effects of temperature, pH and sugar binding on the structures of lectins ebulin f and selfd

Producción CientíficaEbulin f and SELfd are two lectins of Sambucus ebulus L. that show different stability and digestibility properties in gastric fluid due to their structural differences which may explain their different toxicological profiles. The main aim was to determine the effects of pH, temperature and sugar binding on the intrinsic structures of both proteins by fluorescence analyses. Quenching experiments were conducted, under different pH and temperature conditions, with acrylamide (uncharged) and iodide (charged), to study the possible changes of their intrinsic fluorescence. Results revealed that the native structure of SELfd is more folded than that of ebulin f. At pH 2.0, ebulin f displayed a more open structure than at neutral pH. It can be concluded that this is the main reason why ebulin f is accessible to pepsin action and more sensitive to degradation, in contrast to SELfd as we reported previously.Grupo de Excelencia GR106UVa-GIR Inmunotoxinas AntitumoralesConvenio Consejería de Sanidad JCy

Data-Driven Aggregation Control for Thermoelectric Loads in Demand Response

Within the concept of a smart grid, aggregators have the task of coordinating the behavior of large sets of Distributed Energy Resources, each of them offering small power/energy capacities, which help to balance the power grid and can serve as providers of services. Adequate coordination strategies are required to optimally exploit these resources in the ancillary services market. However, deriving model-based control policies for them is complex due to the heterogeneity and uncertainty related to the large set of associated agents. Then, a data-driven model is an adequate solution for this sort of situation. This paper presents the application of the Youla-Kucera Data-Driven Control strategy for the development of an aggregator to regulate the power consumption of a set of thermoelectric refrigerators, avoiding the modeling process and directly designing a controller from data. A detailed simulation framework was executed to verify the validity of the proposed methodology. It is shown that the derived aggregator is able to offer frequency containment reserves service, achieving the required settling time of 30 seconds and with a tracking error below 4.7%. Copyright (c) 2022 The Authors. This is an open access article under the CC BY-NC-ND license (https://creativecommons.org/licenses/by-nc-nd/4.0/

Estudios en torno a la producción de sentido a partir de la memoria colectiva en comunidades negras del pacífico nariñense

El objetivo de este trabajo es analizar los trabajos académicos producidos en torno a la memoria colectiva y el conflicto armado de las comunidades de la costa pacífica nariñense. Usando una metodología de enfoque hermenéutico mediante la técnica de investigación de archivos, y con la construcción de fichas tipo RAE como instrumento de registro para la recolección de información. Como conclusión podemos decir que la construcción de la memoria en esta zona del país, ha tenido entre sus retos articular factores como la migración y los medios de expresión, desplazamientos culturales de la tradición oral narrativa a los medios escritos, a la música y al teatro, otros trabajos centran sus análisis en la educación y alfabetización como estrategias para deshegemonizar la tradición oral como medio de construcción de la memoria por antonomasia y que ha permitido que no haya una memoria colectiva sólida.Abstract Using a hermeneutic approach methodology through a file research technique and building a Specialized Analytic Abstract (RAE by its initials in Spanish) chart, the objective of the monograph is quite clear: To understand the academic production that surrounds the Collective Memory in Tumaco town - Nariño. The construction of the Collective Memory in Tumaco has had several challenges such as migration of expression forms and media such as migration from an oral tradition to written media, music or theater which, along with education literacy, have been the most appropriate strategies for getting Oral Tradition dehegemonized, thus because it has been the main memory building medium and has allowed a disperse collective memory

LAS 2000: Introduction to Latin American Studies

Q2S Enhancing Pedagogy stipends were used to support the formation of a Faculty Learning Community to develop a new course, LAS 2000. Five faculty members from the College of Arts and Letters and the College of Social and Behavioral Sciences collectively designed and produced an fully online, team taught semester course. LAS 2000 is an introductory course to Latin American Studies, a requirement in the LAS Minor Program. The course introduces students to the study of Latin America from various perspectives: Anthropology, Sociology, Geography, Literature, and Theater/Film, with the goal of fostering greater cross-cultural understanding of the region and enhancing diverse, global perspectives. The course is open to students of any major and has no prerequisites

