A phosphorus NMR study of the reaction products of cis-diamminedichloroplatinum (II) with a double-helical oligonucleotide and with DNA

AbstractThe structural distortion of oligonucleotides upon cis-PtCl2(NH3)2{d(T-C-T-C-G-G-T-C-T'-C-N7(5) N7(6)} reveals shifting of 4 phosphorus resonances due to platination. 3 Resonances could be assigned by selective 31P-irradiation, showing P(6) (P between the two Gs) to be shifted 1.5 ppm to low field. In the concomitant double strands P(6) is shifted 0.9 ppm to lower field. A similar peak has been observed in platinated salmon sperm DNA (37°C), indicating that Pt-binding to GpG-fragments in DNA is similar to that found for the decanucleotide, so the distortion of DNA might be comparable

A C3′ modified nucleotide. The difluorophosphonate function, a phosphate mimic, governs the conformational behaviour of the ribofuranose

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Adenylyl‐uridine. Assignment and analysis of the 270 Mhz fast Fourier transform proton magnetic resonance spectrum

The fast Fourier‐transform proton magnetic spectrum of Adenylyl‐uridine (ApU) was taken at 270 MHz. The complex group of signals due to the ribose protons could be analyzed to yield chemical shifts. 1H1H and 1H31P coupling constants. The assignment of the various peaks was based mainly on the known assignment of the N6‐monomethylated analogue m6ApU, see C. Altona et al. Nature 247, 558 (1974). Best values of the chemical shifts and coupling constants of ApU were obtained by carrying out a series of computer simulations of line shapes and intensities. Copyright © 1974 WILEY‐VCH Verlag GmbH & Co. KGaA, WeinheimSCOPUS: ar.jinfo:eu-repo/semantics/publishe