant abilities. Originality/Value: This study shows that interviewees' ATIC contributes to a better understanding of why the SI predicts job performance

The emerging solid state transformer and its impact on the electric power grid

[Extract] The most important impact of power electronics on our society in the last 50 years has been the elimination of the 60 Hz AC power delivery system for consumer electronics products. Central to this achievement is the use of silicon power devices and pulse width modulation (PWM) techniques in delivering regulated AC and DC power to low voltage loads in devices such as LEDs and computers. These solid state power electronics have provided our society numerous benefits, including high quality power and substantial energy savings, and also represent the indispensible core technology for integrating renewable energies such as wind and solar [4] into our power grid