Unexpected Toxicity of Green Tea Polyphenols in Combination with the Sambucus RIL Ebulin

Abstract: The safety of concentrated food complements intake is a major health concern. It has been well established that green tea polyphenols (GTPs) consumption promotes healthy effects. However, the ingestion of large amounts of GTPs is a matter of controversy due to reported adverse effects. We underwent a preliminary exploration of the effects of the oral administration of a standardized concentrated GTPs preparation on mice which suffered from reversible intestinal derangement promoted by sublethal amounts of the antiribosomal lectin ebulin f from dwarf elder (Sambucus ebulus L.). Neither independent oral administration of 30 mg/kg body weight Polyphenon 60 nor intraperitoneal administration of 2.5 mg/kg body weight ebulin f triggered lethal toxicity. In contrast, the simultaneous administration of these same doses of both Polyphenon 60 and ebulin f triggered an important and unexpected synergistic toxic action featured by the biphasic reduction of weight, which continued after eight days, reaching a reduction of 40%. Lethality appeared 2 days after the onset of the combined treatment and reached more than 50% after 10 days.Junta de Castilla y León (GR106 and Consejería de Sanidad) and UVa-GIR.Depto. de Farmacia Galénica y Tecnología AlimentariaFac. de FarmaciaTRUEpu

Improving vocabulary acquisition by means of listening activities

This thesis deals with the problem of vocabulary by means of listening. In particular, the question we are trying to answer is this: How can current teaching strategies can be enhanced to include listening skills thereby improving the vocabulary level in USB modern language students? The literature research process allowed us to explore a number of key terms and second language acquisition theories among which Stephen Krashen’s second language acquisition theory (SLA), and its five hypothesis, prove to be coherent to the theoretical framework the work of Paul Nation and Jonathan Newton, who follow Krashen’s ideas serve as a guide and from it we adapted a number of activities from Nation and Newton to improve listening and increase students’ vocabulary levels. These activities have been adapted to the Colombian context and, specifically the Modern Language Program at San Buenaventura University

Retinol and alpha-Tocopherol Contents, Fat Color, and Lipid Oxidation as Traceability Tools of the Feeding System in Suckling Payoya Kids

Simple Summary In Spain, goat farms are mainly oriented to milk production, although kid meat contributes to their sustainability, particularly in autochthonous breeds such as Payoya. Usually, kids are fed artificial milk until slaughter, allowing the use of goat milk for the commercialization of cheese, but several studies indicate that feeding kids natural milk improve the quality of their meat. The aim of the present study was to find traceability markers to discriminate between kids that are fed natural milk (with different goat management systems) and those fed a milk replacer. For this purpose, we proposed the quantification of retinol and alpha-tocopherol contents in plasma and fat, the amount of kidney fat, lipid oxidation, and some fat color parameters as potential markers. The results showed that plasma retinol concentrations were higher in kids fed feeding systems with synthetic vitamins. The plasma alpha-tocopherol concentrations were higher in kids fed grass-based feeding systems (which contain the natural forms of these vitamins). A dilution effect was shown for the retinol concentration in fat. Collectively, the analyzed variables allowed a discriminant analysis to correctly classify kids according to their feeding system and could ensure traceability to consumers. The effects of Payoya kid feeding systems on the fat-soluble vitamin (retinol/alpha-tocopherol) contents, fat content, fat color, and the oxidation index were evaluated to determine their potential for use as feeding system traceability tools. Four groups of Payoya kids (55 animals in total) fed milk exclusively were studied: a group fed a milk replacer (MR) and three groups fed natural milk from dams reared with different management systems (mountain grazing (MG), cultivated meadow (CM) and total mixed ration (TMR)). Kids were slaughtered around one month of age and 8 kg of live weight. Kids from the MG and CM groups presented lower retinol (5.56 and 3.72 mu g/mL) and higher alpha-tocopherol plasma (11.43 and 8.85 mu g/mL) concentrations than those from the TMR and MR groups (14.98 and 22.47 mu g/mL of retinol; 2.49 and 0.52 mu g/mL of alpha-tocopherol, respectively) (p < 0.001). With respect to fat, kids with a higher intramuscular fat percentage (CM and TMR groups) had lower retinol contents (16.52 and 15.99 mu g/mL, respectively) than kids from the MG and MR groups (26.81 and 22.63 mu g/mL, respectively) (p < 0.001). A dilution effect of vitamins on fat was shown: the higher the amount of fat, the lower the vitamin concentrations, the higher the lipid oxidation index (MDA), and the lower the SUM (absolute value of the integral of the translated spectra between 450 and 510 nm). A discriminant analysis that included all studied variables showed that 94.4% of the kids were classified correctly according to their feeding system and could allow traceability to the consumer

On-line Parameter Estimation of the Polarization Curve of a Fuel Cell with Guaranteed Convergence Properties: Theoretical and Experimental Results

In this paper, we address the problem of online parameter estimation of a Proton Exchange Membrane Fuel Cell (PEMFC) polarization curve, that is the static relation between the voltage and the current of the PEMFC. The task of designing this estimator -- even off-line -- is complicated by the fact that the uncertain parameters enter the curve in a highly nonlinear fashion, namely in the form of nonseparable nonlinearities. We consider several scenarios for the model of the polarization curve, starting from the standard full model and including several popular simplifications to this complicated mathematical function. In all cases, we derive separable regression equations -- either linearly or nonlinearly parameterized -- which are instrumental for the implementation of the parameter estimators. We concentrate our attention on on-line estimation schemes for which, under suitable excitation conditions, global parameter convergence is ensured. Due to these global convergence properties, the estimators are robust to unavoidable additive noise and structural uncertainty. Moreover, their on-line nature endows the schemes with the ability to track (slow) parameter variations, that occur during the operation of the PEMFC. These two features -- unavailable in time-consuming off-line data-fitting procedures -- make the proposed estimators helpful for on-line time-saving characterization of a given PEMFC, and the implementation of fault-detection procedures and model-based adaptive control strategies. Simulation and experimental results that validate the theoretical claims are presented.Comment: 16 pages, 18 figures, requires IEEEtran.cls 2015/08/26 version V1.8

EVALUATION OF THE EFFICACY OF A HYDROGEN PEROXIDE DISINFECTANT

Objective: To evaluate the efficacy of a disinfectant based on hydrogen peroxide.Methods: The method used to assess the efficacy of the disinfectant was the agar plate technique. With this procedure, it was possible to determine the percentage of inhibition of the high-level disinfectant of STERIS against four microorganisms, i.e., Pseudomonas aeruginosa ATCC 9027, Staphylococcus aureus (Beta-Hemolytic 227), Salmonella choleraesuis (Kuznedorf CMDM 074), and Bacillus subtilis (ATCC 6633). The effectiveness of five disinfectant concentrations (0.02%, 0.04%, 0.08%, 1%, and 2%) was determined and evaluated in three different times 5, 10, and 15 min, for vegetative strains and 3, 6, and 9 h for the sporulated strain.Results: According to the experimental test, the reduction of the microbial population was, on average, 100% for the disinfectant concentrations of 0.08%, 1%, and 2%.Conclusion: The results obtained demonstrated that the high-level disinfectant of STERIS based on hydrogen peroxide is 100% effective when the concentration recommended by the commercial house (2%) is used in the shortest time exposure to disinfectant. The minimum level of effectiveness was 0.08%; however, if lower concentrations are used, destruction of the microorganisms is not guaranteed

Virus entericos segmentados en materia fecal de diferentes especies convivientes en la provincia de La Rioja.

Fil: Cuffia, V.I. Instituto Universitario de Ciencias de la Salud. Fundación Barceló; Argentina.Fil: Silvera, C.M.A. Instituto Universitario de Ciencias de la Salud. Fundación Barceló; Argentina.Fil: Diaz Ariza, M. Instituto Universitario de Ciencias de la Salud. Fundación Barceló; Argentina.Fil: Cordoba, Patricia A. Instituto Universitario de Ciencias de la Salud. Fundación Barceló; Argentina.Virus entericos segmentados en materia fecal de diferentes especies conviviente